Detection of Brucella melitensis in semen using the polymerase chain reaction assay

Amin, Adel S.; Hamdy, Mahmoud; Ibrahim, Asmaa

doi:10.1016/s0378-1135(01)00401-1

Cited by 67 publications

(52 citation statements)

References 9 publications

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“…Analytical sensitivity observed in the other organs, semen and urine samples were equal to 3x10 2 CFU/mL, similar to the results by Amim et al (2001). Sensitivity of 1.0x10 0 CFU/mL was observed in pools of semen samples obtained from dogs naturally infected by B. canis (Keid, 2007).…”

supporting

confidence: 85%

“…The low percentage of isolation was justified by the low sensitivity of modified Thayer-Martin agar for semen culture (Manterola et al, 2003). The results of microbiological culture of infected animals in the present study were lower compared with those of a published report (Biberstein et al, 1963). This difference may be explained by the culture medium used in the trial.…”

contrasting

confidence: 79%

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Adaptation and evaluation of polymerase chain reaction for Brucella ovis detection in semen, urine and organs of rams experimentally infected

Nozaki

Salgado

Lira

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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supporting

confidence: 85%

contrasting

confidence: 79%

Adaptation and evaluation of polymerase chain reaction for Brucella ovis detection in semen, urine and organs of rams experimentally infected

Nozaki

Salgado

Lira

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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“…The non-uniform N. caninum distribution and the limited amount of parasite DNA present in fluids and tissues have been reported in naturally or experimentally infected adult cattle [11,12]. Moreover, infected seropositive cattle testing negative for specific DNA in the semen, as assessed by PCR, have been reported for other infectious agents such as Brucella melitensis [13] or bovine herpesvirus type 1 [14].…”

Section: Discussionmentioning

confidence: 99%

Experimental neosporosis in bulls: Parasite detection in semen and blood and specific antibody and interferon-gamma responses

et al. 2007

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Aim: To investigate the presence of Neospora caninum in semen and blood, and the development of specific antibody and interferon-gamma (IFN-g) responses in experimentally infected bulls. Methods: Eight bulls were intravenously infected with 10 8 live N. caninum tachyzoites of NC-1 isolate. The presence of N. caninum in semen and blood was assessed using a nested-PCR procedure. PCR-positive semen samples were bioassayed using a BALB/c nu/nu mouse model. Specific anti-N. caninum antibody and IFN-g responses were also examined. In parallel, eight seronegative bulls were studied as non-infected controls. All bulls were monitored for 26 weeks. Results: All eight experimentally infected bulls showed N. caninum DNA in their semen and/or blood samples at some time during the course of the study. Parasite load in semen ranged from 0.1 to 14.5 parasites/ml (mean 6.0). N. caninum could not be detected in BALB/c nu/nu mice inoculated with PCR-positive semen samples. A significant increase in mean serum specific IgM antibody response to N. caninum was detected between 10 and 28 days post-infection (p.i.). Serum specific IgG, IgG1, and IgG2 antibody levels in experimentally infected bulls were significantly different after 21, 10, and 14 days p.i. as compared to controls, respectively. Specific anti-N. caninum IgG were detected in seminal plasma from infected bulls and values obtained were different from controls after 25 days p.i. Mean specific IFN-g responses in experimentally infected bulls were significantly higher than controls 3 days p.i. Conclusions: This is the first study to report the presence of N. caninum DNA in the semen and blood of experimentally infected bulls. Our observations indicate an intermittent presence of N. caninum in low numbers in semen and associated with chronic stage of the infection. This study is also the first to report the detection of anti-N. caninum IgG in seminal plasma of experimentally infected bulls. #

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“…However, there is an appreciable risk in bull semen that is utilized in artificial insemination due to the intrauterine placement of the semen compared to vaginal deposition during natural servicing [72–74] . Semen from sero-positive bulls has been found to contain B. melitensis [75]. In an experimental study of mature bulls, inoculation with B. abortus vaccine strain 19 led to the persistent shedding of the this bacteria in semen [76].…”

Section: Pathogenesis and Clinical Presentation In Animalsmentioning

confidence: 99%

Comparisons of brucellosis between human and veterinary medicine

Hull

Schumaker

2018

Infection Ecology & Epidemiology

151

129

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Brucellosis is the world’s most widespread zoonosis, but also ranks as one of the seven most neglected diseases, according to the World Health Organization. Additionally, it is recognized as the world’s most common laboratory-acquired infection. There are a reported 500,000 incident cases of human brucellosis per year. However, true incidence is estimated to be 5,000,000 to 12,500,000 cases annually. Once diagnosed, focus is directed at treating individual patients with antibiotic regimes, yet overall neglecting the animal reservoir of disease. Countries with the highest incidence of human brucellosis are Syria (1,603.4 cases per 1,000,000 individuals), Mongolia (391.0), and Tajikistan (211.9). Surveillance on animal populations is lacking in many developed and developing countries. According to the World Animal Health Information Database, Mexico had the largest number of reported outbreaks, 5,514 in 2014. Mexico is followed by China (2,138), Greece (1,268), and Brazil (1,142). The majority of these outbreaks is Brucella abortus, the etiologic agent of bovine brucellosis. Brucellosis is an ancient disease that still plagues the world. There are still knowledge gaps and a need for better diagnostics and vaccines to make inroads towards control and eradication.

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Detection of Brucella melitensis in semen using the polymerase chain reaction assay

Cited by 67 publications

References 9 publications

Adaptation and evaluation of polymerase chain reaction for Brucella ovis detection in semen, urine and organs of rams experimentally infected

Adaptation and evaluation of polymerase chain reaction for Brucella ovis detection in semen, urine and organs of rams experimentally infected

Experimental neosporosis in bulls: Parasite detection in semen and blood and specific antibody and interferon-gamma responses

Comparisons of brucellosis between human and veterinary medicine

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