Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction

Khamesipour, Faham; Doosti, Abbas; Emadi, Mohsen Fard; Awosile, Babafela

doi:10.2478/bvip-2014-0081

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…Of these, 57.1% (8/14) did not identify any significant factor associated with leptospirosis. Among the studies that observed variables significantly associated with canine leptospirosis, the main risk factors observed were age: older than 4 years (Chou et al ., 2014) and younger than 1 year (Zaidi et al ., 2018), seasonality (Chou et al ., 2014), stray as opposed to a pet dog living in a household (Roach et al ., 2010; Khamesipour et al ., 2014; Paz et al ., 2015), and dogs that lived in urban areas, sharing a common area with human beings and exhibiting a history of contact with rats (Goh et al ., 2019). Detailed information on the risk factor analysis performed in these studies is shown in Table 5.…”

Section: Resultsmentioning

confidence: 99%

Canine leptospirosis in stray and sheltered dogs: a systematic review

Costa

Colocho

Pereira

et al. 2022

Anim. Health. Res. Rev.

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The aim of this systematic review was to investigate the prevalence of leptospirosis among stray and sheltered dogs worldwide. Six databases were searched, which resulted in the retrieval of 476 articles. Sixty articles were selected for analysis according to 10 quality criteria. Among the selected papers, 26 papers [43.4% (26/60)] met five of the 10 quality criteria established, 10 papers [16.7% (10/60)] met three criteria, nine papers [15.0% (9/60)] met four criteria, six papers [10.0% (6/60)] met six criteria, four papers [6.7% (4/60)] met eight criteria, and three papers [5.0% (3/60)] met nine of the 10 criteria, whereas two papers [1.7% (1/60)] met two and seven [1.7% (1/60)] criteria. Publications originated mainly from the Americas [45.0% (27/60)] and in the last 16 years (2003–2019) [81.7% (49/60)], and most of the sampled dogs were stray dogs [65.0% (39/60)]. The most commonly used diagnostic test for leptospirosis was the microscopic agglutination test [78.4% (47/60)] followed by polymerase chain reaction [21.7% (13/60)], and the most common serovars were Canicola [71.4% (35/49)], Icterohaemorrhagiae [65.3% (32/49)], Grippotyphosa [40.8% (20/49)], and Pomona [40.8% (20/49)]. In conclusion, our results showed that Leptospira spp. are present in unowned dogs worldwide; however, the low-methodological quality of the recovered cross-sectional studies precluded a meta-analysis.

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Section: Resultsmentioning

confidence: 99%

Canine leptospirosis in stray and sheltered dogs: a systematic review

Costa

Colocho

Pereira

et al. 2022

Anim. Health. Res. Rev.

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“…PCR assay is a specific and sensitive choice for the detection of different bacterial agents [2,22,23]. In this study, 9.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of <i>Brucella melitensis</i> in sheep and goat milk in Iran

Shakerian¹,

Deo²,

Rahimi³

et al. 2016

Trop. J. Pharm Res

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“…In the only PCR-based study in Iran, 14 out of 94 (14.9%) tested blood samples from companion dogs of Isfahan and Shahrekord cities were reported to be positive by conventional-PCR (32). As the PCR products in the latter study were not confirmed by nucleotide sequencing and the dogs did not show any sign of brucellosis, these results should be taken with caution.…”

Section: Discussionmentioning

confidence: 87%

Detection ofBrucellaAntibodies in Dogs From Rural Regions of Hamedan, Iran

Gharekhani

Sazmand²

2019

Avicenna J Clin Microbiol Infect

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BackgroundDogs play an important role in the maintenance and transmission of several zoonotic pathogens. In the case of Brucella spp., the close contact of dogs with humans and livestock might cause zoonotic diseases and economic losses due to abortions and stillbirths in animals (1, 2). Brucellosis is prevalent in some regions of Iran including Hamedan where recently the first human case of infection with Brucella canis in the country was reported (3,4).Canine brucellosis caused by B. canis, a Gram-negative facultative intracellular bacterium, is a neglected zoonosis. B. canis in dogs was firstly reported in the United States in 1966, and since then the bacterium has been detected globally, presenting itself in various forms (1,5). The predominant signs of disease in dogs, the major hosts, are abortion, infertility, stillbirth, lymphadenitis, epididymitis, orchitis, and prostatitis (2). Transmission of infection occurs via ingestion of contaminated materials or venereal routes. Diagnosis is usually based on the isolation of causative agent and/or serology techniques (6). B. canis has been reported in humans and wild canids, as well (7). Although B. canis is the important cause of brucellosis in dogs, infection with B. abortus, B. melitensis, and B. suis has also been reported (8). Considering that infected dogs can shed organisms into the environment via urine and vaginal discharges and secretions, aborted materials or feces, they play a significant role in the maintenance of Brucella spp. and its possible transmission to other dogs, cattle, and humans (8,9).In an earlier research from Hamedan, the rate of brucellosis was detected 3% and 4.6% in sheep and goats, respectively (10). Moreover, seroprevalence rates of 8.1% in veterinarians, 15% in slaughterhouse workers, and 17% in butchers have been reported (11). The incidence rate of human brucellosis in Hamedan province is 31-41 per 100 000 population, which is classified as "very high" in Iran (12). Recurrence rate of human brucellosis in this region is calculated as 6.45% (13) and direct contact of human with infected animals is the main risk factor for the disease (4,11,12,14).In Iran, there is scanty knowledge about canine brucellosis with no information from Hamedan. Therefore, the aim of the current cross-sectional study was to determine the rate of Brucella sp. infection in dogs from Hamedan, West

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Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction

Cited by 6 publications

References 14 publications

Canine leptospirosis in stray and sheltered dogs: a systematic review

Canine leptospirosis in stray and sheltered dogs: a systematic review

Molecular detection of <i>Brucella melitensis</i> in sheep and goat milk in Iran

Detection ofBrucellaAntibodies in Dogs From Rural Regions of Hamedan, Iran

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