Detection of C-reactive protein based on magnetic nanoparticles and capillary zone electrophoresis with laser-induced fluorescence detection

Lin, Yi Jyun; Yang, Jian; Shu, Ting Yu; Lin, Ting; Chen, Yen Yi; Su, Mei Yu; Li, Wen Jie; Liu, Mine-Yine

doi:10.1016/j.chroma.2013.09.042

Cited by 16 publications

(7 citation statements)

References 20 publications

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“…An immunoassay based on the use of magnetic nanobeads was used for efficient sample pretreatment to determine haptoglobin, a biomarker for Alzheimer's disease [200], and c‐reactive protein (CRP), a biomarker for acute and chronic inflammation [201] in human plasma by CZE with laser‐induced fluorescence detection. Haptoglobin and CRP were immobilized on the magnetic nanobeads and reacted with the purified anti‐haptoglobin/CRP antibody, and the labeled secondary antibodies reacted with the complex.…”

Section: Immunoaffinity Methodsmentioning

confidence: 99%

Sample preparation and fractionation techniques for intact proteins for mass spectrometric analysis

Thomas

Thacker

Schug

et al. 2020

J of Separation Science

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The analysis of proteins in biological samples is highly desirable, given their connection to myriad biological functions and disease states, as well as the growing interest in the development of protein‐based pharmaceuticals. The introduction and maturation of “soft” ionization methods, such as electrospray ionization and matrix‐assisted laser desorption/ionization, have made mass spectrometry an indispensable tool for the analysis of proteins. Despite the availability of powerful instrumentation, sample preparation and fractionation remain among the most challenging aspects of protein analysis. This review summarizes these challenges and provides an overview of the state‐of‐the‐art in sample preparation and fractionation of proteins for mass spectrometric analysis, with an emphasis on those used for top‐down proteomic approaches. Biological fluids, particularly important for clinical and pharmaceutical applications and their characteristics are also discussed. While immunoaffinity‐based methods are addressed, more attention is given to non‐immunoaffinity‐based methods, such as precipitation, coacervation, size exclusion, dialysis, solid‐phase extraction, and electrophoresis. These techniques are presented in the context of a significant number of studies where they have been developed and utilized.

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Section: Immunoaffinity Methodsmentioning

confidence: 99%

Sample preparation and fractionation techniques for intact proteins for mass spectrometric analysis

Thomas

Thacker

Schug

et al. 2020

J of Separation Science

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“…However, this treatment usually infers further analytical use of the conjugate. For magnetic nanoparticles magnetic separation is the possible method of choice . The type of interaction (electrostatic, covalent coating, etc.)…”

Section: Ce Methodology In Nano–bio Researchmentioning

confidence: 99%

“…Depending on their fluorescent properties, they can be detected either directly (e.g. down to 2 × 10 −10 M for γ‐Fe 2 O 3 ‐silica core‐shell nanoparticles functionalized with PEG ) or after surface modification by the attachment of a fluorescence probe . Additionally, fluorescence measurements based on use of inverted fluorescence microscope have been applied to monitor the self‐assembling of QDs with various biomolecules inside the capillary and also with QDs added to BGE (as a probe), to determine various biomolecules [; see also a commentary on this topic by Vaculovicova et al.…”

Section: Ce Methodology In Nano–bio Researchmentioning

confidence: 99%

Characterization of interactions of metal‐containing nanoparticles with biomolecules by CE: An update (2012–2016)

2017

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The methodological developments and applications of CE related to studying biotransformations of metal-based nanoscale particles of impending medicinal use are overviewed. This is an update to a previous review article (Aleksenko, S. S., Shmykov, A. Y., Oszwałdowski, S., Timerbaev, A. R., Metallomics 2012, 4, 1141-1148) and it covers the research papers published within the last five years. As was anticipated in that review, CE can now be seen as a customary technique in the analysis of biomolecular interactions that exert an impact on the mechanism of action of nanoparticles, comprising metabolism, delivery, cell processing, and targeting. Different ways by which the CE method is applied for such monitoring, including conjugation mode, sample preparation, separation, and detection, are critically assessed. Special emphasis is put on examinations using inductively coupled plasma MS detection recent advent of which to the area made CE a versatile speciation tool for biomedical studies of nanomaterials containing metals.

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“…Lin et al used protein G modified MNP in CE with LIF detection to analyze C-reactive protein (CRP), which is a wellknown biomarker of acute and chronic inflammatory. The protein G modified MNP that was formed covalently binds to anti-CRP antibody 1, CRP, and FITC labeled anti-CRP antibody 2, and CRP was directly analyzed by CE with LIF detection, without elution of CRP from the MNP surface [66], as shown in Fig. 4.…”

Section: Application Of Nanoparticles In Cementioning

confidence: 99%

Analysis and applications of nanoparticles in capillary electrophoresis

Ban

Yoo

Song

2015

Talanta

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Detection of C-reactive protein based on magnetic nanoparticles and capillary zone electrophoresis with laser-induced fluorescence detection

Cited by 16 publications

References 20 publications

Sample preparation and fractionation techniques for intact proteins for mass spectrometric analysis

Sample preparation and fractionation techniques for intact proteins for mass spectrometric analysis

Characterization of interactions of metal‐containing nanoparticles with biomolecules by CE: An update (2012–2016)

Analysis and applications of nanoparticles in capillary electrophoresis

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