Detection of c-reactive protein based on a magnetic immunoassay by using functional magnetic and fluorescent nanoparticles in microplates

Yang, Song; Gao, Bao; Tsai, Horng-Jyh; Fuh, C. Bor

doi:10.1039/c4an00921e

Cited by 28 publications

(17 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have also demonstrated that the SLISA can be analysed using SERRS by utilising the roughened surface of the Ag NPs in combination with a laser in resonance with the absorbance maxima of the oxidised TMB. This has allowed for the novel detection of CRP with a calculated detection limit of 1.09 ng/mL., which is comparable to LODs that have been achieved using fluorescence, 25 colorimetric, 26 and electrochemical detection 26 which have reported detection limits of between 1-2 ng/mL. The SLISA also showed good selectivity and the practicality was demonstrated by detecting CRP in a biological matrix.…”

Section: Resultsmentioning

confidence: 54%

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

Sloan-Dennison

Laing

Shand

et al. 2017

Analyst

View full text Add to dashboard Cite

aArtificial enzymes have become an increasingly interesting area of research due to their many advantages over natural protein enzymes which are expensive, difficult to isolate and unable to stand harsh environments. An important area of this research involves using metal nanoparticles as artificial enzymes, known as nanozymes, which exhibit peroxidase-like activity enabling them to catalyse the oxidation of substrates such as 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H2O2), giving a colorimetric response. Here we exploit the catalytic activity of silver nanoparticles (Ag NPs) in a surface based silver-linked immunosorbent assay (SLISA) to detect human C-reactive protein (CRP), an inflammatory marker. Ag NPs were conjugated to antibodies with specific recognition for the corresponding target antigenic molecule, CRP, and the Ag NPs were used to catalyse the oxidation of TMB by H2O2. The resulting coloured oxidation product was detected using SERRS. We demonstrate that Ag NPs can replace the enzymes used in a conventional ELISA and a detection limit of 1.09 ng/mL of CRP can be achieved. It indicates the promise for SLISAs for biomarker detection and opens the way for further assays of this nature to be created. This novel assay has the potential to be optimised to detect lower levels of CRP and can be further extended for the sensitive and specific detection of other relevant biomarkers.

show abstract

Section: Resultsmentioning

confidence: 54%

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

Sloan-Dennison

Laing

Shand

et al. 2017

Analyst

View full text Add to dashboard Cite

show abstract

“…Consequently, the thick shell InP/GaP/ZnS core/shell QDs with high PL QY and high stability are identified as the optimized fluorescent label material in our study. In addition, the analytical performance of this method has been compared with other previously reported immunoanalysis studies for CRP detection . From Table 1 , compared with the Cd‐free QD system immunoassay, the thick shell InP/GaP/ZnS QD‐FLISA shows high sensitivity and broad detection range, which can be comparable to the Cd‐based QD immunoassay.…”

Section: Resultsmentioning

confidence: 97%

“…2020, 37,1900441 for CRP detection. [26,28,[53][54][55][56] From Table 1, compared with the Cd-free QD system immunoassay, the thick shell InP/GaP/ZnS QD-FLISA shows high sensitivity and broad detection range, which can be comparable to the Cd-based QD immunoassay. It indicated that the excellent detection performance of the thick shell InP/GaP/ZnS QD-FLISA have huge potentials for bioassays and other biological medical applications.…”

Section: Detection Performance Of Inp/gap/zns Qd-flisa For Crpmentioning

confidence: 99%

Preparation of Highly Stable and Photoluminescent Cadmium‐Free InP/GaP/ZnS Core/Shell Quantum Dots and Application to Quantitative Immunoassay

et al. 2020

Part & Part Syst Charact

View full text Add to dashboard Cite

Indium phosphide (InP) quantum dots (QDs) are ideal substitutes for widely used cadmium‐based QDs and have great application prospects in biological fields due to their environmentally benign properties and human safety. However, the synthesis of InP core/shell QDs with biocompatibility, high quantum yield (QY), uniform particle size, and high stability is still a challenging subject. Herein, high quality (QY up to 72%) thick shell InP/GaP/ZnS core/shell QDs (12.8 ± 1.4 nm) are synthesized using multiple injections of shell precursor and extension of shell growth time, with GaP serving as the intermediate layer and 1‐octanethiol acting as the new S source. The thick shell InP/GaP/ZnS core/shell QDs still keep high QY and photostability after transfer into water. InP/GaP/ZnS core/shell QDs as fluorescence labels to establish QD‐based fluorescence‐linked immunosorbent assay (QD‐FLISA) for quantitative detection of C‐reactive protein (CRP), and a calibration curve is established between fluorescence intensity and CRP concentrations (range: 1–800 ng mL−1, correlation coefficient: R2 = 0.9992). The limit of detection is 2.9 ng mL−1, which increases twofold compared to previously reported cadmium‐free QD‐based immunoassays. Thus, InP/GaP/ZnS core/shell QDs as a great promise fluorescence labeling material, provide a new route for cadmium‐free sensitive and specific immunoassays in biomedical fields.

show abstract

“…9,10 Although, previous papers have reported success with the sensing system, these methods are usually time-consuming and complicated detection procedures. 9,10 Immunofiltration assays (IFAs) generally rely on the liquid flow technology in which a porous nitrocellulose (NC) membrane is employed as a solid support for immobilizing or capturing the analyte to be detected, along with a proper signal reporting system in a typical sandwich reaction. [11][12][13] Practically, IFAs are very simple, fast, and cost-effective.…”

mentioning

confidence: 99%

Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay

Zhang¹,

Bao²,

Draz³

et al. 2015

IJN

View full text Add to dashboard Cite

Convenient and rapid immunofiltration assays (IFAs) enable on-site “yes” or “no” determination of disease markers. However, traditional IFAs are commonly qualitative or semi-quantitative and are very limited for the efficient testing of samples in field diagnostics. Here, we overcome these limitations by developing a quantum dots (QDs)-based fluorescent IFA for the quantitative detection of C-reactive proteins (CRP). CRP, the well-known diagnostic marker for acute viral and bacterial infections, was used as a model analyte to demonstrate performance and sensitivity of our developed QDs-based IFA. QDs capped with both polyethylene glycol (PEG) and glutathione were used as fluorescent labels for our IFAs. The presence of the surface PEG layer, which reduced the non-specific protein interactions, in conjunction with the inherent optical properties of QDs, resulted in lower background signal, increased sensitivity, and ability to detect CRP down to 0.79 mg/L with only 5 µL serum sample. In addition, the developed assay is simple, fast and can quantitatively detect CRP with a detection limit up to 200 mg/L. Clinical test results of our QD-based IFA are well correlated with the traditional latex enhance immune-agglutination aggregation. The proposed QD-based fluorescent IFA is very promising, and potentially will be adopted for multiplexed immunoassay and in field point-of-care test.

show abstract

Detection of c-reactive protein based on a magnetic immunoassay by using functional magnetic and fluorescent nanoparticles in microplates

Cited by 28 publications

References 14 publications

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

A novel nanozyme assay utilising the catalytic activity of silver nanoparticles and SERRS

Preparation of Highly Stable and Photoluminescent Cadmium‐Free InP/GaP/ZnS Core/Shell Quantum Dots and Application to Quantitative Immunoassay

Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay

Contact Info

Product

Resources

About