2023
DOI: 10.9734/ijpss/2023/v35i102926
|View full text |Cite
|
Sign up to set email alerts
|

Detection of "Candidatus Liberibacter asiaticus" Using Conventional PCR and Nested-PCR and Identification of Primer Sensitivity through Q-PCR in HLB Infected Mandarin Orange and Acid Lime Plants in Tamil Nadu

Abstract: One of the most destructive citrus diseases in the world is called citrus Huanglongbing (HLB). For effective HLB associated ‘Candidatus liberibacter spp.’ prevention, sensitive and precise diagnostics are essential. Candidatus Liberibacter asiaticus (CLas), Candidatus Liberibacter africanus (CLaf), and Candidatus Liberibacter americanus (CLam) are among the ‘Candidatus liberibacter spp.’ that infect citrus. The most common of these is the species known as Las. To assess the impact of citrus greening disease in… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2024
2024
2024
2024

Publication Types

Select...
1

Relationship

0
1

Authors

Journals

citations
Cited by 1 publication
(1 citation statement)
references
References 12 publications
0
1
0
Order By: Relevance
“…Thus, early diagnosis is of vital importance in the fight against HLB . An early method for detecting HLB is field symptom diagnosis, which has a high rate of false positives because the symptoms of citrus HLB are similar to those caused by zinc or other nutrient deficiencies. , Currently, sensitive and accurate molecular detection technologies, such as routine polymerase chain reaction (PCR), nested PCR, and real-time fluorescence quantitative PCR, have been established for the detection of HLB pathogens including 16S rDNA and beta-operon gene. , However, their limited applicability due to the complicated primer constructions, parameter optimizations, and especially the need for precise thermoregulation for specific real samples call for alternative methods. Therefore, development of new sensitive and robust testing tools for better control and eradication of this destructive citrus disease is still an urgent need.…”
Section: Introductionmentioning
confidence: 99%
“…Thus, early diagnosis is of vital importance in the fight against HLB . An early method for detecting HLB is field symptom diagnosis, which has a high rate of false positives because the symptoms of citrus HLB are similar to those caused by zinc or other nutrient deficiencies. , Currently, sensitive and accurate molecular detection technologies, such as routine polymerase chain reaction (PCR), nested PCR, and real-time fluorescence quantitative PCR, have been established for the detection of HLB pathogens including 16S rDNA and beta-operon gene. , However, their limited applicability due to the complicated primer constructions, parameter optimizations, and especially the need for precise thermoregulation for specific real samples call for alternative methods. Therefore, development of new sensitive and robust testing tools for better control and eradication of this destructive citrus disease is still an urgent need.…”
Section: Introductionmentioning
confidence: 99%