Detection of canine parvovirus types 2b and 2c in canine faecal samples contaminating urban thoroughfares in Brazil

Abstract

Canine parvovirus type 2 (CPV-2) is a highly contagious virus that causes acute gastroenteritis in dogs all over the world. Because of its stability in the environment, CPV-2 can remain infective for a long time, especially if protected in organic matter. To demonstrate CPV-2’s potential as an environmental hazard for nonimmunized susceptible hosts, we investigated 50 faecal samples collected from public areas in a municipality of Paraná state, Brazil. Seven samples tested positive for CPV by a PCR assay targeting the partial VP2 gene, with three strains being confirmed as CPV-2b variant and one as CPV-2c variant by sequence analysis. These findings were supported by phylogenetic analysis, and the species identity of faecal samples source was confirmed by canine mitochondrial DNA amplification and sequencing. Our results demonstrate the presence of CPV in canine faeces contaminating urban thoroughfares and reinforce the importance of environmental control to reduce the potential exposure risks to susceptible hosts.

Canine parvovirus 2 detection using a LSPR biosensing method with gold nanoparticles

“…Please do not adjust margins Please do not adjust margins care testing diagnosis as well. Besides AuNPs diagnostics techniques already has been related to fast colorimetric diagnostics protocols, 6,[8][9][10][11][12] the present experiments did not have a colorimetric change after the virus binding on the AuNPs complex, similar to a previous report of Dengue virus detection. 7 However, when the AuNPs were combined with a surface active maghemite nanoparticles and specific aptamers, a colorimetric change were accomplish.…”

“…9 Characterized as an acute infectious disease, faecaloronasal contact is the main transmission route with a significant spreads rates. 1,10,11 After the oronasal contact, the CPV-2 can replicate on cells in the dividing phase, especially on the gastrointestinal and lymphoid tissue. 1 Thus, infecting leucocytes and then the crypt cells of the small intestine leads to shortening and even necrosis.…”

Detection of environmental contamination with feline and canine parvoviruses: new perspectives and challenges

“…The presence of contaminated faeces has also been reported on urban surfaces and in public areas (Gogone et al . 2020). Recurrent outbreaks are described in the environments where previous cases have been reported (Sarpong et al .…”