Detection of canine pemphigus foliaceus autoantigen by immunoblotting

Iwasaki, Tadaaki; Shimizu, Motoko; Obata, Hideo; Isaji, Motomi; Yanai, Tokuma; Kitagawa, Hitoshi; Sasaki, Yoshihide

doi:10.1016/s0165-2427(97)00054-8

Cited by 28 publications

(35 citation statements)

References 17 publications

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“…[5][6][7]13 The PRRSV is a member of the genus Arterivirus, family Arteriviridae, order Nidovirales, and has a positive single-stranded polyadenylated RNA molecule ap- proximately 15 kb in length that contains 8 open reading frames (ORFs). 3,20 There are 2 distinct genotypes of PRRSV: the North American and European genotypes.…”

Section: Detection and Differentiation Of North American And Europeanmentioning

confidence: 99%

“…9 The target antigen is a desmosomal transmembrane glycoprotein, desmoglein 1, of the cadherin family of intercellular adhesion molecules. 6,11 The diagnosis of pemphigus foliaceus in dogs relies on multiple criteria including clinical signs, distribution of lesions, histopathology, and immunohistochemistry. Immunohistochemical staining for immunoglobulin and complement components may not distinguish pemphigus foliaceus from superficial pyoderma associated with bacterial infections, as similar deposition patterns may occur.…”

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confidence: 99%

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Altered Immunohistochemical Staining for Desmoglein in Skin Biopsies in Canine Pemphigus Foliaceus

et al. 2002

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Abstract. In this study, 50 cases of canine pemphigus foliaceus and 49 cases of canine superficial pyoderma were examined by immunohistochemical staining for patterns of desmoglein expression. In 31/50 (62%) of pemphigus foliaceus cases, there was an altered staining pattern for desmoglein consisting of distinct clumped deposits at the periphery of keratinocytes and/or dark cytoplasmic staining of acantholytic cells (consistent with internalization of desmoglein). In contrast, desmoglein staining in biopsies from cases of superficial pyoderma was diffusely pale without evidence for clumping or distinct internalization. This study demonstrates that epidermal desmoglein expression is altered in some cases of pemphigus foliaceus in dogs and suggests that immunohistochemical staining for this protein may be useful in diagnosis.Pemphigus foliaceus is an autoimmune skin disease (AISD) of people and animals characterized by lack of adhesion between keratinocytes (acantholysis) and intraepidermal vesicle or pustule formation. 9 In 77% of dogs with pemphigus foliaceus, antikeratinocyte antibodies can be demonstrated. 9 The target antigen is a desmosomal transmembrane glycoprotein, desmoglein 1, of the cadherin family of intercellular adhesion molecules. 6,11 The diagnosis of pemphigus foliaceus in dogs relies on multiple criteria including clinical signs, distribution of lesions, histopathology, and immunohistochemistry. Immunohistochemical staining for immunoglobulin and complement components may not distinguish pemphigus foliaceus from superficial pyoderma associated with bacterial infections, as similar deposition patterns may occur. 8,9 Immunohistochemical staining for desmoglein 1 has been used to demonstrate an altered pattern of desmoglein expression in lesional and perilesional skin from people with pemphigus foliaceus. In skin from healthy individuals, desmoglein 1 occurs in an evenly distributed, finely stippled pattern at the periphery of keratinocytes. [2][3][4]12 In contrast, both lesional and nonlesional skin from pemphigus foliaceus patients may have patchy pericytoplasmic staining coupled with cytoplasmic staining of acantholytic cells. [2][3][4] From the Departments of Veterinary Pathology (Steeves, Clark) and Veterinary Microbiology (Chelak, Haines), Western College of Veterinary Medicine, 52 Campus Drive, University of Saskatchewan,

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Section: Detection and Differentiation Of North American And Europeanmentioning

confidence: 99%

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confidence: 99%

Altered Immunohistochemical Staining for Desmoglein in Skin Biopsies in Canine Pemphigus Foliaceus

et al. 2002

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“…Recent paper described that injection of serum IgG from three dogs with PF to neonatal mice caused acantholytic blisters at the superficial layer of epidermis [12]. Early immunoblotting studies have revealed that serum IgG found in dogs with PF recognizes a 148-kDa or 160-kDa antigen in canine keratinocyte extracts, which is presumed to be a canine homologue of human Dsg1 [10,17]. On the other hand, a recent paper has demonstrated that serum IgG against the extracellular region of canine Dsg1 was recognized only in some dogs with PF [13].…”

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confidence: 99%

Neutrophils Contact to Plasma Membrane of Keratinocytes Including Desmosomal Structures in Canine Pemphigus Foliaceus

Yabuzoe

Nishifuji

Sekiguchi

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Pemphigus foliaceus (PF) is an autoimmune blistering skin disease that affects certain mammals including dogs. In canine PF, neutrophils are infiltrated intensely into pustular lesions including acantholytic cells, although neutrophilic infiltration is not characterized in human PF. The roles of the neutrophils in the cutaneous lesions of canine PF have not yet been understood. The purpose of this study was to characterize the ultrastructural features underlying the acantholysis with pustule formation in canine PF. Four dogs diagnosed as PF on the basis of clinical signs, histopathological findings, and direct and indirect immunofluorescence examinations were performed. Electron microscopy revealed that the acantholytic cells were adjacent to multiple neutrophils in the pustules. At the contact points between neutrophils and acantholytic keratinocytes, half-desmosomes of acantholytic keratinocytes with intact attachment plaques were observed within invaginations of neutrophils. Furthermore, on the surface of acantholytic cells in the pustules, neutrophil granules seemed to be secreted to the surface of acantholytic cells and to degenerate the half-desmosome structures. Neutrophils were also observed within the epidermis adjacent to the pustule. At the intercellular gap between two dissociated keratinocytes, neutrophils inserted its pseudopodia into the gap between the two half-desmosomes of keratinocytes. These findings taken together suggested that, at least in the areas where we analyzed ultrastructurally, neutrophils contact desmosomal structures and seem to play some parts in separation of keratinocytes and degeneration of split-desmosomes in pustules of dogs with PF.

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“…Serum collected at initial presentation was available from Cases 2 and 4 and was tested for the presence of immunoreactivity (autoantibodies) against the surface of living canine keratinocytes (IgG, IgM, IgA and C3) 4 . No antibodies were detected in either dog’s sera.…”

Section: Immunological Studiesmentioning

confidence: 99%

“…Canine pemphigus foliaceus is an autoimmune antibody‐mediated skin disease whose aetiology is unknown, although a genetic predisposition in some breeds has been noted 1–3 . Circulating autoantibodies have been recognized in dogs with pemphigus foliaceus, and may share at least one of the same target proteins (desmoglein 1) as in the disease in humans 4 . Pemphigus foliaceus caused by the administration of certain drugs has been a well‐recognized disease entity in humans but reports in dogs are rare 5–7 .…”

Section: Introductionmentioning

confidence: 99%

Putative drug‐related pemphigus foliaceus in four dogs

White

Carlotti

et al. 2002

Veterinary Dermatology

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Four dogs developed cutaneous lesions following the administration of various antibiotics. Histopathology of the lesions was compatible with pemphigus foliaceus, although apoptotic cells suggestive of erythema multiforme were seen in two cases. In two dogs the lesions resolved after 7.5-8.5 months of immune-suppressive treatment. No recurrence was seen during the follow-up period (3 and 4.5 years). The lesions in the other two dogs resolved within 3 weeks to 3 months following discontinuation of the antibiotic. No recurrence of clinical signs occurred during the follow-up period (1 and 4 years, respectively).

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Detection of canine pemphigus foliaceus autoantigen by immunoblotting

Cited by 28 publications

References 17 publications

Altered Immunohistochemical Staining for Desmoglein in Skin Biopsies in Canine Pemphigus Foliaceus

Altered Immunohistochemical Staining for Desmoglein in Skin Biopsies in Canine Pemphigus Foliaceus

Neutrophils Contact to Plasma Membrane of Keratinocytes Including Desmosomal Structures in Canine Pemphigus Foliaceus

Putative drug‐related pemphigus foliaceus in four dogs

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