Detection of Capnocytophaga canimorsus and Capnocytophaga cynodegmi by Cultural and Molecular Methods in Dogs in Western Turkey

Abstract

The aim of this study is to exhibit the presence of Capnocytophaga species in Turkey and to determine the regional risks of these bacteria for the people in dogs. Totally 200 oral swab samples were taken from owned dogs which have had dental plaque problems. The diagnosis of Capnocytophaga infections were done by PCR using CaL2, AS1, CaR and CyR gene sequence primers. The first PCR was carried out to samples using by CaL2 and AS1 primers and results were estimated as Capnocytophaga species. The second PCR was applied to samples using CaR and CyR reverse primers and results were also interpreted as being Capnocytophaga canimorsus and Capnocytophaga cynodegmi. At the end of the first PCR, Capnocytophaga spp. was detected from 11 (5.5%) out of 200 samples. CaR and CyR gene were investigated in samples which were detected as Capnocytophaga spp. It was determined that 2 (18.2%) of the samples were positive for CaR gene which were identified as C. canimorsus and 9 (81.8%) of the samples were positive for both CaR and CyR gene which were identified as C. canimorsus and C. cynodegmi. C. canimorsus and C. cynodegmi species are concluded for generating risk for human health and due to the lacking of information about the disease it was also difficult to diagnosis these agents.

Halk Sağlığı Açısından Pet ve Başıboş Kedilerde Bartonella henselae Prevelansı: Tek Tıp - Tek Sağlık Konseptinde Örnek Bir Çalışma

“…One of the primary goals of the One Health concept is to advance the understanding, prevention, and treatment of zoonotic disease [1] . CSD, known since the 1950s, is an important zoonosis, caused by Bartonella henselae [2][3][4][5] . Cats act as reservoirs in the transmission of Bartonella henselae to humans [2][3][4] .…”

“…Cats act as reservoirs in the transmission of Bartonella henselae to humans [2][3][4] . Spreading of Bartonella henselae from cats to humans is either directly by cat scratch and bite or indirectly by cat fleas and flea excrement [4][5][6] . Cat fleas harbour Bartonella henselae in their intestines, spread it in the environment via faeces and transmit the infection among cats.…”