1996
DOI: 10.1016/0378-1135(96)00063-6
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Detection of caprine arthritis-encephalitis virus RNA in macrophages by in situ hybridization using fluorescein-labelled single-stranded RNA probes

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“…Similarly, immunohistochemical methods, allowing the detection of viral protein antigens by specific antibodies in histological samples, smears or cytospin preparations, are useful in research and confirmatory studies, but are not commonly applied in differential routine diagnosis on live animals, because of the cost, low availability of reagents and samples and limited sensitivity of some of these techniques [175]. Also, in situ hybridization methods have been employed for histologic studies, but this laborious procedure is only used for research purposes [176,177]. Although PCR-based diagnosis is the most common of the molecular techniques applied in SRLV diagnosis in vitro (see below), indirect methods such as serological diagnosis to detect antibodies to SRLV are overall the most commonly applied approach to detect infection.…”
Section: In Vitro Diagnosismentioning
confidence: 99%
“…Similarly, immunohistochemical methods, allowing the detection of viral protein antigens by specific antibodies in histological samples, smears or cytospin preparations, are useful in research and confirmatory studies, but are not commonly applied in differential routine diagnosis on live animals, because of the cost, low availability of reagents and samples and limited sensitivity of some of these techniques [175]. Also, in situ hybridization methods have been employed for histologic studies, but this laborious procedure is only used for research purposes [176,177]. Although PCR-based diagnosis is the most common of the molecular techniques applied in SRLV diagnosis in vitro (see below), indirect methods such as serological diagnosis to detect antibodies to SRLV are overall the most commonly applied approach to detect infection.…”
Section: In Vitro Diagnosismentioning
confidence: 99%