Detection of carbamic and organophosphorous pesticides in water samples using a cholinesterase biosensor based on Prussian Blue-modified screen-printed electrode

Arduini, Fabiana; Ricci, Francesco; Tuță, Cătălin Stelian; Moscone, Danila; Amine, Aziz; Palleschi, Giuseppe

doi:10.1016/j.aca.2006.07.052

Cited by 230 publications

(97 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[1,2] The presence of pesticide residues and metabolites in food, water and soil currently represents one of the major issues for environmental chemistry. Pesticides are among the most important environmental pollutants because of their increasing use in agriculture.…”

Section: Introductionmentioning

confidence: 99%

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Chauhan

Narang

Jain

2015

Journal of Experimental Nanoscience

View full text Add to dashboard Cite

A new electrochemical sensing device was constructed for determination of pesticides. In this report, acetylcholinesterase was bioconjugated onto hybrid nanocomposite, i.e. iron oxide nanoparticles and poly(indole-5-carboxylic acid) (Fe 3 O 4 NPs/Pin5COOH) was deposited electrochemically on glassy carbon electrode. Fe 3 O 4 NPs was showed as an amplified sensing interface at lower voltage which makes the sensor more sensitive and specific. The enzyme inhibition by pesticides was detected within concentrations ranges between 0.1À60 and 1.5À70 nM for malathion and chlorpyrifos, respectively, under optimal experimental conditions (sodium phosphate buffer, pH 7.0 and 25 C). Biosensor determined the pesticides level in water samples (spiked) with satisfactory accuracy (96%À100%). Sensor showed good storage stability and retained 50% of its initial activity within 70 days at 4 C.

show abstract

Section: Introductionmentioning

confidence: 99%

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Chauhan

Narang

Jain

2015

Journal of Experimental Nanoscience

View full text Add to dashboard Cite

show abstract

“…It has been found that both the sensitivity and detection accuracy decrease with the increase in the concentration of the pesticides. A comparative study using Co-phthalocyanine and Prussian Blue-modified screenprinted electrodes has also been performed (Arduini et al 2006). The limit of detection for Co-phthalocyanine and Prussian blue is LOD of 5×10 −7 and 5×10 −6 M, respectively with high potentialities for pesticide measurement.…”

Section: Detection Of Pesticide Residuesmentioning

confidence: 99%

Optical biosensors for food quality and safety assurance—a review

et al. 2011

View full text Add to dashboard Cite

Food quality and safety is a scientific discipline describing handling, preparation and storage of food in ways that prevent food borne illness. Food serves as a growth medium for microorganisms that can be pathogenic or cause food spoilage. Therefore, it is imperative to have stringent laws and standards for the preparation, packaging and transportation of food. The conventional methods for detection of food contamination based on culturing, colony counting, chromatography and immunoassay are tedious and time consuming while biosensors have overcome some of these disadvantages. There is growing interest in biosensors due to high specificity, convenience and quick response. Optical biosensors show greater potential for the detection of pathogens, pesticide and drug residues, hygiene monitoring, heavy metals and other toxic substances in the food to check whether it is safe for consumption or not. This review focuses on optical biosensors, the recent developments in the associated instrumentation with emphasis on fiber optic and surface plasmon resonance (SPR) based biosensors for detecting a range of analytes in food samples, the major advantages and challenges associated with optical biosensors. It also briefly covers the different methods employed for the immobilization of bio-molecules used in developing biosensors.

show abstract

“…The amperometric detection of cholinesterase activity is mainly based on direct or mediated oxidation of thiocholine, the enzymatic product of thiocholine ester. Similar biosensing systems have been widely described in literature for the detection of paraoxon [13,14], chlorpyrifos [15,16], malaoxon [17,18], and dichlorvos [19,20].…”

Section: Introductionmentioning

confidence: 99%

“…In order to fine-tune an analytical system capable to properly work in waste water samples, without sophisticated sample pre-treatment and interference problems, the "medium exchange method", we proposed in a previous work, was adopted [15]. This method consists in three steps.…”

Section: Organophosphate Flow Analysismentioning

confidence: 99%

Automatable Flow System for Paraoxon Detection with an Embedded Screen-Printed Electrode Tailored with Butyrylcholinesterase and Prussian Blue Nanoparticles

et al. 2015

Self Cite

View full text Add to dashboard Cite

Nowadays extensive volumes of pesticides are employed for agricultural and environmental practices, but they have negative effects on human health. The levels of pesticides are necessarily restricted by international regulatory agencies, thus rapid, cost-effective and in-field analysis of pesticides is an important issue. In the present work, we propose a butyrylcholinesterase (BChE)-based biosensor embedded in a flow system for organophosphorus pesticide detection. The BChE was immobilized by cross-linking on a screen-printed electrode modified with Prussian Blue Nanoparticles. The detection of paraoxon (an organophosphorus pesticide) was carried out evaluating its inhibitory effect on BChE, and quantifying the enzymatic hydrolysis of butyrylthiocholine before and after the exposure of the biosensor to paraoxon, by measuring the thiocholine product at a working voltage of +200 mV. The operating conditions of the flow system were optimized. A flow rate of 0.25 mL/min was exploited for inhibition steps, while a 0.12 mL/min flow rate was used for substrate measurement. A substrate concentration of 5 mM and an incubation time of 10 min allowed a detection limit of 1 ppb of paraoxon (corresponding to 10% inhibition). OPEN ACCESSChemosensors 2015, 3 130The stability of the probe in working conditions was investigated for at least eight measurements, and the storage stability was evaluated up to 60 days at room temperature in dry condition. The analytical system was then challenged in drinking, river and lake water samples. Matrix effect was minimized by using a dilution step (1:4 v/v) in flow analysis. This biosensor, embedded in a flow system, showed the possibility to detect paraoxon at ppb level using an automatable and cost-effective bioanalytical system.

show abstract

Detection of carbamic and organophosphorous pesticides in water samples using a cholinesterase biosensor based on Prussian Blue-modified screen-printed electrode

Cited by 230 publications

References 41 publications

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Optical biosensors for food quality and safety assurance—a review

Automatable Flow System for Paraoxon Detection with an Embedded Screen-Printed Electrode Tailored with Butyrylcholinesterase and Prussian Blue Nanoparticles

Contact Info

Product

Resources

About