Detection of Carboxylesterase 1 and Chlorpyrifos with ZIF-8 Metal–Organic Frameworks Using a Red Emission BODIPY-Based Probe

Abstract

In this work, a red emission fluorescent probe CBZ-BOD@ zeolitic imidazolate framework-8 (ZIF-8) was fabricated based on metal− organic frameworks (MOFs) for detecting carboxylesterase 1 (CES1). The small molecule probe CBZ-BOD was first synthesized and then used to prepare the functionalized MOF material. ZIF-8 was chosen as an encapsulation shell to improve the detection properties of CBZ-BOD. Using this unique porous materials, ultrasensitive quantification of CES1 and chlorpyrifos was successfully realized. The low detection limit and high fluorescence quantum yield were calculated as 1.15 ng/mL and 0.65 for CBZ-

Fabricating Bimetal Organic Material Capsules with a Commodious Microenvironment and Synergistic Effect for Glycosyltransferase

“…PmHS2 was successfully expressed in PmHS2-E. coli BL21-(DE3) engineered bacteria and purified by nickel affinity chromatography (Figure S1). Then, using disaccharide GlcNAc-α1→4-GlcA-pNP synthesis as a reaction model, we investigated four common MOF materials to immobilize PmHS2 on the basis of our previous work 26,27 (Table 1 and Figures S2−S7). However, the zeolitic imidazolate frameworks (ZIF)-8 and ZIF-67, the most widely used hydrophobic MOFs for enzyme immobilization, are not adapted for the catalysis of PmHS2 (PmHS2@ZIF-8/ZIF-67, Table 1, entries 1 and 2).…”

In situ encapsulation of ZrQ in UiO‐66 (Zr‐BDC) for pore size control to enhance detection of a nerve agent simulant dimethyl methyl phosphonate

“…[8] Metal organic frameworks (MOFs) have shown promise for numerous sensing applications. [9][10][11][12] Fluorescent sensors have been synthesized using Zr, 4,4 0stilbenedicarboxylic acid and Eosin Y for nitroaromatics sensing. [13] Zr-BDC, also known as UiO-66, have been used for DMMP detection and even degradation.…”

CRISPR/Cas12a-mediated electrochemiluminescence platform for environmental and human serum SARS-CoV-2 RNA monitoring using a self-enhanced ruthenium complex linked to zeolitic imidazole framework-8

“…28 In addition, it was widely utilized in the loading of nanomaterials because it can prevent the aggregation of nanomaterials and improve the distribution of nanomaterials to enhance the optical, electrical and catalytic properties. 29,30 Inspired by these characteristics, ZIF-8 as a carrier to wrap the intramolecular complex of ECL-emitting reagents with PEI will be beneficial to improve the distribution of ECL emitters and enhance the ECL intensity and emitting efficiency of a bioanalysis system. 31…”

“…28 In addition, it was widely utilized in the loading of nanomaterials because it can prevent the Environmental Science: Nano Paper aggregation of nanomaterials and improve the distribution of nanomaterials to enhance the optical, electrical and catalytic properties. 29,30 Inspired by these characteristics, ZIF-8 as a carrier to wrap the intramolecular complex of ECL-emitting reagents with PEI will be beneficial to improve the distribution of ECL emitters and enhance the ECL intensity and emitting efficiency of a bioanalysis system. 31 Isothermal amplification methods including nucleaseassisted target recycling amplification, rolling circle amplification, and loop-mediated isothermal amplification have been widely employed in biochemical analysis.…”