Detection of cardiovascular disease associated miR-29a using paper-based microfluidics and surface enhanced Raman scattering

Mabbott, Samuel; Fernandes, Syrena C.; Schechinger, Monika; Coté, Gerard L.; Faulds, Karen; Mace, Charles R.; Graham, Duncan

doi:10.1039/c9an01748h

Cited by 50 publications

(32 citation statements)

References 43 publications

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“…To avoid any Raman signal background for the test lines, the LF strips were then washed by dipping into 0.01 M Tris buffer (pH 8.4) for 5 minutes, then allowed to dry at room temperature. Strips were then fixed onto an in-house 3D printed Raman aperture adaptor that was used as a sample holder 35 and the test lines were scanned using a handheld Raman spectrometer (CBEx, Snowy Range Instruments, USA). All the SERS measurements were carried out using orbital raster scanning (ORS) mode over the range 600-1800 cm -1 using the Peak 1.3.68 software.…”

Section: Sers Calibration Curves For Slpa and Toxbmentioning

confidence: 99%

Rapid ultra-sensitive diagnosis of clostridium difficile infection using a SERS-based lateral flow assay

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Johnson

et al. 2021

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Section: Sers Calibration Curves For Slpa and Toxbmentioning

confidence: 99%

Rapid ultra-sensitive diagnosis of clostridium difficile infection using a SERS-based lateral flow assay

Hassanain

Spoors

Johnson

et al. 2021

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“…DEP force was employed to improve the performance of mixing and preconcentration. In another case, paper-based microfluidic SERS system was applied for point-of-care test of microRNA [120]. Driven by capillary action, this system can realize multistep SERS-active nanoparticle conjugation, target microRNA capture, washing, and SERS detection automatically.…”

Section: Multistep Biomacromolecule and Cell Surface-enhanced Raman Spectroscopy Analysismentioning

confidence: 99%

Recent progress of microfluidics in surface‐enhanced Raman spectroscopic analysis

Xia

2021

J of Separation Science

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Surface-enhanced Raman spectroscopy is a significant analytical tool capable of fingerprint identification of molecule in a rapid and ultrasensitive manner. However, it is still hard to meet the requirements of practical sample analysis.The introduction of microfluidics can effectively enhance the performance of surface-enhanced Raman spectroscopy in complex sample analysis including reproducibility, selectivity, sensitivity, and speed. This review summarizes the recent progress of microfluidics in surface-enhanced Raman spectroscopic analysis through four combination approaches. First, microfluidic synthetic techniques offer uniform nano-/microparticle fabrication approaches for reproductive surface-enhanced Raman spectroscopic analysis. Second, the integration of microchip and surface-enhanced Raman spectroscopic substrate provides advanced devices for sensitive and efficient detection. Third, microfluidic sample preparations enable rapid separation and preconcentration of analyte prior to surface-enhanced Raman spectroscopic detection. Fourth, highly integrated microfluidic devices can be employed to realize multistep surface-enhanced Raman spectroscopic analysis containing material fabrication, sample preparation, and detection processes. Furthermore, the challenges and outlooks of the application of microfluidics in surface-enhanced Raman spectroscopic analysis are discussed.

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“…For that reason, researchers have utilized and combined different methods to increase sensitivity, accuracy, and reproducibility of the results. To make POCT and on-field monitoring more affordable, interestingly, supporting devices gradually have been scaled down to minimal but still in well-functioned forms such as printed heater [ 182 ], portable fluorometer [ 195 ], mobile power supply [ 196 ], handheld Raman spectrometer [ 197 ], etc. This is indeed a very dramatic transformation that triggers the competition between manufacturers and allows small enterprises to enter the market, thus opening up the opportunity of commercialization of µPADs.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening

et al. 2020

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Microfluidic paper-based analytical devices (µPADs) have become promising tools offering various analytical applications for chemical and biological assays at the point-of-care (POC). Compared to traditional microfluidic devices, µPADs offer notable advantages; they are cost-effective, easily fabricated, disposable, and portable. Because of our better understanding and advanced engineering of µPADs, multistep assays, high detection sensitivity, and rapid result readout have become possible, and recently developed µPADs have gained extensive interest in parallel analyses to detect biomarkers of interest. In this review, we focus on recent developments in order to achieve µPADs with high-throughput capability. We discuss existing fabrication techniques and designs, and we introduce and discuss current detection methods and their applications to multiplexed detection assays in relation to clinical diagnosis, drug analysis and screening, environmental monitoring, and food and beverage quality control. A summary with future perspectives for µPADs is also presented.

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Detection of cardiovascular disease associated miR-29a using paper-based microfluidics and surface enhanced Raman scattering

Abstract: A paper-based microfluidics self-testing device capable of colorimetric and SERS-based sensing of cardiovascular disease associated miR-29a has been developed for improving patient care and triage.

Cited by 50 publications

References 43 publications

Rapid ultra-sensitive diagnosis of clostridium difficile infection using a SERS-based lateral flow assay

Rapid ultra-sensitive diagnosis of clostridium difficile infection using a SERS-based lateral flow assay

Recent progress of microfluidics in surface‐enhanced Raman spectroscopic analysis

Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening

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