Detection of catechol-O-methyltransferase (COMT) Val158Met Polymorphism by a New Optimized PCR-RFLP Method

Lajin, Bassam; Alachkar, Amal

doi:10.5099/aj110300170

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…A to G substitution in a 5‐HTTLPR variant containing insertion was recognized by the restriction enzyme MspI (New England Biolab, according to Wendland, Martin, Kruse, Lesch, & Murphy, ). The Val and Met alleles of COMT polymorphism were discriminated by the restriction enzyme NlaIII (New England Biolab; Lajin & Alachkar, ). Restriction was followed by agarose gel electrophoresis and visualization by ethidium bromide staining.…”

Section: Methodsmentioning

confidence: 99%

The relationships among MAOA, COMT Val158Met, and 5‐HTTLPR polymorphisms, newborn stress reactivity, and infant temperament

Bajgarová

Bajgar

2019

Brain and Behavior

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Introduction Variance in hypothalamic–pituitary–adrenal (HPA) axis reactivity is considered to be one of the sources of differences in infant temperament. The cortisol enters into interactions with dopamine and serotonin, so it is expected that polymorphisms in genes coding monoamine metabolism influence both HPA axis reactivity and temperament. Methods We therefore explore the relationship among 5‐HTTLPR S/L, MAOA H/L, and COMT Val158Met polymorphisms, the stress reaction of newborn infants after a heel stick blood draw (measured by determining salivary cortisol at three time points), and temperament assessed at the age of 3 months using Rothbart's Infant Behavior Questionnaire—Revised (IBQ‐R) with a sample of 84 infants. Results The decrease in the salivary cortisol correlated with nine primary scales and all three secondary scales of IBQ‐R. Children with a greater cortisol decrease were assessed as less susceptible to negative emotions, more extraverted, and more regulated. The polymorphisms that were observed were related both to the course of the stress reaction and to temperament. The 5‐HTTLPR S allele was connected to higher scores for Negative Emotionality and lower scores for Orienting/Regulatory Capacity. The presence of the MAOA L allele predisposed its carriers to higher scores for Negative Emotionality, lower scores for Orienting/Regulatory Capacity, and a lower decrease in cortisol. The Met allele of COMT Val158Met polymorphism was connected to a higher Positive Affectivity/Surgency and Orienting/Regulatory Capacity and a greater cortisol decrease. Conclusions Contrary to previous studies referring mainly basal cortisol and its increase, the results of our study emphasize the importance of cortisol elimination in infant temperament. Another interesting finding was a higher cortisol increase, higher Distress to Limitations, Negative Emotionality, and Approach in MAOA LL homozygotes which are traditionally understood as more vulnerable toward early stress in developing later externalizing behavior.

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Section: Methodsmentioning

confidence: 99%

The relationships among MAOA, COMT Val158Met, and 5‐HTTLPR polymorphisms, newborn stress reactivity, and infant temperament

Bajgarová

Bajgar

2019

Brain and Behavior

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Genetic Link Between Gender Dysphoria and Sex Hormone Signaling

Foreman

Hare

York

et al. 2018

The Journal of Clinical Endocrinology &Amp; Metabolism

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Pain Candidate Genes 5-HTTLPR and COMT Affect Anxiety and Mood in Japanese Ballet Dancers: A Cross-Sectional and Longitudinal Study

Yatabe,

Ashikaga,

Muroi

et al. 2024

Sports

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The balance of mental, physical, and technical aspects is essential in improving ballet performance. Ballet dancers’ emotional and behavioral characteristics vary, even under identical stress conditions. This study aimed to investigate the association between the pain candidate genes 5-HTTLPR and COMT and anxiety in Japanese ballet dancers. Participants were 18 youth elite ballet students with professional aspirations (Y-Elite) and 16 dancers in a professional ballet company (Pro). We administered psychological questionnaires, the State-Trait Anxiety Inventory (STAI) and Brunel Mood Scale (BRUMS), to participants under the following four different stress conditions: standard practice day, cast decision day, rehearsal day, and one week before competition day. In addition, the genotypes of 5-HTTLPR and COMT Val158Met were examined. The distribution of 5-HTTLPR was not different between Y-Elite and Pro dancers, although one of the COMT genotypes was different. Y-Elite dancers had higher trait anxiety scores than Pro dancers for these genotypes before competition (ps < 0.03), although no significant association was observed between both genotypes and scores on the STAI across conditions. Their moods were significantly different through the four conditions (p < 0.004). Pro dancers’ moods were also more stable than those of the Y-Elite dancers in the presence of pain. The results indicate that 5-HTTLPR and COMT play a crucial role in dancers’ anxiety and mood during pain (ps < 0.05). Pro dancers are probably predicted by their lower neuroticism and mood scores and their better adaptation to stress than Y-Elite dancers. The 5-HTTLPR and COMT genes may be influencing the sensitivity to the environment. Youth elite ballet dancers need to understand the relationship between pain and physical activity from an early stage.

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Detection of catechol-O-methyltransferase (COMT) Val158Met Polymorphism by a New Optimized PCR-RFLP Method

Cited by 3 publications

References 24 publications

The relationships among MAOA, COMT Val158Met, and 5‐HTTLPR polymorphisms, newborn stress reactivity, and infant temperament

The relationships among MAOA, COMT Val158Met, and 5‐HTTLPR polymorphisms, newborn stress reactivity, and infant temperament

Genetic Link Between Gender Dysphoria and Sex Hormone Signaling

Pain Candidate Genes 5-HTTLPR and COMT Affect Anxiety and Mood in Japanese Ballet Dancers: A Cross-Sectional and Longitudinal Study

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