Detection of CD5 in B-cell chronic lymphoproliferative diseases by flow cytometry: a strong expression in B-cell chronic lymphocytic leukemia

Abstract

Cavalcanti Júnior GB, Sales VSF, Silva DGKC, Lopes MCA, Paiva AS, Fonseca HEM, Nascimento Júnior FF, Fernandes MZ. Detection of CD5 in B-cell chronic lymphoproliferative diseases by flow cytometry: a strong expression in B-cell chronic lymphocytic leukemia. Acta Cir Bras [serial on line] Available from: URL: htt://www.scielo.br/acb. ABSTRACT -Purpose:CD5 is a T cell marker, aberrantly express in B cell chronic lymphocytic leukemia (B-CLL) and mantle cell lymphoma (MCL). Other chronic B cell malignancies including hairy cell leukemia (HCL) and B cell prolymphocytic leukemia (B-PLL) are CD5 negative or express this antigen in a weak way. In this study, CD5 expression was investigated in leukemic cells from 42 patients with chronic B cell lymphoproliferative disease. Methods: We studied the CD5 expression in leukemic cells from 42 patients with chronic B-cell malignancies by flow cytometry. Demographic features such as age, sex and clinical date were also analyzed. Results: There were 22 males and 20 females. The immunophenotyping showed that 35 cases were B-CLL, 3 B-PLL and HCL and one patient was MCL. CD5 expression was present in all B-CLL and MCL. Low expression of CD5 was observed in one patient with B-PLL and negative in all cases of HCL. Conclusion: Our date demonstrated that CD5 expression can help distinguish among B-CLL from HCL and B-PLL, but is similar expressed in MCL.

Evaluation of Serum Level of CXCL12 in Correlation with Several Clinical and Hematological Parameters in Patients with Chronic Lymphocytic leukemia

“…For the hematological parameters of the patients, the mean Hb level was 11.50±2.49 g/dl (Mean ±SD) and the mean platelet count was 163.85±76.10x 109 /L (Mean ± SD) and absolute lymphocyte count in the peripheral blood was 49.19 ± 49.75 × 109 / L (Mean ± SD) , these results were comparable to the Iraqi studies results (11,20) also by Western study by (Cavalcanti Júnior GB et al 2005). ( 21) Bone marrow involvement by leukemic cells or by other pathology like splenic pooling are the most probable causes of anemia and thrombocytopenia in these patients .…”

Possible prognostic value of nucleolar morphology in pathologic cells of B-chronic lymphocytic leukemia

“…The membrane phenotype of the B-PLL is quite distinct from that of B-CLL The expression of CD5+, CD19+, CD23+and the absence of FMC7 surface markers are routinely used as diagnostic criteria in patients with B-CLL [15,25]. In addition, the absence of expression of FMC7 is one of the most reliable markers that differentiated B-CLL from other B cell neoplasms [25] and, moreover, CD5 negativity can help distinguish between B-CLL and B-PLL [26]. The CD23 antigen negatively correlates with prolymphocyte infiltration of the BM and PB lymphocyte counts [27].…”