Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos

Jankovičová, Jana; Sečová, Petra; Maňásková-Postlerová, Pavla; Šimoník, Ondřej; Frolikova, Michaela; Eva, Carola; Horovská, Ľubica; Michalková, Katarína; Dvořáková-Hortová, Kateřina; Antalíková, Jana

doi:10.1016/j.ijbiomac.2018.11.161

Cited by 13 publications

(18 citation statements)

References 37 publications

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“…Tetraspanin CD9 expressed on mouse eggs is considered as an essential molecule for successful gamete fusion 7-9 as well as tetraspanin CD81 which is also involved in fertilization 10,11 . Our previous studies concerning species-specific traits of CD9 and CD81 in mouse, bull and porcine gametes during their functional maturation and fertilization, suggested the active participation of tetraspanins in these events [11][12][13][14] . With the ability of tetraspanins being part of signalling cascades involving cytoskeleton 5,15,16 they also play a crucial role in the organization of functional membrane protein cluster domains called a tetraspanin web, which were also described in oocyte and recently in sperm.…”

mentioning

confidence: 91%

Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells

Jankovičová

Frolikova

Palenikova

et al. 2020

Sci Rep

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The physiological importance of CD151 tetraspanin is known from somatic cells and its outside-in signalling through integrins was described. In male germ cells, two tetraspanins, CD9 and CD81, are involved in sperm-egg membrane fusion, and similarly to integrins, they occupy characteristic regions. We report here on a newly discovered presence of CD151 in sperm, and present its expression and distribution during spermatogenesis and sperm transition during the acrosome reaction. We traced CD151 gene and protein expression in testicular cell subpopulations, with strong enrichment in spermatogonia and spermatids. The testicular and epididymal localization pattern is designated to the sperm head primary fusion site called the equatorial segment and when compared to the acrosome vesicle status, CD151 was located into the inner acrosomal membrane overlying the nucleus. Moreover, we show CD151 interaction with α6 integrin subunit, which forms a dimer with β4 as a part of cisprotein interactions within sperm prior to gamete fusion. We used mammalian species with distinct sperm morphology and sperm maturation such as mouse and bull and compared the results with human. In conclusion, the delivered findings characterise CD151 as a novel sperm tetraspanin network member and provide knowledge on its physiology in male germ cells.CD151 belongs to the tetraspanin superfamily, these proteins participate in many biological processes such as growth control, intracellular signalling, cell adhesion, migration, motility 1-4 and they also take place in pathogenesis of some human diseases 5,6 . Tetraspanins create a scaffolding multiprotein network in the membrane that anchors other proteins into specific domains with precise functions and moreover, they are indispensable players in the mammalian fertilization process. Tetraspanin CD9 expressed on mouse eggs is considered as an essential molecule for successful gamete fusion 7-9 as well as tetraspanin CD81 which is also involved in fertilization 10,11 . Our previous studies concerning species-specific traits of CD9 and CD81 in mouse, bull and porcine gametes during their functional maturation and fertilization, suggested the active participation of tetraspanins in these events [11][12][13][14] . With the ability of tetraspanins being part of signalling cascades involving cytoskeleton 5,15,16 they also play a crucial role in the organization of functional membrane protein cluster domains called a tetraspanin web, which were also described in oocyte and recently in sperm. The integral part of the tetraspanin web are integrins and the presence of α3β1, α6β1 and α6β4 integrins was shown in sperm [17][18][19][20] . Importantly, in somatic cells, tetraspanin CD151 was found to play a key role in regulating the adhesion strengthening of integrin α6β1 21 . CD151 has previously been linked with human gamete fusion 22 , associates specifically with α3, α6 and α7 integrins 1,3,23 and links α3β1 and α6β1 integrins to other tetraspanins, including CD9 [24][25][26][27] . Based on that knowledge we p...

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mentioning

confidence: 91%

Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells

Jankovičová

Frolikova

Palenikova

et al. 2020

Sci Rep

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“…Downregulation of IHH, ITGB1, and CD9 connected with folliculogenesis or oogenesis was revealed after IVM. IHH (Indian hedgehog signaling molecule), whose expression in the mucous membrane of the murine uterus was caused by the addition of progesterone [93], and its expression was documented in swine GCs [25,60], also the ITGB1 (integrin subunit beta 1) gene associated with the embryogenesis processes [94], and the CD9 gene, whose contribution to fertility is not yet clear, although it is known to be important in gametes [95]. CD9 is an important factor associated with oocyte membrane and microvilli, playing an active part in the fusion of oocyte and sperm [96].…”

Section: Discussionmentioning

confidence: 99%

Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro

Kulus

Kranc

Ješeta

et al. 2020

Genes

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Polyspermia is an adverse phenomenon during mammalian fertilization when more than one sperm fuses with a single oocyte. The egg cell is prepared to prevent polyspermia by, among other ways, producing cortical granules (CGs), which are specialized intracellular structures containing enzymes that aim to harden the zona pellucida and block the fusion of subsequent sperm. This work focused on exploring the expression profile of genes that may be associated with cortical reactions, and evaluated the distribution of CGs in immature oocytes and the peripheral density of CGs in mature oocytes. Oocytes were isolated and then processed for in vitro maturation (IVM). Transcriptomic analysis of genes belonging to five ontological groups has been conducted. Six genes showed increased expression after IVM (ARHGEF2, MAP1B, CXCL12, FN1, DAB2, and SOX9), while the majority of genes decreased expression after IVM. Using CG distribution analysis in immature oocytes, movement towards the cortical zone of the oocyte during meiotic competence acquisition was observed. CGs peripheral density decreased with the rise in meiotic competence during the IVM process. The current results reveal important new insights into the in vitro maturation of oocytes. Our results may serve as a basis for further studies to investigate the cortical reaction of oocytes.

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“…The authors stated that the CD9 layer is predominantly produced in oocytes, while the CD81 layer is predominantly produced in cumulus cells and localized to the ZP [ 148 ]. In cattle, extracellular vesicles were detected outside the TZP within the perivitelline space of oocytes, and communication of cumulus cells and oocytes via these cell-secreted vesicles was suggested [ 136 ]; later, CD9-positive structures resembling transzonal projections were observed in MII bovine oocytes [ 149 ].…”

Section: Tetraspanins In Evs Of the Female And Male Reproductive Smentioning

confidence: 99%

“…The release of CD9-positive vesicles in mice was also observed immediately after gamete fusion, when CD9 leaves the oocyte PM together with other associated proteins, and thus, likely participates in the prevention of polyspermy [ 172 ]. CD9-positive vesicles in the ZP after fertilization were documented in human zygotes [ 173 ] and in the PVS of bovine and porcine embryos [ 149 ]. Additionally, CD81-positive vesicle clusters were observed in the PVS of bovine [ 174 ] and porcine [ 149 ] embryos.…”

Section: Tetraspanins In Evs Of the Female And Male Reproductive Smentioning

confidence: 99%

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Tetraspanins, More than Markers of Extracellular Vesicles in Reproduction

Jankovičová

Sečová

Michalková

et al. 2020

IJMS

142

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The participation of extracellular vesicles in many cellular processes, including reproduction, is unquestionable. Although currently, the tetraspanin proteins found in extracellular vesicles are mostly applied as markers, increasing evidence points to their role in extracellular vesicle biogenesis, cargo selection, cell targeting, and cell uptake under both physiological and pathological conditions. In this review, we bring other insight into the involvement of tetraspanin proteins in extracellular vesicle physiology in mammalian reproduction. We provide knowledge regarding the involvement of extracellular vesicle tetraspanins in these processes in somatic cells. Furthermore, we discuss the future direction towards an understanding of their functions in the tissues and fluids of the mammalian reproductive system in gamete maturation, fertilization, and embryo development; their involvement in mutual cell contact and communication in their complexity.

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Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos

Cited by 13 publications

References 37 publications

Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells

Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells

Cortical Granule Distribution and Expression Pattern of Genes Regulating Cellular Component Size, Morphogenesis, and Potential to Differentiation are Related to Oocyte Developmental Competence and Maturational Capacity In Vivo and In Vitro

Tetraspanins, More than Markers of Extracellular Vesicles in Reproduction

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