Detection of Charges and Molecules with Self-Assembled Nano-Oscillators

Shan, Xiaonan; Fang, Yunxia; Wang, Shaopeng; Guan, Yan; Chen, Hong‐Yuan; Tao, Nongjian

doi:10.1021/nl501805e

Cited by 51 publications

(81 citation statements)

References 46 publications

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“…35,36 The FFT spectrum of each pixel revealed a peak located at 10.6 Hz. By converting the peak amplitude into temperature with 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 9 the calibration curve shown in Figure 2a, we obtained a PTM image with image intensity representing the local temperature ( Figure 2c) (details are in Methods section).…”

Section: Resultsmentioning

confidence: 99%

Imaging Local Heating and Thermal Diffusion of Nanomaterials with Plasmonic Thermal Microscopy

et al. 2015

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Measuring local heat generation and dissipation in nanomaterials is critical for understanding the basic properties and developing applications of nanomaterials, including photothermal therapy and joule heating of nanoelectronics. Several technologies have been developed to probe local temperature distributions in nanomaterials, but a sensitive thermal imaging technology with high temporal and spatial resolution is still lacking. Here, we describe plasmonic thermal microscopy (PTM) to image local heat generation and diffusion from nanostructures in biologically relevant aqueous solutions. We demonstrate that PTM can detect local temperature change as small as 6 mK with temporal resolution of 10 μs and spatial resolution of submicrons (diffraction limit). With PTM, we have successfully imaged photothermal generation from single nanoparticles and graphene pieces, studied spatiotemporal distribution of temperature surrounding a heated nanoparticle, and observed heating at defect sites in graphene. We further show that the PTM images are in quantitative agreement with theoretical simulations based on heat transport theories.

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Section: Resultsmentioning

confidence: 99%

Imaging Local Heating and Thermal Diffusion of Nanomaterials with Plasmonic Thermal Microscopy

et al. 2015

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“…3a shows a few snapshots of the plasmonic image, which reveal large fluctuations in the image contrast of a bacterial cell. The image contrast fluctuations are due to the bacterial cell movement normal to the sensor surface (Z-direction) 60, 61, due to bacterial metabolism. Additionally, PIT has been shown to track the 3D movement of single bacterial cells and sub-cellular organelles in 3D with < 5 nm spatial resolution and <1 millisecond (ms) temporal resolution 61, 62.…”

Section: Future Technologiesmentioning

confidence: 99%

“…The image contrast fluctuations are due to the bacterial cell movement normal to the sensor surface (Z-direction) 60, 61, due to bacterial metabolism. Additionally, PIT has been shown to track the 3D movement of single bacterial cells and sub-cellular organelles in 3D with < 5 nm spatial resolution and <1 millisecond (ms) temporal resolution 61, 62. This unprecedented capability enables fast tracking of the 3D movement of many bacterial cells simultaneously, providing high throughput quantification of AST with single cell detection capability.…”

Section: Future Technologiesmentioning

confidence: 99%

Current and emerging techniques for antibiotic susceptibility tests

et al. 2017

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Infectious diseases caused by bacterial pathogens are a worldwide burden. Serious bacterial infection-related complications, such as sepsis, affect over a million people every year with mortality rates ranging from 30% to 50%. Crucial clinical microbiology laboratory responsibilities associated with patient management and treatment include isolating and identifying the causative bacterium and performing antibiotic susceptibility tests (ASTs), which are labor-intensive, complex, imprecise, and slow (taking days, depending on the growth rate of the pathogen). Considering the life-threatening condition of a septic patient and the increasing prevalence of antibiotic-resistant bacteria in hospitals, rapid and automated diagnostic tools are needed. This review summarizes the existing commercial AST methods and discusses some of the promising emerging AST tools that will empower humans to win the evolutionary war between microbial genes and human wits.

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“…Other technologies, such as surface plasmon resonance (SPR) [6] and quartz crystal microbalance (QCM), [7] can measure molecular binding processes in real time, but they are based on the detection of molecular mass changes, which are difficult to detect phosphorylation that involves a small mass change (ca. [11] To study phosphorylation, we functionalize the nanoparticles with peptides containing an active tyrosine residue (Supporting Information). For the reasons described above, direct monitoring of phosphorylation has been a challenging task.…”

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confidence: 99%

“…The detection principle can be more precisely described by analyzing the equation of motion of the nano-oscillators given by Equation (1): [11] …”

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confidence: 99%

Real‐Time Monitoring of Phosphorylation Kinetics with Self‐Assembled Nano‐oscillators

et al. 2015

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Phosphorylation is a post-translational modification that is involved in many basic cellular processes and diseases, but is difficult to detect in real time with existing technologies. A label-free detection of phosphorylation is reported in real time with self-assembled nano-oscillators. Each nano-oscillator consists of a gold nanoparticle tethered to a gold surface with a molecular linker. When the nanoparticle is charged, the nano-oscillator can be driven into oscillation with an electric field and detected with a plasmonic imaging approach. The nano-oscillators measure charge change associated with phosphorylation of peptides attached onto a single nanoparticle, allowing us to study the dynamic process of phosphorylation in real time without antibodies down to a few molecules, from which Michaelis and catalytic rate constants are determined.

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Detection of Charges and Molecules with Self-Assembled Nano-Oscillators

Cited by 51 publications

References 46 publications

Imaging Local Heating and Thermal Diffusion of Nanomaterials with Plasmonic Thermal Microscopy

Imaging Local Heating and Thermal Diffusion of Nanomaterials with Plasmonic Thermal Microscopy

Current and emerging techniques for antibiotic susceptibility tests

Real‐Time Monitoring of Phosphorylation Kinetics with Self‐Assembled Nano‐oscillators

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