Detection of Chikungunya Virus Circulation Using Sugar-Baited Traps during a Major Outbreak in French Guiana

Girod, Romain; Guidez, Amandine; Carinci, Romuald; Issaly, Jean; Gaborit, Pascal; Ferrero, Emma; Ardillon, Vanessa; Fontaine, Albin; Dusfour, Isabelle; Briolant, Sébastien

doi:10.1371/journal.pntd.0004876

Cited by 32 publications

(27 citation statements)

References 21 publications

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“…This would negate the need for extensive pre-sorting of samples and ensure malaria detection from any primary or secondary species that feeds on the card within the trap. Alternatively, trap-free bait stations containing FTA cards, similar to those set up for arbovirus surveillance 35 , 36 , could be set up in the field to allow mosquito feeding and subsequent detection of malaria. These FTA card approaches benefit from simplicity and preclude the need for extensive training of personnel; they therefore provide a simple, safe and cost-effective addition that could enhance existing surveillance regimes used by vector control programmes in remote areas.…”

Section: Discussionmentioning

confidence: 99%

Detection of malaria sporozoites expelled during mosquito sugar feeding

Brugman

Kristan

Gibbins

et al. 2018

Sci Rep

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Malaria is a severe disease of global importance transmitted by mosquitoes of the genus Anopheles. The ability to rapidly detect the presence of infectious mosquitoes able to transmit malaria is of vital importance for surveillance, control and elimination efforts. Current methods principally rely on large-scale mosquito collections followed by labour-intensive salivary gland dissections or enzyme-linked immunosorbent (ELISA) methods to detect sporozoites. Using forced salivation, we demonstrate here that Anopheles mosquitoes infected with Plasmodium expel sporozoites during sugar feeding. Expelled sporozoites can be detected on two sugar-soaked substrates, cotton wool and Whatman FTA cards, and sporozoite DNA is detectable using real-time PCR. These results demonstrate a simple and rapid methodology for detecting the presence of infectious mosquitoes with sporozoites and highlight potential laboratory applications for investigating mosquito-malaria interactions. Our results indicate that FTA cards could be used as a simple, effective and economical tool in enhancing field surveillance activities for malaria.

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Section: Discussionmentioning

confidence: 99%

Detection of malaria sporozoites expelled during mosquito sugar feeding

Brugman

Kristan

Gibbins

et al. 2018

Sci Rep

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“…In past years, the military health service participated in monitoring the entomologic status of French military bases in sub-Saharan Africa (Gabon, Ivory Coast, Republic of Central Africa, Senegal, Djibouti) and focused on French Guiana [2] , [3] , [4] , [5] , [6] , [7] , [8] . This surveillance included the identification of vectors, the study of behaviour and the evaluation of insecticide resistance and enabled the development or improvement of new tools for vector trapping [9] , [10] , vector identification such as molecular and proteomic methods [11] , [12] , pathogen identification in vectors [13] , [14] or identification of markers of resistance to insecticides [2] , [3] . New tools to map vector distribution by using remote sensing or meteorologic data have also been developed [15] , [16] .…”

Section: Recent Achievements In Vector-borne Diseasesmentioning

confidence: 99%

Contribution of the French army health service in support of expertise and research in infectiology in Africa

Pradines

Rogier²

2018

New Microbes and New Infections

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Historically, infectious diseases have caused more casualties than battle. The French military health service therefore developed a range of research on vector-borne diseases such as malaria and arboviruses, antibiotic resistance, infectious agents that can be used as biological weapons and vaccines. The main objective is to control naturally acquired or provoked infectious diseases and limit their impact on armed forces as well as on civilian populations in France or abroad, particularly in Africa and anywhere French armies may be deployed. The expertise of the military health service teams in manipulating agents requiring high level of biosafety precautions and in organizing and providing medical care in unnatural conditions, including the battlefield, associated with complementarity staff experience (physicians, biologists, epidemiologists, researchers, pharmacists, logisticians), has been used in the management of the Ebola outbreak in Guinea.

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“…Many of these assays are laborious and laboratory-based and require expensive and bulky instruments, making them incompatible with low-resource regions (24). Recently, several novel, advanced point-of-care (POC) diagnostic measures have been developed for detecting mosquito-borne viruses in the field, including honey-baited nucleic acid preservation cards (25, 26), loop-mediated isothermal amplification (LAMP) (27), biosensors (28), and adaptations of near-infrared spectrometry techniques (29). However, these techniques have documented limitations, including cross-reactivity with other flaviviruses or requirements for training on specialized equipment, making the adoption of these new diagnostic tools difficult (30, 31).…”

Section: Introductionmentioning

confidence: 99%

Field-deployable molecular diagnostic platform for arbovirus andWolbachiadetection inAedes aegypti

Rutkowski

Dong

Dimopoulos

2020

Preprint

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26Background: Surveillance of mosquito infection status is critical for planning and deployment 27 of proper mosquito control initiatives. Concurrently, Wolbachia is being widely used as a control 28 method for arboviral transmission. Point-of-care (POC) detection assays are necessary for 29 monitoring the infection prevalence and geographic range of viruses as well as Wolbachia in 30 mosquito vector populations. We therefore assessed the novel qPCR bCUBE molecular 31 diagnostic system as a tool for virus and Wolbachia detection. 32 Results: We developed a reliable, specific, and sensitive diagnostic assay for detecting Zika 33 virus and dengue virus serotype 2 using the real-time qPCR platform bCUBE. With bCUBE-34 based qRT-PCR, both Wolbachia bacterium and virus RNA could be reliably detected in 35 individually infected Ae. aegypti mosquitoes and in pools of 5, 10, or 15 mosquitoes. 36 Conclusions: The portable qPCR bCUBE diagnostic platform is capable of detecting Zika and 37 dengue virus as well as Wolbachia in mosquitoes and therefore has potential as a practical field-38 deployable diagnostic test for vector-borne disease surveillance programs. 39 40 41 42 43 44 45 46 47

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Detection of Chikungunya Virus Circulation Using Sugar-Baited Traps during a Major Outbreak in French Guiana

Cited by 32 publications

References 21 publications

Detection of malaria sporozoites expelled during mosquito sugar feeding

Detection of malaria sporozoites expelled during mosquito sugar feeding

Contribution of the French army health service in support of expertise and research in infectiology in Africa

Field-deployable molecular diagnostic platform for arbovirus andWolbachiadetection inAedes aegypti

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