Detection of Chlamydia trachomatis by the polymerase chain reaction

Griffais, Rémy; Thibon, M

doi:10.1016/0923-2508(89)90047-8

Cited by 68 publications

(28 citation statements)

References 6 publications

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“…As there are meagre published reports on this aspect from India, an attempt was made to determine the presence of intra-articular C. trachomatis DNA in ReA and uSpA patients by sensitive and specific molecular techniques such as snPCR and nPCR targeting chlamydial MOMP and plasmid genes by primers with good sensitivity [11,28,29] to establish the infection in arthritic patients (both symptomatic for urogenital symptoms as well as asymptomatic) having monoarticular or oligoarticular joint pain in their lower extremities. Patients with inflammatory backache and involvement of joints of the hand were also included in the study, and one RA patient was also diagnosed with Chlamydia infection.…”

Section: Discussionmentioning

confidence: 99%

“…The sequences for primer pairs of MOMP [28] and plasmid [11,29] were taken from published literature for the commercial synthesis of primers.…”

Section: Selection Of C Trachomatis Primersmentioning

confidence: 99%

“…Primers that amplify a plasmid sequence of 201 bp [29] were used for the first amplification. PCR was performed in a 25 µL reaction mixture containing 2. minutes and then for 30 seconds for each cycle, annealing was done at 57°C for 1 minute, extension was done at 45 seconds, and final extension was done for 10 minutes.…”

Section: Nested Polymerase Chain Reaction Assaymentioning

confidence: 99%

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Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and undifferentiated spondyloarthropathy

Prabhash

Bhakuni

Rastogi

2014

J Infect Dev Ctries

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Introduction: There is a paucity of information on the frequency of Chlamydia trachomatis-induced reactive arthritis (ReA) and undifferentiated spondyloarthropathy (uSpA) in India. In this study, arthritic patients suffering from ReA, uSpA, and rheumatoid arthritis (RA) were screened to investigate the presence of C. trachomatis infection in the synovial fluid (SF) or serum by molecular and nonmolecular methods. Methodology: A total of 76 arthritic patients with ReA (n = 16) and uSpA (n = 22) composed the study group while those with RA (n = 38) served as controls. The detection of C. trachomatis DNA was done by semi-nested PCR (snPCR) and nested PCR (nPCR) targeting two different genes of C. trachomatis, namely major outer membrane protein and plasmid, respectively. The presence of serum or SF immunoglobulin IgG and IgA antibodies against C. trachomatis was studied by commercial enzyme-linked immunosorbent assay kits. Results: The SF from 9 of 38 (23.6%) patients (5 with ReA and 4 with uSpA) was positive for at least one C. trachomatis DNA by snPCR or nPCR in comparison to RA (1/38 [2.6%]; p value < 0.05). There was no correlation between the snPCR or nPCR and the serological results of patients with ReA or uSpA. Conclusions: As molecular diagnostic techniques established intra-articular C. trachomatis infection among this group of seronegative spondyloarthropathies in India, these findings should be viewed with concern, and snPCR or nPCR should be considered for a more reliable diagnosis.

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Section: Discussionmentioning

confidence: 99%

“…The sequences for primer pairs of MOMP [28] and plasmid [11,29] were taken from published literature for the commercial synthesis of primers.…”

Section: Selection Of C Trachomatis Primersmentioning

confidence: 99%

Section: Nested Polymerase Chain Reaction Assaymentioning

confidence: 99%

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Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and undifferentiated spondyloarthropathy

Prabhash

Bhakuni

Rastogi

2014

J Infect Dev Ctries

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“…Each sample was supplemented with 4 µl triton 10% (v/v) and 4 µl proteinase K (10µg/ml), followed by incubation at 55ºC for 90 minutes and then at 95ºC for 30 minutes. The samples were maintained at -20 ºC, until used [16].…”

Section: Collection Of Materials and Preparation Of The Samplesmentioning

confidence: 99%

<![CDATA[Detection of Chlamydia trachomatis in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR]]>

Santos¹,

Teixeira²,

Vicente³

et al. 2003

Braz J Infect Dis

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Chlamydia trachomatis is now one of the most prevalent bacteria found in classic sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. We examined the prevalence of Chlamydia trachomatis, by polymerase chain reaction (PCR), in 121 sexually active women who sought treatment for STD in the Alfredo da Matta Institute of Dermatology and Venerology and the Institute of Tropical Medicine of Amazonas in Manaus, Brazil. These women were examined by a specific PCR for the chlamydial plasmid, and the nature of the amplicon was determined by restriction analysis and DNA sequencing. The PCR diagnosis revealed a prevalence of 20.7% infected women.

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“…C. trachomatis was detected by the amplification of a 201 bp sequence by PCR, using Taq DNA Polimerase (Platinum, Invitrogen®) and primers CTP1 (TAG TAA CTG CCA CTT CAT CA) and CTP2 (TTC CCC TTG TAA TTC GTT GC) (Fig. 1B) [42]. Amplification parameters consisted in 39 cycles of 1 min at 95˚C, 1 min at 55˚C and 30 s at 72˚C.…”

Section: Detection Of C Trachomatismentioning

confidence: 99%

Screenning of Chlamydia trachomatis Infection among Women Attending Outpatient Clinic of Infertility

Lavorato¹,

Moço²,

Martín³

et al. 2015

OJOG

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Objective: The goal of this study was to determine the prevalence of C. trachomatis in women diagnosed with infertility attending the Outpatient Clinic of Infertility from Botucatu Medical School, UNESP, Brazil. Patients and Methods: This molecular study enrolled a total of 112 women. Among these patients, 62 presented primary infertility while 50 presented secondary infertility. The criteria for eligibility included women who were: reproductive-aged; no prior report of seroconversion for HIV; no antibiotic or vaginal cream used in the preceding 30 days; and abstinence from sexual intercourse for 72 hours before the visit. The women were submitted to a gynecological examination and cervical samples were collected with an endocervical cytobrush for molecular analysis of C. trachomatis. Results: The prevalence of chlamydial infection was 8% with similar prevalence between primary (8.1%) and secondary (8.0%) infertility. Conclusion: Considering the asymptomatic nature of chlamydial infection and its association with tubal factor infertility, there is a pressing need to incorporate the screening of C. trachomatis infection as part of the routine investigation for infertility. The early diagnostic by screening can minimize complications and reduce Public Health costs with Assisted Reproductive Technology.

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Detection of Chlamydia trachomatis by the polymerase chain reaction

Cited by 68 publications

References 6 publications

Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and undifferentiated spondyloarthropathy

Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and undifferentiated spondyloarthropathy

<![CDATA[<b>Detection of <i>Chlamydia trachomatis</i> in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR</b>]]>

Screenning of <i>Chlamydia trachomatis</i> Infection among Women Attending Outpatient Clinic of Infertility

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Detection of Chlamydia trachomatis by the polymerase chain reaction

Cited by 68 publications

References 6 publications

Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and undifferentiated spondyloarthropathy

Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and undifferentiated spondyloarthropathy

<![CDATA[<b>Detection of <i>Chlamydia trachomatis</i> in endocervical smears of sexually active women in Manaus-AM, Brazil, by PCR</b>]]>

Screenning of &lt;i&gt;Chlamydia trachomatis&lt;/i&gt; Infection among Women Attending Outpatient Clinic of Infertility

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Screenning of <i>Chlamydia trachomatis</i> Infection among Women Attending Outpatient Clinic of Infertility