Detection of Chlamydia trachomatis DNA using MagNA Pure DNA extraction and Cobas Amplicor CT/NG amplification

Martino, Sylvie Josiane De; Barbeyrac, Bertille de; Piémont, Y.; Barthel, Cédric; Monteil, H.; Jaulhac, B.

doi:10.1111/j.1469-0691.2006.01437.x

Cited by 9 publications

(11 citation statements)

References 10 publications

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“…There are very few reports of ESBL production by DEC [31-33]. DEC isolates in these studies were found to harbor bla CTX-M [31-33], bla TEM [32,33] or bla PER genes [33]. In Kuwait, children with invasive diarrhea are normally treated with third generation cephalosporins.…”

Section: Discussionmentioning

confidence: 99%

Diarrhoeagenic Escherichia coli are not a significant cause of diarrhoea in hospitalised children in Kuwait

et al. 2009

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BackgroundThe importance of diarrhoeagenic Escherichia coli (DEC) infections in the Arabian Gulf including Kuwait is not known. The prevalence of DEC (enterotoxigenic [ETEC], enteropathogenic [EPEC], enteroinvasive [EIEC], enterohemorrhagic [EHEC] and enteroaggregative [EAEC]) was studied in 537 children ≤ 5 years old hospitalised with acute diarrhoea and 113 matched controls from two hospitals during 2005–07 by PCR assays using E. coli colony pools.ResultsThe prevalence of DEC varied from 0.75% for EHEC to 8.4% for EPEC (mostly atypical variety) in diarrhoeal children with no significant differences compared to that in control children (P values 0.15 to 1.00). Twenty-seven EPEC isolates studied mostly belonged to non-traditional serotypes and possessed β and θ intimin subtypes. A total of 54 DEC isolates from diarrhoeal children and 4 from controls studied for antimicrobial susceptibility showed resistance for older antimicrobials, ampicillin (0 to 100%), tetracycline (33 to 100%) and trimethoprim (22.2 to 100%); 43.1% of the isolates were multidrug-resistant (resistant to 3 or more agents). Six (10.4%) DEC isolates produced extended spectrum β-lactamases and possessed genetic elements (blaCTX-M, blaTEM and ISEcp1) associated with them.ConclusionWe speculate that the lack of significant association of DEC with diarrhoea in children in Kuwait compared to countries surrounding the Arabian Gulf Region may be attributable to high environmental and food hygiene due to high disposable income in Kuwait.

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Section: Discussionmentioning

confidence: 99%

Diarrhoeagenic Escherichia coli are not a significant cause of diarrhoea in hospitalised children in Kuwait

et al. 2009

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“…However, these calculations are not ideal because the method used for comparison (LightMix) only detects the omp1 gene. Furthermore, the CE Mark-certified protocol was slightly modified; however, these modifications were previously evaluated and may even represent optimisations 7 – 12. Three CTM CT v2.0 low positive samples were not confirmed using BD ProbeTec ET or the new Abbott m 2000rt CT II assay.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the new COBAS TaqMan CT test v2.0 and impact on the proportion of new variant Chlamydia trachomatis by the introduction of diagnostics detecting new variant C trachomatis in Orebro county, Sweden

Hadad

Fredlund

Unemo

2008

Sexually Transmitted Infections

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CTM CT v2.0 is a sensitive and specific method for C trachomatis detection. Studies including larger numbers of symptomatic and asymptomatic patients as well as genital and extragenital samples, and in comparison with other internationally validated and, ideally, US Food and Drug Administration-approved C trachomatis nucleic acid amplification tests are imperative. The proportion of nvCT remains high in Orebro county, Sweden, despite the introduction of genetic diagnostics to detect the mutant.

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“…Clinical samples were extracted by using the automated MagNA Pure DNA extraction (Roche Diagnostics, Meylan, France) [14] then analysed using a Chlamydiaceae-specific real-time PCR targeting the 23S rRNA gene [15] and a specific incA real-time PCR protocol [16].…”

Section: Direct Detection Of Chlamydia Psittaci From Human Samplesmentioning

confidence: 99%

Outbreak of psittacosis in a group of women exposed to Chlamydia psittaci-infected chickens

Laroucau¹,

Aaziz²,

Meurice

et al. 2015

Eurosurveillance

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Eight cases of psittacosis due to Chlamydia psittaci were identified in May 2013 among 15 individuals involved in chicken gutting activities on a mixed poultry farm in France. All cases were women between 42 and 67 years-old. Cases were diagnosed by serology and PCR of respiratory samples. Appropriate treatment was immediately administered to the eight hospitalised individuals after exposure to birds had been discovered. In the chicken flocks, mainly C. gallinacea was detected, a new member of the family Chlamydiaceae, whereas the ducks were found to harbour predominantly C. psittaci, the classical agent of psittacosis. In addition, C. psittaci was found in the same flock as the chickens that the patients had slaughtered. Both human and C. psittaci-positive avian samples carried the same ompA genotype E/B of C. psittaci, which is widespread among French duck flocks. Repeated grassland rotations between duck and chicken flocks on the farm may explain the presence of C. psittaci in the chickens. Inspection by the veterinary service led to temporary closure of the farm. All birds had to be euthanised on site as no slaughterhouses accepted processing them. Farm buildings and grasslands were cleaned and/or disinfected before the introduction of new poultry birds.

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Detection of Chlamydia trachomatis DNA using MagNA Pure DNA extraction and Cobas Amplicor CT/NG amplification

Cited by 9 publications

References 10 publications

Diarrhoeagenic Escherichia coli are not a significant cause of diarrhoea in hospitalised children in Kuwait

Diarrhoeagenic Escherichia coli are not a significant cause of diarrhoea in hospitalised children in Kuwait

Evaluation of the new COBAS TaqMan CT test v2.0 and impact on the proportion of new variant Chlamydia trachomatis by the introduction of diagnostics detecting new variant C trachomatis in Orebro county, Sweden

Outbreak of psittacosis in a group of women exposed to Chlamydia psittaci-infected chickens

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