Detection of Chlamydia trachomatis DNA in joints of reactive arthritis patients by polymerase chain reaction

Taylor‐Robinson, David; Gilroy, C B; Thomas, B J; Keat, Andrew

doi:10.1016/0140-6736(92)90399-n

Cited by 178 publications

(100 citation statements)

References 7 publications

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“…Several PCR assays targeting chlamydial D N A have been developed and used to examine the question of whether chlamydial nucleic acids are present in the synovia of patients with RS, ReA, o r other arthropathies (14). Some of these analyses have shown chlamydial D N A to be present in RS synovia a t high frequency, while others have indicated that it is not present (1,16).…”

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confidence: 99%

Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction

Branigan

Gérard

Hudson

et al. 1996

Arthritis & Rheumatism

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Objective. Difficulties in detecting Chlamydia trachomatis in human joints by polymerase chain reaction (PCR) may be related to whether synovial tissue or synovial fluid (SF) is used as the source of DNA in PCR amplification. In this study, a new PCR assay was developed and used to compare chlamydial DNA in paired samples of SF and synovial tissue from patients with arthritis.Methods. The PCR assay targeted the ribosomal RNA operons, which are present in 2 copies on the C trachomatis chromosome. DNA from several relevant bacteria and chlamydial serovars was used for testing this screening system. The detection of chlamydial DNA in nucleic acid preparations from matched samples of SF and synovial tissue was compared by PCR assay. Samples were obtained from 55 patients, including patients with reactive arthritis, Reiter's syndrome, and other arthropathies.Results. Testing of the PCR screening system confirmed it to be highly specific and sensitive. Use of this assay to screen DNA from SF and synovial tissue samples showed that 29 (53%) of 55 synovial tissue preparations were positive for chlamydial DNA, but only 16 (29%) of the matched SF samples from these 29 patients were similarly positive. Five (9%) of 55 SF samples, but not their tissue counterparts, were positive for chlamydial DNA by PCR.Conclusion. Detection of chlamydial DNA in the joints of patients by PCR gives positive results more often when synovial tissue rather than SF is the source of target nucleic acids. Although synovial tissue is the source of choice for the most reliable determination of chlamydia in the joint, both synovial tissue and SF should be assayed if possible.

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confidence: 99%

Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction

Branigan

Gérard

Hudson

et al. 1996

Arthritis & Rheumatism

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“…There is growing evidence that Chlamydia can be detected in synovial fluid and synovial membrane not only in acute, but also in chronic, forms of ReA (42). The detection of chlamydia1 DNA (43) and RNA (42,44) confirms that whole viable organisms are likely to be present. However, all attempts thus far to find Yersinia DNA in the joint have failed, in spite of the presence of Yersinia antigen detectable by immunologic techniques (45,46).…”

Section: Bacterial Antigen Persisting In the Joint As The Cause Of Spmentioning

confidence: 95%

Pathogenesis of spondylarthropathies

Sieper

Braun

1995

Arthritis & Rheumatism

152

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“…O diagnóstico da infecção clamidial inclui a cultura, os testes para detecção de antígenos, como a imunofluorescência direta (IFD) e o ensaio imunoenzimático (EIA), a pesquisa de anticorpos por imunofluorescência indireta (IFI), ELISA (enzyme linked immunosorbent assay), os testes de detecção de ácidos nucléicos, como a captura híbrida ou hibridização do DNA, e, mais recentemente, os testes de amplificação do DNA, como a reação em cadeia da polimerase (PCR) e a reação em cadeia da ligase (14) . Este método é considerado como o de escolha na pesquisa do DNA clamidial em materiais sinoviais em decorrência da negatividade da cultura e imunofluorescência na maioria das amostras, e pelo baixo número de células bacterianas presentes nas articulações de pacientes com diferentes artrites (15,16) . A presença da clamídia nas articulações foi demonstrada em 1987, por meio de IFD de células presentes no líquido sinovial (17) .…”

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“…No caso da clamídia, a utilização do exame de amostra de urina do primeiro jato em vez de material urogenital pode servir como uma alternativa não-invasiva para a sua demonstração, por meio de métodos de amplificação molecular como a PCR (15) . Branigan et al (22) levantaram a questão de como a clamídia proveniente do sistema urogenital, o local usual de aquisição da infecção, alcança as articulações.…”

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Detecção do DNA de Chlamydia trachomatis em espondiloartropatias e artrite reumatóide

Fernandez

2005

Rev. Bras. Reumatol.

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Rev Bras Reumatol, v. 45, n. 5, p. 280-90, set./out., 2005 Fernandez et al. 280 ARTIGO ORIGINAL ORIGINAL ARTICLE RESUMOChlamydia trachomatis é a bactéria responsável pela doença sexualmente transmissível mais prevalente no mundo. A maioria das infecções em homens e mulheres é assintomática e, quando não diagnosticada e tratada, pode causar artrite e complicações relacionadas ao aparelho reprodutor feminino.

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Detection of Chlamydia trachomatis DNA in joints of reactive arthritis patients by polymerase chain reaction

Cited by 178 publications

References 7 publications

Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction

Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction

Pathogenesis of spondylarthropathies

Detecção do DNA de Chlamydia trachomatis em espondiloartropatias e artrite reumatóide

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