Detection of Chlamydophila psittaci by Using SYBR Green Real-Time PCR

Okuda, Hideko; Ohya, Kenji; Shiota, Yukihiro; Katô, Hiroshi; Fukushi, Hideto

doi:10.1292/jvms.10-0222

Cited by 9 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The PCR and real-time PCR tests, although highly sensitive and used to detect Chlamydia in different animals in other countries ( 92–96 ) have seldom been used on a large scale due to high cost. The omp1 gene, which is very conservative, was used as a target gene to detect Chlamydia in different animals when IHA results were ambiguous ( 16, 97 ).…”

Section: Diagnostic Methodsmentioning

confidence: 99%

Prevalence, Diagnosis, and Vaccination Situation of Animal Chlamydiosis in China

Zhou

Lou

et al. 2018

Front. Vet. Sci.

View full text Add to dashboard Cite

Since the first case of Chlamydia infection in duck had been reported in 1956 and the first case from domestic animal had been reported in 1979 in China, the chlamydia prevalence in China was heavily according to the published data. The Chlamydia in avian prevalence has been reported at least 11 provinces, Chlamydia in sheep and goats at least 11 provinces, in swine at least 15 provinces, in cows at least 13 provinces and in yaks at least 5 provinces with result of IHA detection. Different diagnostic method such as CFT, ELISA and ABC-ELISA (avidin-biotin-complex ELISA) had been established besides IHA. The inactivated vaccines have been developed with isolated strains from sheep, goats, swine and cows. These inactivated vaccines have been used since 1980s and Chlamydia prevalence in China has been successfully controlled in domestic animal. However, the inactivated vaccines of Chlamydia isolated from avian species have not been successful, although a series of experimental vaccine have been done. Due to the unsustainable eradication plan of Chlamydia in China, sporadic outbreak in animal would happen if the vaccinations were suspended and economy lose in some farmers. Although Chlamydia prevalence in China has a long history, however, almost all published studies are in Chinese, which, in some degree, blocked scientists in other countries to understand the prevalence situation and control measures of Chlamydia in China.

show abstract

Section: Diagnostic Methodsmentioning

confidence: 99%

Prevalence, Diagnosis, and Vaccination Situation of Animal Chlamydiosis in China

Zhou

Lou

et al. 2018

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…DNAs from the specimens were extracted using a PureLink Genomic DNA kit (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's instructions. pecorum with a high sensitivity and a detection limit of 10 inclusion-forming units (IFUs) [19].…”

Section: Specimensmentioning

confidence: 99%

“…The reaction was performed with the Premix Ex-Taq (TaKaRa Bio, Kusatsu, Japan) on a Thermal Cycler Dice TP800 (TaKaRa Bio) in accordance with the manufacturer's instructions. The SYBR Green real-time PCR targeted envB as described previously[19] using the following primers: Env-F (). The reaction was performed with the SYBR Premix Ex-Taq (TaKaRa Bio) on a Thermal Cycler TP800 (TaKaRa Bio) inaccordance with the manufacturer's instructions.…”

mentioning

confidence: 99%

Chlamydial species among wild birds and livestock in the foothills of Mt. Afadjato, Ghana

Sassa-O’Brien

Ohya

Yasuda-Koga

et al. 2022

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

The members of family Chlamydiaceae have a broad host range and cause many kinds of diseases in humans and animals. Several cases of Chlamydiaceae being detected in atypical hosts have been reported recently. Consequently, cross-species monitoring of Chlamydia in wildlife and livestock is pertinent for public health, animal hygiene and wildlife conservation. In this study, we conducted molecular surveillance of Chlamydia in wild birds and livestock around a small village in the foothills of Mt. Afadjato, Ghana where direct contact between wildlife and livestock occurs. Among 29 captured wild birds and 63 livestock, 5 sheep, 30 goats and 28 chickens, the positive ratios of Chlamydia were 24.1%, 40.0%, 43.3% and 26.9%, respectively. Chlamydia pecorum was detected in wild birds, goats, sheep and chickens. On the basis of the variable domain 2 region of ompA, several samples from different hosts showed identical sequences and were phylogenetically located to the same clusters. In addition, using ompA, C. psittaci, C. abortus and C. gallinacea were also detected in this small habitat. Further genetic and pathogenic analyses of the chlamydial distribution in this area, which represents the interface of wild and domestic animal interactions, may improve our knowledge of their transmission among different hosts.

show abstract

“…A curva de dissociação do fragmento de DNA de C. psittaci, amplificado a partir das amostras fecais, apresentou temperatura de dissociação de 83,2 a 83,4ºC (Fig. 1) A positividade para infecção por C. psittaci é variável em função de situações que predispõem as aves à infecção, presença de enfermidade clínica e ao teste diagnóstico utilizado (Stewardson e Grayson, 2010;Okuda et al, 2011). Há relatos desde positividade de 6,3% (2/32) em papagaios-verdadeiros (A. aestiva) e de 26,7% (12/45) em araras-azuis (Anodorhynchus hyacinthinus), procedentes de aves jovens do Pantanal do Mato Grosso do Sul, Brasil (Raso et al, 2006), 52,6% (61/116) em pombos na Espanha (Vázquez et al, 2010) e 90% (43/47) em várias espécies de aves de gaiola na Turquia (Sareyyupoglu et al, 2007).…”

Section: Resultsunclassified

Detecção e classificação molecular de Chlamydophila psittaci em amostras fecais de aves assintomáticas

Braz

Silva

Santiago

et al. 2014

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

RESUMOChlamydophila psittaci é uma bactéria que causa doença respiratória ou sistêmica em aves e em seres humanos. Em vista do risco de transmissão para humanos, o objetivo deste estudo foi detectar a presença de Chlamydophila spp. em amostras de fezes ou suabes cloacais de aves assintomáticas. Foram colhidas 403 amostras fecais ou suabes cloacais, provenientes de aves domésticas, selvagens ou exóticas. As amostras foram submetidas à PCR em tempo real para C. psittaci, para amplificação de fragmento parcial do gene da subunidade 16S do rRNA, utilizando o SsoFast™ EvaGreen® Supermix (Bio-Rad) e análise da curva de dissociação. Para determinação do genótipo de C. psittaci, foi usada a hemi-nested PCR visando à amplificação de fragmento parcial do gene OMP-A, realizada nas amostras positivas pela PCR em tempo real, seguida de sequenciamento dos fragmentos amplificados. A PCR em tempo real revelou positividade em 17 (4,21%) amostras. A hemi-nested foi positiva em 2 amostras positivas pela PCR em tempo real. O genótipo A de C. psittaci foi identificado pelo sequenciamento de uma amostra amplificada pela hemi-nested PCR. Os resultados deste experimento demonstram que a PCR em tempo real, visando à amplificação de fragmento parcial da subunidade 16S do rRNA, seguida da análise da curva de dissociação, pode ser utilizada para detecção de DNA de Chlamydophila sp. em amostras fecais de aves assintomáticas. A classificação da espécie de Chlamydophila e do genótipo de C. psittaci deve ser realizada por meio de PCR tendo como alvo o gene ompA e sequenciamento dos fragmentos amplificados.Palavras-Chave: aves, psitacose, diagnóstico molecular, zoonoses ABSTRACT Chlamydophila psittaci is a bacterium that causes respiratory or systemic disease in birds and humans. Owing to the risk of transmission from asymptomatic birds to humans, the objective of this study was to

show abstract

Detection of Chlamydophila psittaci by Using SYBR Green Real-Time PCR

Cited by 9 publications

References 28 publications

Prevalence, Diagnosis, and Vaccination Situation of Animal Chlamydiosis in China

Prevalence, Diagnosis, and Vaccination Situation of Animal Chlamydiosis in China

Chlamydial species among wild birds and livestock in the foothills of Mt. Afadjato, Ghana

Detecção e classificação molecular de Chlamydophila psittaci em amostras fecais de aves assintomáticas

Contact Info

Product

Resources

About