Detection of chymotrypsin using a peptide sensor based on graphene oxide modified with sulfhydryl groups and gold nanoparticles

Liu, Lei; Liu, Cheng; Bianjiang, Zhang; Gao, Li

doi:10.1039/d2nj02644a

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…As shown in Figure 1a, several characteristic peaks of GO were observed in the FT-IR spectrum including the peaks at 3386, 1718, 1618, and 1050 cm −1 , due to O-H stretching vibration, C=O stretching vibration, C-OH stretching vibration, and C-O stretching vibration, respectively. This showed that GO was successfully synthesized [27]. Figure 1b shows the SEM of GO.…”

Section: Sensitivity For Thrombin Detection Using a Covalent Linking ...mentioning

confidence: 81%

Thrombin Determination Using Graphene Oxide Sensors with Co-Assisted Amplification

Liu

Shi

et al. 2022

Micromachines

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Graphene oxide (GO) is widely used in sensors. The detection of proteins based on bare GO has been developed; however, the detection sensitivity needs to be improved. In this paper, a novel GO-DNA sensor for thrombin detection was developed using an aptamer linked to the surface of GO. Polyethylene glycol (PEG) was further used to prevent thrombin from nonspecific adsorption and to improve the sensitivity of the sensor for detection of thrombin. In order to improve the limit of detection for thrombin, we developed a GO and RecJf exonuclease co-assisted signal amplification strategy, and a detection limit of 24.35 fM for thrombin was achieved using this strategy. The results show that it is a promising method in analytical applications.

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Section: Sensitivity For Thrombin Detection Using a Covalent Linking ...mentioning

confidence: 81%

Thrombin Determination Using Graphene Oxide Sensors with Co-Assisted Amplification

Liu

Shi

et al. 2022

Micromachines

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“…After peptide breakdown by chymotrypsin, AuNPs were more tightly bound to GO, and absorbance was improved. This method achieved a low LOD of 0.25 pg/mL [198].…”

Section: Detection Of Chymotrypsinmentioning

confidence: 91%

Sensing of Digestive Enzymes—Diagnosis and Monitoring of Pancreatitis

Yin,

Cui,

Yang

et al. 2023

Chemosensors

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This paper is a comprehensive review of the techniques for the detection of pancreatic enzymes, which are common biochemical indicators of pancreatitis, including amylase, trypsin, chymotrypsin, elastase, and lipase. Pancreatitis is a disease with self-digestion due to the abnormal activation of digestive enzymes in the pancreas. Hospitalization is often required due to the lack of convenient therapeutic agents. The main recent results are reported in this review, especially the techniques that enable portability and Point-of-Care testing (POCT). This is because timely diagnosis at the early stage and avoiding recurrence after recovery are the keys to treatment. It is also important to reduce the rate of misdiagnosis and to avoid overtreatment. Various detection methods are discussed, with particular attention given to the implementation of chemical sensing and probe design. The new sensing technology for digestive enzymes makes it possible to perform early screening for pancreatitis in remote areas or in one’s own home.

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Detection of chymotrypsin using a peptide sensor based on graphene oxide modified with sulfhydryl groups and gold nanoparticles

Abstract: In this study, GO modified with sulfhydryl group was prepared by thiolation on the surface of GO, which makes a meaningful material. GO with sulfhydryl group combined with gold nanoparticles,...

Cited by 2 publications

References 37 publications

Thrombin Determination Using Graphene Oxide Sensors with Co-Assisted Amplification

Thrombin Determination Using Graphene Oxide Sensors with Co-Assisted Amplification

Sensing of Digestive Enzymes—Diagnosis and Monitoring of Pancreatitis

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