Detection of circulant tumor necrosis factor-alpha , soluble tumor necrosis factor p75 and interferon-gamma in Brazilian patients with dengue fever and dengue hemorrhagic fever

Abstract

Pro-inflammatory cytokines are believed to play an important role in the pathogenesis

Synergistic Effect of TNF-α and Dengue Virus Infection on Adhesion Molecule Reorganization in Human Endothelial Cells

“…Previous in vitro studies have reported significantly increased levels of TNF-a and mRNA levels in DHF/DSS patient serum (16)(17)(18)(19)(20), and it was proposed to have a central role in the development of plasma leakage (1,21). Several studies have reported that TNF-a causes an increase in endothelial permeability and is involved in the disruption and reorganization of the apical junction complex and cytoskeleton proteins (22)(23)(24)(25).…”

“…After 0, 8,16, and 24 h, mock-and TNF-a-treated cells cultured in the absence or presence of DENV-2 were fixed for 10 min using 4z paraformaldehyde in PBS. Cells were washed twice with PBS, blocked for 1 h with 1z bovine serum albumin in PBS, and incubated with anti-PECAM-1 (Santa Cruz Biotechnology) at a dilution of 1:100 at 49 C overnight, and monoclonal antibody against DENV envelope protein (clone 4G2) (20 mg/ml) for 1 h at room temperature.…”

Dengue-2 and yellow fever 17DD viruses infect human dendritic cells, resulting in an induction of activation markers, cytokines and chemokines and secretion of different TNF-α and IFN-α profiles

“…TNF-α is a pro-inflammatory cytokine that has often been found in patients with acute dengue fever and high levels are associated with haemorrhagic manifestations (Hober et al 1993, Kubelka et al 1995, Braga et al 2001, Chakravarti & Kumaria 2006. Together with IL-1β, TNF-α is known to increase acute phase protein production and act in synergy with other factors to induce microbicidal activity during phagocytosis (Clark 2007).…”

“…For dengue fever, tumor necrosis factor (TNF)-α (Hober et al 1993, Braga et al 2001, interleukin (IL)-10 ( Green et al 1999, Azeredo et al 2001, IL-6 (Nguyen et al 2004), macrophage inhibitory factor , Assuncao-Miranda et al 2010 and interferon (IFN)-γ (Bozza et al 2008), among others (Srikiatkhachorn & Green 2010), have been reported. Chemokines also have an important role in pathogenesis.…”

Discovery and Characterization of Potential Prognostic Biomarkers for Dengue Hemorrhagic Fever

“…3 Previously identified markers of dengue severity include cytokines (i.e., tumor necrosis factor alpha [TNFα] and Interleukin 6 [IL-6]), vascular permeability proteins, clotting cascade regulators, and gene expression profiles. [4][5][6][7][8][9][10][11][12][13][14][15][16][17] These severity markers are not, however, currently being used to guide patient management. 18,19 It is unclear why these severity markers are not being used, possibly because detection methods are expensive, slow, or require sophisticated equipment.…”