Detection of circulating antigen in serum of mice infected with Trichinella spiralis by an IgY–IgM mAb sandwich ELISA

Liu, Li Na; Jing, Feng; Cui, Jing; Fu, Guang; Wang, Zhong Quan

doi:10.1016/j.exppara.2012.11.001

Cited by 40 publications

(29 citation statements)

References 34 publications

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“…Additionally, five and eight MAbs generated in this study can recognize the early antigens of the T. spiralis pre-encapsulated larvae in the infected muscle sections at 11 dpi and 13 dpi, respectively. Although the reaction of five MAbs with early antigens of T. spiralis larvae in the sections at 11 dpi was weak, the further sandwich ELISA with mAb 35B9 and 5A7 demonstrated that the detection rate of T. spiralis circulating antigens in serum samples of the infected mice at 11 dpi was only 33.33 % (5/15) and 100 % (20/20), respectively (Wang et al, 2012a;Liu et al, 2013), suggesting that some T. spiralis antigenic epitopes present in infected muscle tissue sections at 11 dpi, which were recognized by mAb 35B9 and 5A7, were also present in early circulating antigens. The results suggested that these MAbs have the potential values for early serodiagnosis of trichinellosis by sandwich ELISA assay to detect the circulating antigens in serum samples of the infected humans and animals.…”

Section: Discussionmentioning

confidence: 99%

Preparation and characterization of monoclonal antibodies against ES antigens of Trichinella spiralis

Cui

Jing

et al. 2014

Helminthologia

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253 SummaryHybridomas secreting monoclonal antibodies (MAbs) against excretory-secretory (ES) antigen of Trichinella spiralis muscle larvae were produced. The 12 monoclones (designated 3A11, 7D9, 7F6, 5A7, 6H7, 39F3, 38H2, 37E7, 35B9, 29A11, 39C9 and 39A6) secreted IgM, IgG3 and IgG2b, respectively. Western blot using T. spiralis ES antigens showed that ten of 12 MAbs recognized the bands between 119.8 -19.3kDa (mainly 68.4 -28.7kDa) and two MAbs (3A11, 37E7) did not recognize any protein constituents of ES antigens. Nine of 12 MAbs also recognized the larval antigens of T. nativa, T. pseudospiralis and T. nelsoni at 40 days post-infection (dpi). No cross-reactions were found with the somatic antigens of adult worms of P. westermani, C. sinensis, and T. solium cysticercus. However, three (3A11, 6H7 and 39A6) were cross-reacted with the somatic antigens of adult worms of S. japonicum. Immunofluorescent location showed that the nine MAbs reacted with the cuticle and internal organs of T. spiralis larvae. Additionally, five and eight MAbs generated in this study can recognize the early antigens of pre-encapsulated T. spiralis larvae at 11 dpi and 13 dpi, respectively. The generation and characterization of the MAbs against T. spiralis ES antigens provide foundation for the development of specific early serodiagnostic techniques for trichinellosis.

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Section: Discussionmentioning

confidence: 99%

Preparation and characterization of monoclonal antibodies against ES antigens of Trichinella spiralis

Cui

Jing

et al. 2014

Helminthologia

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“…All samples were run in duplicate. The ratio <2.1 of samples to be tested/negative control samples (OD values of the samples to be tested divided by OD of the negative controls, S/N < 2.1) were regarded as negative and S/N ≥ 2.1 as positive (Liu et al, 2013). The cut-off value of ELISA was calculated based on the 2.1 times the mean OD value of the negative control serum from healthy individuals.…”

Section: Immunofluorescent Test (Ift)mentioning

confidence: 99%

“…spiralis ML were recovered from the infected mice at 42 dpi by artificial digestion as described previously (Gamble et al, 2000;Li et al, 2010a). The ML crude and ES antigens were prepared as previously described Liu et al, 2013). Adult worms (AW) were isolated from the small intestine of infected mice at 3 and 7 dpi.…”

Section: Collection Of Worms and Preparation Of Crude And Es Antigensmentioning

confidence: 99%

“…However, the main disadvantage of detection of anti-Trichinella antibodies is the occurrence of a high rate of false negative results during the early stage of infection, and the cross-reaction of T. spiralis ES antigens with sera of patients with other parasitic diseases (e.g., paragonimiasis, schistosomiasis, clornorchiasis, cysticercosis, anisakiasis, etc.) (Liu et al, 2013;Yera et al, 2003). Several studies have shown that the maximum detection rate of 100% of IgG antibodies was not reached until at least 1-3 months after human infection with the parasite (Gamble et al, 2004).…”

Section: Introductionmentioning

confidence: 96%

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Characterization of a Trichinella spiralis 31kDa protein and its potential application for the serodiagnosis of trichinellosis

et al. 2015

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“…The mean OD values of positive and negative controls were 0.60 and 0.23, respectively. The ratio <2.1 of samples to be tested/negative sample (OD values of the samples to be tested divided by OD of the negative, S/N < 2.1) were regarded as negative and S/N ≥2.1 as positive (Liu et al, 2013). The cut-off values of ELISA were 0.48.…”

Section: Detection Of Anti-sparganum Antibodies In Serum Samplesmentioning

confidence: 99%

Serological survey for sparganum infection in people of central China

et al. 2014

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SummaryDuring 2006 -2008, twenty cases with sparganosis caused by eating live tadpoles emerged in Henan province, central China. To determine seroprevalence of anti-sparganum antibodies and obtain information about habits of eating live tadpoles and risks for sparganum infection, a serological survey was carried out in one village of Henan. Antisparganum IgG in 298 serum samples were assayed by ELISA using excretory and secretory (ES) antigens of Spirometra mansoni spargana. The results showed 56.71 % (169/298) of inhabitants had the history of eating live tadpoles. The overall seroprevalence was 5.7 % (17/298). The seroprevalence of the inhabitants who had the habit of eating tadpoles (9.47 %) was obviously higher than those who did not (0.78 %) (P < 0.01). Eating live tadpoles had become the most common risk behavior for sparganum infection. Hence, the comprehensive public health education should be carried out in endemic areas, and the habit of eating live tadpoles must be discouraged.

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Detection of circulating antigen in serum of mice infected with Trichinella spiralis by an IgY–IgM mAb sandwich ELISA

Cited by 40 publications

References 34 publications

Preparation and characterization of monoclonal antibodies against ES antigens of Trichinella spiralis

Preparation and characterization of monoclonal antibodies against ES antigens of Trichinella spiralis

Characterization of a Trichinella spiralis 31kDa protein and its potential application for the serodiagnosis of trichinellosis

Serological survey for sparganum infection in people of central China

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