Detection of circulating cancer cells with K-ras oncogene using membrane array

Chen, Y.-F.; Wang, J.-Y.; Wu, Chang‐Yi; Chen, F.-M.; Cheng, Tain-Lu; Lin, Shiu‐Ru

doi:10.1016/j.canlet.2004.12.026

Cited by 58 publications

(71 citation statements)

References 21 publications

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“…Consistent with previous reports, TBX19 was also upregulated in adenoma 21,22) . Because adenoma is considered to be a type of pre -cancerous tumor, we believe that TBX19 plays a role in the ade- 21) .…”

Section: Discussionsupporting

confidence: 93%

“…TBX19 mutations lead to a lack of adrenocorticotrophin resulting in adrenal insufficiency 20) . On the other hand, TBX19 has been identified as one of the genes activated by KRAS mutation, and is upregulated in colon adenoma 21,22) . These results indicate that TBX19 might work as an oncogene in CRC, but the expression and role of TBX19 in CRC remain unknown.…”

Section: Introductionmentioning

confidence: 99%

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<i>TBX19</i> is overexpressed in colorectal cancer and associated with lymph node metastasis

Ando

Saito

Imai

et al. 2017

FJMS

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The T -box 19 (TBX19) gene encodes a transcription factor characterized by a highly conserved DNA -binding motif (T -box). Recent studies have revealed that TBX19 has been identified as one of the genes activated by KRAS mutations, and is upregulated in colon adenoma. These results indicate that TBX19 may work as an oncogene in colorectal cancer (CRC). However, the expression and role of TBX19 have yet to be investigated. Here, we investigated TBX19 mRNA and protein expressions in colon cancer cells or surgically resected CRC. We found that TBX19 mRNA expression was significantly increased in tumorous tissues compared to that in non -tumorous tissues, and increased TBX19 mRNA expression was associated with positive lymph node metastasis in our cohort. The expression of TBX19 mRNA was not correlated with that of TBX19 protein in tissue sample taken from the CRC patients. Moreover, TBX19 showed positive staining even in the normal colonic tissues and the adjacent non -tumorous tissues. These results suggest that the expression of TBX19 protein is not correlated with the expression of TBX19 mRNA. In addition, our results promote further investigations into the impact of TBX19 upregulation on colorectal carcinogenesis, as well as the underlying mechanisms.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

<i>TBX19</i> is overexpressed in colorectal cancer and associated with lymph node metastasis

Ando

Saito

Imai

et al. 2017

FJMS

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show abstract

“…The development of recurrent or metastatic lesions was defined as postoperative relapse. CTCs in the peripheral blood were detected using our previously constructed multigene biomarker chip with serial CEA assays at each follow-up [7,[12][13][14][15]. Additional 4 mL samples of peripheral blood were obtained for total RNA isolation.…”

Section: Follow-upmentioning

confidence: 99%

“…Reports have described the detection of circulating tumor cells (CTCs) in the peripheral blood of CRC patients; this method has major prognostic and therapeutic implications [7][8][9][10]. Our recently developed membrane array-based multigene biomarker assay can detect CTCs in the peripheral blood of CRC patients; this is a rational approach for the surveillance of postoperative CRC patients [6,[11][12][13][14][15]. However, a detailed prospective comparative study regarding the diagnostic accuracy of the biomarker chip and serum CEA level detection is required.…”

Section: Introductionmentioning

confidence: 99%

A prospective study comparing multi-gene biomarker chip and serum carcinoembryonic antigen in the postoperative surveillance for patients with stage I–III colorectal cancer

Wang¹

2016

European Journal of Cancer

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“…24 Visual OMP 3 (Oligonucleotide Modeling Platform, DNA Software, Ann Arbor, MN) was used to design probes for each of the gene targets. The probe selection criteria included strong mismatch discrimination, minimal or no secondary structure, the signal strength at the assay temperature and lack of cross-hybridization.…”

Section: Design Of Oligonucleotide Probes and Preparation Of Oligonucmentioning

confidence: 99%

Development of a high‐throughput membrane‐array method for molecular diagnosis of circulating tumor cells in patients with gastric cancers

Lin

et al. 2006

Intl Journal of Cancer

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Recently several noninvasive methods have been employed to detect circulating tumor cells (CTCs) in cancer patients. In this study, we have developed a highly sensitive, high-throughput colorimetric membrane-array method that was designed to detect a panel of mRNA markers including human telomerase reverse transcriptase (hTERT), , carcinoembryonic antigen (CEA) and mucin 1 (MUC1) mRNA for the presence of CTCs in the peripheral blood of patients with gastric cancer (GC). Digoxigenin-labeled cDNA targets synthesized following total RNA isolation from peripheral blood samples of 64 GC patients and 80 healthy individuals were subjected to membrane-array hybridization. The results showed that membrane array could positively detect 5 cancer cells/ml of peripheral blood in GC cell-dilution experiments. The sensitivity, specificity and diagnostic accuracy for hTERT, CK-19, CEA and MUC1 mRNA ranged from 78.1% to 82.8%, 76.3% to 85% and 81.3% to 83.3%, respectively. Both CEA and MUC1 mRNA expression was correlated significantly with all malignant biological properties of GC, such as macroscopic type, depth of tumor invasion, lymph-node metastasis, TNM stage and coexisting distant metastasis (all p < 0.05). Using these 4 markers in combination, the sensitivity, specificity and diagnostic accuracy of membrane array were raised to 89.1%, 91.3% and 90.3%, respectively. The expression of all 4 mRNA markers was an independent predictor for postoperative recurrence/metastasis. GC patients with the expression of all the 4 mRNA markers showed a poorer survival rate than those without the expression of any 1 mRNA marker (p 5 0.0223). These findings demonstrated that our membrane-array method could detect CTCs in the circulation of GC patients with considerably high sensitivity and specificity. The identification of CTCs in the peripheral blood may be useful in the auxiliary cancer diagnostics or postoperative surveillance of GC patients for recurrence/metastasis. ' 2006 Wiley-Liss, Inc.Key words: circulating tumor cell; membrane array; gastric cancer; molecular diagnosisThe degrees of tumor penetration in the stomach wall and lymph node metastasis have been used for many decades as the 2 major prognostic determinants for gastric cancer (GC) patients. Unfortunately, despite informative staging of patients with GC, some patients with apparently localized disease at diagnosis will subsequently develop recurrent or metastatic diseases. 1-4 One of the major causes is attributed to the presence of disseminated tumor cells shed from the primary carcinoma into circulation, prior to or during surgery. Even in patients with early GC, blood-born metastasis occurs. 2,5,6 Recent attempts to improve staging include sensitive detection of disseminated tumor cells in the blood or lymph nodes by molecular approaches. Evidence increasingly clarifies that primary cancers begin shedding neoplastic cells into the circulation at an early stage. [7][8][9] It is the dissemination of cancer cells into circulation that is widely accepted as the main cause l...

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Detection of circulating cancer cells with K-ras oncogene using membrane array

Cited by 58 publications

References 21 publications

<i>TBX19</i> is overexpressed in colorectal cancer and associated with lymph node metastasis

<i>TBX19</i> is overexpressed in colorectal cancer and associated with lymph node metastasis

A prospective study comparing multi-gene biomarker chip and serum carcinoembryonic antigen in the postoperative surveillance for patients with stage I–III colorectal cancer

Development of a high‐throughput membrane‐array method for molecular diagnosis of circulating tumor cells in patients with gastric cancers

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