Detection of circulating immunocomplexes in patients diagnosed with acute brucellosis

Plata, J.; Leiva, José; Martinez, ML; Mendoza, Joaquín; Navarro, JM; Fraile, Manuel de la Rosa

doi:10.1016/0888-0786(94)90019-1

Cited by 1 publication

(1 citation statement)

References 15 publications

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“…For determination of immune complex-associated antibodies, immune complexes were precipitated from 100 µl serum by 3n5 % polyethyleneglycol 6000. The complexes were dissociated overnight in Tris-HCl buffer, pH 10n2 at 4 mC, and immediately tested, diluted 1 : 10, in the enzyme immunoassays [16]. Peroxidase-labelled rabbit antihuman IgG ad IgA (Dako, ITK Diagnostics, Uithoorn, The Netherlands) was used to detect bound antibodies.…”

Section: Serological Methodsmentioning

confidence: 99%

Chlamydia pneumoniae is a risk factor for coronary heart disease in symptom-free elderly men, but Helicobacter pylori and cytomegalovirus are not

et al. 1998

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To test the hypothesis that chronic infection with Chlamydia pneumoniae, Helicobacter pylori or cytomegalovirus is associated with coronary heart disease risk in elderly men, a nested case-control study in a cohort investigated in 1985 and 1990 in the town of Zutphen, The Netherlands, was designed. Fifty-four cases with a first diagnosed coronary event between 1985 and 1990, and 108 age-matched control subjects free of coronary heart disease during follow up were included in the study. The overall prevalence of antibodies to cytomegalovirus was 74.7%, to H. pylori 75.9% and to C. pneumoniae 84.0%. A high level of antibodies to C. pneumoniae was associated with an increased coronary heart disease risk (OR = 2.76; 95% CI = 1.31-5.81). This association was stronger in cases developing both myocardial infarction and angina pectoris, than in cases developing only one of these. This association was independent of potential confounders. Antibodies to cytomegalovirus or H. pylori were not associated with coronary heart disease risk. These results support the hypothesis of a role of chronic C. pneumoniae infections in the immunopathogenesis of atherosclerosis.

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Section: Serological Methodsmentioning

confidence: 99%