Detection of Circulating Stromal Stem Cells with Osteogenic Potential in the Blood of Coronary Patients by Laser Flow Cytometry

Gabbasov, Zufar; Agapov, A.A.; Saburova, O.; Obedzinskii, E. A.; Soboleva, E.L.

doi:10.1007/s10517-005-0266-6

Cited by 3 publications

(6 citation statements)

References 7 publications

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“…While novel, our findings are nonetheless consistent with recent reports by Gabbasov et al comparing circulating cells staining with an antibody for another bone-related protein, osteonectin, in patients with CAD compared with normal subjects (36,37). In those studies, osteonectin-positive cells were not characterized as EPCs; however, the authors did find that osteonectin-positive cells were increased in the circulation of CAD as compared to control patients.…”

Section: Discussionsupporting

confidence: 92%

Osteocalcin Expression by Circulating Endothelial Progenitor Cells in Patients With Coronary Atherosclerosis

Gössl

Mödder

Atkinson

et al. 2008

Journal of the American College of Cardiology

155

148

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OBJECTIVES To test whether patients with coronary atherosclerosis have increases in circulating endothelial progenitor cells (EPCs) expressing an osteogenic phenotype. BACKGROUND Increasing evidence indicates a link between bone and the vasculature, and bone marrow and circulating osteogenic cells have been identified by staining for the osteoblastic marker, osteocalcin (OCN). EPCs contribute to vascular repair, but repair of vascular injury may result in calcification. Using cell surface markers (CD34, CD133, KDR) to identify EPCs, we examined whether patients with coronary atherosclerosis had increases in the percentage of EPCs expressing OCN. METHODS We studied 72 patients undergoing invasive coronary assessment: Controls (normal coronary arteries and no endothelial dysfunction, n = 21) versus two groups with coronary atherosclerosis: early coronary atherosclerosis (ECA: normal coronary arteries but with endothelial dysfunction, n = 22) and late coronary atherosclerosis (LCA: severe, multi-vessel coronary artery disease [CAD], n = 29). Peripheral blood mononuclear cells were analyzed using flow cytometry. RESULTS Compared to controls, patients with ECA or LCA had significant increases (~2-fold) in the percentage of CD34+/KDR+ and CD34+/CD133+/KDR+ cells co-staining for OCN; even larger increases were noted in the ECA and LCA patients in the percentage of CD34+/CD133−/KDR+ cells co-staining for OCN (5- and 2-fold, P < 0.001 and 0.05, respectively). CONCLUSIONS A higher percentage of EPCs express OCN in patients with coronary atherosclerosis compared with subjects with normal endothelial function and no structural CAD. These findings have potential implications for the mechanisms of vascular calcification and for the development of novel markers for coronary atherosclerosis.

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Section: Discussionsupporting

confidence: 92%

Osteocalcin Expression by Circulating Endothelial Progenitor Cells in Patients With Coronary Atherosclerosis

Gössl

Mödder

Atkinson

et al. 2008

Journal of the American College of Cardiology

155

148

View full text Add to dashboard Cite

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“…The results of proteomic studies indicated high level of stromal stem cell marker osteonectin in the blood of CA patients, which was in line with published data [1,[6][7][8][9]. High level of osteonectin-positive cells was suggested as an indicator of the presence and progress of atherosclerotic involvement of the vessels in humans [1].…”

Section: Resultssupporting

confidence: 87%

“…High level of osteonectin-positive cells was suggested as an indicator of the presence and progress of atherosclerotic involvement of the vessels in humans [1]. Low level of CD34 stem cells in patients with CHD and CA in comparison with healthy individuals can be due to intensive infuzation of these cells in zones of endothelial destruction and atherosclerotic foci [9].…”

Section: Resultsmentioning

confidence: 99%

“…Presumably, cells proliferating in the vascular intima are of bone marrow origin, and bone marrow colony-forming stem cells of hemopoietic and stromal differentiation lineages penetrate into the vascular intima in sites of lipid concentration and development of atherosclerotic foci [1,6,12]. CD34, highly glycosylated type 1 transmembrane pro tein (sialomucin) is a marker of hemopoietic stem cells, while stromal stem cells carry on their surface osteonectin, a non-collagen bone tissue glycoprotein selectively binding calcium and phosphorus salts to collagen.…”

mentioning

confidence: 99%

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Blood Level of Osteonectin in Stenosing Atherosclerosis and Calcinosis of Coronary Arteries

Ragino¹,

Kashtanova

Chernjavski

et al. 2011

Bull Exp Biol Med

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Blood levels of stem cell marker proteins CD34 and osteonectin were studied in male patients with coronary atherosclerosis by direct biomagnetic separation of proteins with magnetic microspheres using the PureProteome Protein A and Protein G Magnetic Beads proteomic technology. High concentration of osteonectin in the blood was detected, particularly in men with stenosing atherosclerosis and coronary artery calcinosis. Blood osteonectin concentration correlated significantly with some key biomarkers of atherosclerosis and with stenosing atherosclerosis and calcinosis of coronary arteries. The results indicate that osteonectin as a marker of stromal stem cells with osteogenic potential presumably plays an important role in atherogenesis and can serve as a new biomarker of stenosing atherosclerosis and calcinosis of coronary arteries.

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“…38 Another potential role for the EPC from mesenchymal origin may be in determining the kind of vascular repair such as coronary calcification, with EPC that carry an osteogenic potential. 39 The extension of endothelial dysfunction may be seen in two distinct ways. On the one hand it may extend into bone marrow microvascular endothelial dysfunction (i.e., the release of EPC from the bone marrow is impaired as a result of loss of efferent vessels).…”

Section: Microcellular Endothelial Dysfunction Of Epcmentioning

confidence: 99%

Frontiers in Nephrology

Gössl

Lerman

2007

Journal of the American Society of Nephrology

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Detection of Circulating Stromal Stem Cells with Osteogenic Potential in the Blood of Coronary Patients by Laser Flow Cytometry

Cited by 3 publications

References 7 publications

Osteocalcin Expression by Circulating Endothelial Progenitor Cells in Patients With Coronary Atherosclerosis

Osteocalcin Expression by Circulating Endothelial Progenitor Cells in Patients With Coronary Atherosclerosis

Blood Level of Osteonectin in Stenosing Atherosclerosis and Calcinosis of Coronary Arteries

Frontiers in Nephrology

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