Detection of Circulating Trophoblast Particles in Peripheral Maternal Blood in Preeclampsia Complicated Pregnancies

Hoffmann, Barbara; Beelen, Dietrich W.; Gellhaus, Alexandra; Winterhager, Elke; Kasimir‐Bauer, Sabine

doi:10.1080/10641950701885170

Cited by 18 publications

(19 citation statements)

References 26 publications

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“…The mean length of SNAs was 72 ± 21 μm (range 42-116), which is comparable to the size of SNAs reported in various vascular beds in vivo (decidual vein 50 μm [16] , broad ligament vein 40-75 μm [17] , uterine vein 10-200 μm [20,24,35] , lungs 12-150 μm [22,[36][37][38] and peripheral circula- [16,17,[24][25][26][27][28] ). In addition, we observed 3 general morphologies/shapes for our SNAs; teardrop, rounded and irregular, which are also the same as those reported for SNAs in vivo [24] .…”

Section: Dna Content and Metabolic Activity Of Snassupporting

confidence: 71%

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Detection of Fetal Sex, Aneuploidy and a Microdeletion from Single Placental Syncytial Nuclear Aggregates

Holland

Kroneis²,

El‐Heliebi³

et al. 2016

Fetal Diagn Ther

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Objectives: A key problem in prenatal screening using extra-embryonic cells is the feasibility of extracting usable DNA from a small number of cells. Syncytial nuclear aggregates (SNAs) are multinucleated structures shed from the placenta. This study assesses the potential of SNAs as a source of fetal DNA for the detection of genetic abnormalities. Methods: SNAs were collected in vitro. Whole-genome amplification was used to amplify DNA from single SNAs, and DNA quality and quantity was assessed by spectrophotometry and PCR. Confocal microscopy was used to count nuclei within SNAs, determine metabolic activity and investigate DNA damage. Fetal sex and chromosomal/genetic abnormalities were investigated with array-comparative genomic hybridization (aCGH). Results: DNA was amplified from 81% of the individual SNAs. A mean of 61 ± 43 nuclei were found per SNA. DNA strand breaks were found in 76% of the SNAs. Seventy-five percent of SNAs yielded whole-genome-amplified DNA of sufficient quality for aCGH after storage and shipping. Individual SNAs from the same pregnancy reliably gave the same chromosomal profile, and fetal sex and trisomies could be detected. A microdeletion was detected in one pregnancy. Conclusion: SNAs could provide a source of extra-embryonic DNA for the prenatal screening/diagnosis of fetal sex and chromosomal and sub-chromosomal genetic abnormalities.

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Section: Dna Content and Metabolic Activity Of Snassupporting

confidence: 71%

“…Despite this challenge, several groups have reported successful isolation of SNAs from maternal peripheral blood [16,17,[24][25][26][27][28] as early as at 6 weeks of gestation [25] . Since SNAs may contain predominantly fragmented DNA, the quality and utility of the DNA may not be adequate for use in diagnostics, but this has not been tested.…”

mentioning

confidence: 99%

Detection of Fetal Sex, Aneuploidy and a Microdeletion from Single Placental Syncytial Nuclear Aggregates

Holland

Kroneis²,

El‐Heliebi³

et al. 2016

Fetal Diagn Ther

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“…They showed that after the 30th gestational week, OPN is only present in cytotrophoblasts of preeclamptic placentas, but not in healthy ones (53). It was previously reported that a significantly higher amount of trophoblast debris can be found in the maternal circulation in preeclampsia compared to women with a normal pregnancy (41,42,54). Based on these observations, we examined the association between the concentrations of circulating fetal DNA as a marker of trophoblast debris, and OPN in maternal blood.…”

Section: Discussionmentioning

confidence: 96%

Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?

Stenczer

Rigó

Prohászka

et al. 2009

Clinical Chemistry and Laboratory Medicine

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Background: It has been previously reported that plasma osteopontin (OPN) concentrations are increased in cardiovascular disorders. The goal of the present study was to determine plasma OPN concentrations in healthy pregnant women and preeclamptic patients, and to investigate their relationship to the clinical characteristics of the study subjects and to markers of inflammation wC-reactive protein (CRP)x, endothelial activation wvon Willebrand factor antigen (VWF:Ag)x or endothelial injury (fibronectin), oxidative stress wmalondialdehyde (MDA)x and trophoblast debris (cellfree fetal DNA). Methods: Forty-four patients with preeclampsia and 44 healthy pregnant women matched for age and gestational age were involved in this case-control study. Plasma OPN concentrations were measured with ELISA. Serum CRP concentrations were determined with an autoanalyzer using the manufacturer's reagents. Plasma VWF:Ag was quantified by ELISA, while plasma fibronectin concentrations were measured by nephelometry. Plasma MDA concentrations were estimated by the thiobarbituric acid-based colorimetric assay. The amount of cell-free fetal DNA in maternal plasma was determined by quantitative real-time PCR analysis of the sexdetermining region Y (SRY) gene. For statistical analyses, non-parametric methods were applied.

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“…We recently demonstrated that the number of trophoblast cells in maternal blood of preeclamptic pregnancies was significantly higher than in non-preeclamptic pregnancies. Whereas the fetal origin of the detected cells were verified by XY-FISH analysis in women delivering a boy, for women delivering a girl, no additional characterization was possible [18] .…”

Section: Discussionmentioning

confidence: 98%

“…The fetal origins of the detected cells were verified by XY-FISH analysis in women delivering a boy. Unfortunately, for women delivering a girl, no additional characterization was possible [18] .…”

Section: Introductionmentioning

confidence: 99%

mRNA of Placental Origin in Maternal Serum of Women with Normal and Preeclamptic Pregnancies

Hoffmann¹,

Kasimir‐Bauer²

2009

Fetal Diagn Ther

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Objective: We quantified placenta-specific mRNA from maternal blood to evaluate differences in normal and preeclamptic pregnancies. Methods: mRNA was purified from 560 μl serum using the QIAamp® Viral RNA Mini Kit in 8 women with normal and 8 women with preeclamptic pregnancies. Results: The relative amounts for β-hCG, CRH and hPL in serum samples of each study group showed a broad variety in the expression levels within one group. Higher amounts for CRH were found in most cases of preeclampsia, for β-hCG and hPL the amounts for women with preeclamptic pregnancies were tenfold below the values found for normal pregnancies. Conclusion: This method is not useful to distinguish between normal and preeclamptic pregnancies in the second trimester and later. Whether it can be applied for early diagnosis of preeclampsia has to be elucidated in further studies.

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Detection of Circulating Trophoblast Particles in Peripheral Maternal Blood in Preeclampsia Complicated Pregnancies

Abstract: The number of trophoblast particles in peripheral maternal blood of preeclamptic pregnancies was significantly higher than in non-preeclamptic pregnancies.

Cited by 18 publications

References 26 publications

Detection of Fetal Sex, Aneuploidy and a Microdeletion from Single Placental Syncytial Nuclear Aggregates

Detection of Fetal Sex, Aneuploidy and a Microdeletion from Single Placental Syncytial Nuclear Aggregates

Plasma osteopontin concentrations in preeclampsia – is there an association with endothelial injury?

mRNA of Placental Origin in Maternal Serum of Women with Normal and Preeclamptic Pregnancies

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