Detection of clinically relevant antimicrobial resistance determinants in warm-blooded marine animals in Livingston Island (South Shetland Islands, Antarctica): A field-based molecular genetics study

Dimov, Svetoslav

doi:10.1016/j.marpolbul.2022.113751

Cited by 3 publications

(5 citation statements)

References 23 publications

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“…Despite the fact that we do not have any proof that this is also the case in this research, such a hypothesis could not be completely excluded. A basis for such speculation is the fact that microplastics have also been implicated as a potential vector for the spread of antibiotic-resistant bacteria [44,45], as well as some published findings during the same Antarctic expedition reporting the presence of antibiotic-resistance genes in animals around the Bulgarian Antarctic base [12]. Surprisingly, sample S10 contained a very high abundance of a single genus-Tetracladium, which accounted for half of all reads and has been shown to have biotechnological potential for producing pectinolytic enzymes [46].…”

Section: Community Composition Of Microbial Surface Mats On Submerged...mentioning

confidence: 99%

“…Additionally, Antarctica's pristine and isolated environments provide an excellent opportunity to study the effects of anthropogenic factors on microbial communities [10]. Research stations in Antarctica are recognized sources of anthropogenic influence on the continent, attributed to factors such as the deposition of microplastics from equipment [11] and an increase in antimicrobial-resistant bacteria [12,13].…”

Section: Introductionmentioning

confidence: 99%

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Study of the Bacterial, Fungal, and Archaeal Communities Structures near the Bulgarian Antarctic Research Base “St. Kliment Ohridski” on Livingston Island, Antarctica

Doytchinov,

Peykov,

Dimov

2024

Life

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As belonging to one of the most isolated continents on our planet, the microbial composition of different environments in Antarctica could hold a plethora of undiscovered species with the potential for biotechnological applications. This manuscript delineates our discoveries after an expedition to the Bulgarian Antarctic Base “St. Kliment Ohridski” situated on Livingston Island, Antarctica. Amplicon-based metagenomics targeting the 16S rRNA genes and ITS2 region were employed to assess the metagenomes of the bacterial, fungal, and archaeal communities across diverse sites within and proximal to the research station. The predominant bacterial assemblages identified included Oxyphotobacteria, Bacteroidia, Gammaprotobacteria, and Alphaprotobacteria. A substantial proportion of cyanobacteria reads were attributed to a singular uncultured taxon within the family Leptolyngbyaceae. The bacterial profile of a lagoon near the base exhibited indications of penguin activity, characterized by a higher abundance of Clostridia, similar to lithotelm samples from Hannah Pt. Although most fungal reads in the samples could not be identified at the species level, noteworthy genera, namely Betamyces and Tetracladium, were identified. Archaeal abundance was negligible, with prevalent groups including Woesearchaeales, Nitrosarchaeum, Candidatus Nitrosopumilus, and Marine Group II.

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Section: Community Composition Of Microbial Surface Mats On Submerged...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Study of the Bacterial, Fungal, and Archaeal Communities Structures near the Bulgarian Antarctic Research Base “St. Kliment Ohridski” on Livingston Island, Antarctica

Doytchinov,

Peykov,

Dimov

2024

Life

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show abstract

“…Additionally, Antarcticaʹs pristine and isolated environments provide an excellent opportunity to study the effects of anthropogenic factors on microbial communities [10]. Research stations in Antarctica are recognized sources of anthropogenic influence on the continent, attributed to factors such as the deposition of microplastics from equipment [11] and an increase in antimicrobial-resistant bacteria [12,13].…”

Section: Introductionmentioning

confidence: 99%

Study of the Bacterial, Fungal, and Archaeal Communities Structures near the Bulgarian Antarctic Research Base “St. Kliment Ohridski” on Livingston Island, Antarctica

Doytchinov,

Peykov,

Dimov

2024

Preprint

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As belonging to one of the most isolated continents on our planet, the microbial composition of different environments in Antarctica could hold a plethora of undiscovered species with potential for biotechnological applications. This manuscript delineates our discoveries after an expedition to the Bulgarian Antarctic Base "St. Kliment Ohridski" situated on Livingston Island, Antarctica. Amplicon-based metagenomics targeting the 16S rRNA genes and ITS2 region were employed to assess the metagenomes of the bacterial, fungal and archaeal communities across diverse sites within and proximal to the research station. The predominant bacterial assemblages identified included Oxyphotobacteria, Bacteroidia, Gammaprotobacteria and Alphaprotobacteria. A substantial proportion of cyanobacteria reads were attributed to a singular uncultured taxon within the family Leptolyngbyaceae. The bacterial profile of a lagoon near the base exhibited indications of penguin activity, characterized by a higher abundance of Clostridia, similar to lithotelm samples from Hannah Pt. Although most fungal reads in the samples could not be identified at the species level, noteworthy genera, namely Betamyces and Tetracladium, were identified. Archaeal abundance was negligible, with prevalent groups including Woesearchaeales, Nitrosarchaeum, Candidatus Nitrosopumilus and Marine Group II.

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“…Therefore, penguins may act as vectors of ARGs and promote their dissemination into Antarctic environments through their nesting and guano deposition. 36,37 In coastal Antarctica, a large amount of penguin guano can be ultimately transported into lakes through ice-snow melting water. The deposition of penguin guano strongly influences the biogeochemical properties of lake sediments around their colonies and produces a kind of special sediments, named ornithogenic lake sediments (OLS), which are particularly rich in organic matter, nitrogen, and phosphorus.…”

Section: ■ Introductionmentioning

confidence: 99%

“…PCR screening for sulphonamide resistance produced positive results in penguin guano samples. , Furthermore, penguins may have carried the resistant pathogen from elsewhere following migration, and they excrete large amounts of guano into the terrestrial ecosystem in Antarctica during nesting and breeding. Therefore, penguins may act as vectors of ARGs and promote their dissemination into Antarctic environments through their nesting and guano deposition. , …”

Section: Introductionmentioning

confidence: 99%

Penguin-Driven Dissemination and High Enrichment of Antibiotic Resistance Genes in Lake Sediments across Antarctica

Zhou,

Zhu,

Song

et al. 2024

Environ. Sci. Technol.

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Numerous penguins can propagate pathogens with antibiotic resistance genes (ARGs) into Antarctica. However, the effects of penguin dissemination on the lake ARGs still have received little attention via guano deposition. Here, we have profiled ARGs in ornithogenic sediments subject to penguin guano (OLS) and nonornithogenic sediments (NOLS) from 16 lakes across Antarctica. A total of 191 ARGs were detected in all sediment samples, with a much higher abundance and diversity in OLS than in NOLS. Surprisingly, highly diverse and abundant ARGs were found in the OLS with a detection frequency of >40% and an absolute abundance of (2.34 × 10 9 )−(4.98 × 10 9 ) copies g −1 , comparable to those in coastal estuarine sediments and pig farms. The strong correlations of identified resistance genes with penguin guano input amount, environmental factors, mobile genetic elements, and bacterial community, in conjunction with network and redundancy analyses, all indicated that penguins were responsible for the dissemination and high enrichment of ARGs in lake sediments via the guano deposition, which might greatly outweigh local human-activity effects. Our results revealed that ARGs could be carried into lakes across the Antarctica through penguin migration, food chains, and guano deposition, which were closely connected with the widespread pollution of ARGs at the global scale.

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Detection of clinically relevant antimicrobial resistance determinants in warm-blooded marine animals in Livingston Island (South Shetland Islands, Antarctica): A field-based molecular genetics study

Cited by 3 publications

References 23 publications

Study of the Bacterial, Fungal, and Archaeal Communities Structures near the Bulgarian Antarctic Research Base “St. Kliment Ohridski” on Livingston Island, Antarctica

Study of the Bacterial, Fungal, and Archaeal Communities Structures near the Bulgarian Antarctic Research Base “St. Kliment Ohridski” on Livingston Island, Antarctica

Study of the Bacterial, Fungal, and Archaeal Communities Structures near the Bulgarian Antarctic Research Base “St. Kliment Ohridski” on Livingston Island, Antarctica

Penguin-Driven Dissemination and High Enrichment of Antibiotic Resistance Genes in Lake Sediments across Antarctica

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