Detection of clonal T-cell receptor γ chain gene rearrangements by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE) in archival specimens from patients with early cutaneous T-cell lymphoma: Correlation of histologic findings with PCR/DGGE

Tok, Julide; Szabolcs, Matthias; Silvers, David N.; Zhong, Jingyao; Matsushima, Anne Y.

doi:10.1016/s0190-9622(98)70505-5

Cited by 47 publications

(45 citation statements)

References 18 publications

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“…2,3,[5][6][7]16,21,35,50 The incidence of false-negative results and the increasingly frequent identification of monoclonal populations of T lymphocytes in many reactive disorders suggest that results of molecular analyses must be evaluated in the context of clinicopathologic correlation. 2,3,[5][6][7]16,21,35,50 The incidence of false-negative results and the increasingly frequent identification of monoclonal populations of T lymphocytes in many reactive disorders suggest that results of molecular analyses must be evaluated in the context of clinicopathologic correlation.…”

Section: Discussionmentioning

confidence: 99%

Histopathologic Features of Early (Patch) Lesions of Mycosis Fungoides

Massone¹,

Kodama²,

Kerl³

et al. 2005

American Journal of Surgical Pathology

178

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The histologic diagnosis of early mycosis fungoides (MF) is one of the most vexing problems in dermatopathology. We reviewed the histopathologic features of 745 biopsy specimens from 427 patients (male:female = 277:150; median age, 52 years; range, 3-95 years) with early (patch) lesions of MF collected from the lymphoma database of the Department of Dermatology of the Medical University of Graz (Austria). In all patients, the diagnosis was established by clinicopathologic correlation. The most common histopathologic pattern consisted of a band-like or patchy lichenoid infiltrate admixed with coarse bundles of collagen in the superficial dermis. Epidermotropism of lymphocytes was observed in most cases in one or more forms (single lymphocyte epidermotropism, 22%; basilar lymphocytes, 23%; Pautrier's microabscesses, 19%; "haloed" lymphocytes, 40%; disproportionate exocytosis, 17%; pagetoid epidermotropism, 3%). In 4% of cases, epidermotropism was completely missing. Atypical lymphocytes were present only in 9% of cases. Features of interface dermatitis were observed in 59% of cases. Other unusual findings were the presence of necrotic keratinocytes (23%), melanophages (8%), and extravasated erythrocytes (4%). In 28 patients, two or more biopsies taken on the same day at different body sites showed different histopathologic aspects, underlying the protean features of MF even in a single patient at a given time. Our study expands previous observations on histopathologic features of early lesions of MF. Although sometimes the histopathologic features are not diagnostic, they should be considered consistent with MF and do not rule out the diagnosis.

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Section: Discussionmentioning

confidence: 99%

Histopathologic Features of Early (Patch) Lesions of Mycosis Fungoides

Massone¹,

Kodama²,

Kerl³

et al. 2005

American Journal of Surgical Pathology

178

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“…DNA cytophotometry, 33-37 nuclear morphometry, 38-41 immunohistochemistry, 42-57 chromosomal studies, [58][59][60] and, more recently, molecular genetic analysis of T-cell clonality 8,[55][56][57][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78] have generated a significant amount of data that suggest their utility not only in the early diagnosis of MF but also potentially in the clinical management of MF patients. How these data may be integrated with light microscopy to aid in the definitive diagnosis of CTCL has not been standardized.…”

Section: Early Diagnosis Of Mf: the Role Of Ancillary Techniquesmentioning

confidence: 99%

Defining early mycosis fungoides

Pimpinelli¹,

Olsen

Santucci³

et al. 2005

Journal of the American Academy of Dermatology

476

378

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This editorial review summarizes the results of 5 meetings sponsored by the International Society for Cutaneous Lymphoma at which the clinicopathologic and ancillary features of early mycosis fungoides were critically examined. Based on this analysis, an algorithm was developed for the diagnosis of early mycosis fungoides involving a holistic integration of clinical, histopathologic, immunopathologic, and molecular biological characteristics. A novel aspect of this algorithm is that it relies on multiple types of criteria rather than just one, for example, histopathology. Before its finalization, the proposed diagnostic algorithm will require validation and possibly further refinement at multiple centers during the next several years. It is anticipated that a more standardized approach to the diagnosis of early mycosis fungoides will have a beneficial impact on the epidemiology, prognostication, treatment, and analysis of clinical trials pertaining to this most common type of cutaneous lymphoma.

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“…One such technique, clonal rearrangements of the T-cell receptor (TCR) gamma gene by polymerase chain reaction (PCR) are positive only in 74% of early MF biopsies. [7] Further, sensitivity of a given marker has been shown to vary with technique used and the density of tumor cells in a sample. [8] Early-stage MF is generally indolent with little effect on overall life expectancy; however, rapid progression and extracutaneous spread of malignant T cells occur in a subset of patients.…”

Section: Introductionmentioning

confidence: 99%

The biomarker landscape in mycosis fungoides and Sézary syndrome

Dulmage

Geskin

Guitart

et al. 2017

Experimental Dermatology

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The practice of pre-emptive individualized medicine is predicated on the discovery, development and application of biomarkers in specific clinical settings. Mycosis fungoides and Sézary syndrome are the two most common type of cutaneous T-cell lymphoma, yet diagnosis, prognosis and disease monitoring remain a challenge. In this review, we discuss the current state of biomarker discovery in mycosis fungoides and Sézary syndrome, highlighting the most promising molecules in different compartments. Further, we emphasize the need for continued multicentre efforts to validate available and new biomarkers and to develop prospective combinatorial panels of already discovered molecules.

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Detection of clonal T-cell receptor γ chain gene rearrangements by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE) in archival specimens from patients with early cutaneous T-cell lymphoma: Correlation of histologic findings with PCR/DGGE

Cited by 47 publications

References 18 publications

Histopathologic Features of Early (Patch) Lesions of Mycosis Fungoides

Histopathologic Features of Early (Patch) Lesions of Mycosis Fungoides

Defining early mycosis fungoides

The biomarker landscape in mycosis fungoides and Sézary syndrome

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