Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin

Ganesh, Madhu; Sheikh, Nasira K.; Shah, Pooja; Mehetre, Gajanan T.; Dharne, Mahesh; Nagoba, Basavraj

doi:10.1016/j.jiph.2015.06.014

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…Bacterial DNA is commonly extracted from enrichment broth cultures or colonies on agar plates with commercial DNA extraction kits. 2,25,[41][42][43] In conventional PCR, the readout is performed by electrophoresis of DNA on an agar gel stained with ethidium bromide. More accurately, the sequencing of PCR products gives unambiguous results.…”

Section: Identification Of Tent-containing Clostridiamentioning

confidence: 99%

Tetanus in animals

Popoff

2020

J VET Diagn Invest

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Tetanus is a neurologic disease of humans and animals characterized by spastic paralysis. Tetanus is caused by tetanus toxin (TeNT) produced by Clostridium tetani, an environmental soilborne, gram-positive, sporulating bacterium. The disease most often results from wound contamination by soil containing C. tetani spores. Horses, sheep, and humans are highly sensitive to TeNT, whereas cattle, dogs, and cats are more resistant. The diagnosis of tetanus is mainly based on the characteristic clinical signs. Identification of C. tetani at the wound site is often difficult.

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Section: Identification Of Tent-containing Clostridiamentioning

confidence: 99%

Tetanus in animals

Popoff

2020

J VET Diagn Invest

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“…However, C. tetani are difficult to isolate due to the characteristics of anaerobic bacteria (12,13). Recently, the detection of tetanospasmin in C. tetani using real-time polymerase chain reaction (PCR) has become possible, and it has been used to diagnose tetanus in livestock (14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Successful treatment of idiopathic tetanus using metronidazole, magnesium, and acepromazine in Hanwoo (Korean indigenous cattle) yearling bull

Kim

Lee

et al. 2023

Front. Vet. Sci.

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Bovine tetanus is a serious infectious disease of the central nervous system caused by the exotoxin produced by Clostridium tetani and is characterized by persistent tension and spasm of the rhabdomyocytes. Currently, many studies have focused on diagnosing tetanus; however, only a few studies on treatment methods have been conducted. Therefore, cattle with tetanus have been treated using symptomatic therapy. In this case, severe muscle spasticity and spasms were observed in a 9-month-old Hanwoo (Korean indigenous cattle) bull, and aspartate aminotransferase and creatine kinase levels were increased in serum biochemical tests. Clinically, bovine tetanus was strongly suspected, and metronidazole was administered orally for 5 days. To treat the intensifying bloat, a temporary rumenostomy was performed on the third day of onset, and the toxin gene (tetanospasmin) of C. tetani was amplified by polymerase chain reaction analysis from the collected ruminal fluid. Magnesium and sedatives (acepromazine) were administered for 7 days to treat muscle spasticity and spasms. Muscle spasticity and spasm markedly improved, and the bull stood up from the lateral recumbent position. On the 17th day after onset, all tetanus-related symptoms resolved and a normal diet was started. Our findings demonstrated that treatment with metronidazole, magnesium, and acepromazine was effective in the bull with tetanus.

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“…Heavy-metal ions − and organic pollutants such as pesticides and toxins accumulate at the top of the biological chain, threatening public health and inducing physiological defect to infants . However, bacteria and viruses such as Escherichia coli, Staphylococcus aureus, Clostridium tetani, human immunodeficiency virus (HIV), and hepatitis B virus (HBV) also cause severe health problems. Associated with the pathological changes of the human body, the amounts of various biomarkers also varies to abnormal levels .…”

Section: Introductionmentioning

confidence: 99%

Recent Advances on Magnetic Relaxation Switching Assay-Based Nanosensors

et al. 2017

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Magnetic relaxation switching assay (MRSw)-based nanosensors respond to the changes of transverse relaxation time (T) of water molecules resulted from the analyte-induced aggregation and disaggregation of magnetic nanoparticles (MNPs). This strategy has been widely applied to the detections of various substrates from heavy metal ions to organic pollutants, proteins, nucleic acids, bacteria and viruses, and specific cells. Compared with other nanosensors, MRSw-based nanosensors not only are free from the background interferences, signal bleaching, and quenching but also overcome light scattering from samples without pretreatments. Therefore, MRSw-based nanosensors have been developed as real-time and on-site detection platforms for environmental protection, food safety, and risk assessment. This review summarizes the latest developments of the principles, the applicable magnetic nanoparticles, and the exploited environmental and biological applications of MRSw-based nanosensors.

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Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin

Cited by 6 publications

References 8 publications

Tetanus in animals

Tetanus in animals

Successful treatment of idiopathic tetanus using metronidazole, magnesium, and acepromazine in Hanwoo (Korean indigenous cattle) yearling bull

Recent Advances on Magnetic Relaxation Switching Assay-Based Nanosensors

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