Detection of CNV in the SH3RF2 gene in chickens and its effects on growth and carcass traits

Abstract

BackgroundSH3RF2 gene was a protein-coding gene located in the quantitative trait locus associated with body weight , and its deletion was positively correlated with body weight in chickens.ResultsIn the present study, the SH3RF2 genotype of the “Gushi ×Anka” F2 resource population , as well as local chickens and commercial layers and broilers in China was detected , and the relationship between the genotype and chicken production traits was further studied by molecular biology technology. The results showed that the local chickens and commercial layers were all homozygous for the wild-type allele. In commercial broiler breeds, deletion mutation individuals were detected in all commercial broiler breeds except Hubbard breeds.In addition , 798 individuals in the F2 resource group were used to analyze the effects of different genotypes (DD\ ID\ II) on chicken production traits. The results showed that the mutation was associated with 2 , 6 , 10 , and 12-week body weight and a significant correllation with 8-week breast bone length (P<0.05);The mutation was significantly correlated with 8-week body weight and 4-week breast bone length (P<0.01).Additionally , evisceration weight , legs muscle weight , carcass weight and other significant correlations (P<0.05) and breast muscle weight and gizzard weight were significantly correlated (P<0.01).ConclusionsThe results of this study indicated that the copy number variation of the SH3RF2 gene contributed to the growth and weight gain of chickens.