Detection of CoHV-1 by loop-mediated amplification (LAMP). Application of LAMP for CoHV-1 incidence monitoring in domestic pigeons

Abstract

The incidence of Columbid herpesvirus-1 (CoHV-1) in pigeons was examined for the first time by loop-mediated isothermal amplification (LAMP). The study showed the presence of CoHV-1 in 8 of 42 examined domestic pigeons. LAMP technique developed within this study may be used by not well-equipped veterinary laboratories.

Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species

“…In recent years, PCR and qPCR based methods for detection of HRF genus Neosartorya 19 , genus Thermoascus 38 , genus Byssochalmys and Hamigera 18 and species Talaromyces flavus 20 . Due to emergence of isothermal techniques such as LAMP, researchers developed methods to utilize these techniques to detect microorganisms and viruses important for the animal and plant food processing and consumer health 30,39–44 and in general public health sensu largo 45–51 . However, LAMP methods for detection of fungi are scarce, while there are no isothermal methods concentrated on detecting any of HRF, yet alone Talaromyces flavus .…”