Detection of colistin resistance mcr-1 gene in Salmonella enterica serovar Rissen isolated from mussels, Spain, 2012­ to 2016

Lozano-León, Antonio; García-Omil, Carlos; Dalama, Jacobo; Rodriguez‐Souto, Rafael R.; Martı́nez-Urtaza, Jaime; González-Escalona, Narjol

doi:10.2807/1560-7917.es.2019.24.16.1900200

Cited by 26 publications

(25 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Salmonella Agona ST13 was the most prevalent serovar isolated from chicken meat in Sri Lanka [ 33 ], and a Salmonella Agona strain isolated from chicken meat in China possessed a T57S substitution in ParC and carried qnrS [ 34 ]. Salmonella Rissen ST469 was isolated from ready-to-eat mussels in Spain and pork products in Portugal [ 35 , 36 ]. Moreover, ST469 was the third most predominant ST isolated from pork samples in China and nearly one-third of the ST469 isolates were resistant to ciprofloxacin [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

et al. 2021

View full text Add to dashboard Cite

Non-typhoidal salmonellosis remains a pressing public health problem worldwide. Quinolones, particularly fluoroquinolones, are widely used to treat various infections, including non-typhoidal salmonellosis, which can be a serious illness. The emergence of fluoroquinolone-resistant Salmonella has resulted in treatment failure and high mortality rates. In this study, we estimated the presence of plasmid-mediated quinolone resistance (PMQR) genes in Salmonella enterica isolated from human salmonellosis patients in South Korea from 2016 to 2019. We evaluated the association of these genes with fluoroquinolone susceptibility. Antimicrobial susceptibility tests for Salmonella isolates were performed using the Vitek II system, and the minimum inhibitory concentrations (MIC) of ciprofloxacin and levofloxacin were determined using the E-test method. Plasmid-mediated quinolone resistance (PMQR) genes were detected by PCR amplification and quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were analyzed following Sanger sequencing of the PCR products. Thirty-four Salmonella strains with reduced susceptibility to fluoroquinolones (ciprofloxacin MIC ≥ 0.125 µg/mL and levofloxacin MIC ≥ 0.25 µg/mL) were selected from 208 human clinical Salmonella isolates. Among them, 22 Salmonella strains harbored one PMQR gene (qnrA, qnrB, or qnrS), and three Salmonella strains carried two PMQR genes (qnrS and aac(6′)-Ib-cr or qnrA and qnrB). qnrS was the most common PMQR gene. Serotyping revealed that Salmonella 4,[5]12:i:- (32.4%, 11/34) and Salmonella Typhimurium (29.4%, 10/34) were the two most predominant serovars, and Multi-locus sequence typing (MLST) showed that ST19 and ST34 were the most frequent sequence types. In conclusion, qnr gene-positive Salmonella 4,[5],12:i:- and Salmonella Typhimurium were the main serovars responsible for reduced susceptibility to fluoroquinolones. Therefore, our findings suggest that PMQR-positive Salmonella strains, which can be isolated from various samples including human, food, and the environment, should be carefully monitored.

show abstract

Section: Resultsmentioning

confidence: 99%

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In Vietnam, mcr-1 was detected in one extended-spectrum beta-lactamase (ESBL)-producing E. coli isolated from striped catfish grown in ponds [ 25 , 26 ]. A recent study from Spain reported mcr-1 in Salmonella enterica serovar Rissen isolated from mussels [ 27 ]. However, beyond these few studies on farmed fish in China and Vietnam and on mussels in Spain [ 23 , 24 , 25 , 26 , 27 ], data on the occurrence of mcr in aquaculture have been limited.…”

Section: Introductionmentioning

confidence: 99%

“…A recent study from Spain reported mcr-1 in Salmonella enterica serovar Rissen isolated from mussels [ 27 ]. However, beyond these few studies on farmed fish in China and Vietnam and on mussels in Spain [ 23 , 24 , 25 , 26 , 27 ], data on the occurrence of mcr in aquaculture have been limited. Therefore, it is important to investigate colistin resistance and mcr in aquaculture globally, especially in developing countries with limited infrastructure and documented challenges in pollution.…”

Section: Introductionmentioning

confidence: 99%

The Mobile Colistin Resistance Gene, mcr-1.1, Is Carried on IncX4 Plasmids in Multidrug Resistant E. coli Isolated from Rainbow Trout Aquaculture

et al. 2020

View full text Add to dashboard Cite

Colistin, a last resort antibiotic, is important for controlling infections with carbapenem-resistant Enterobacteriaceae. The recent emergence of mobile-colistin-resistance (mcr) genes has threatened the effectiveness of colistin. Aquaculture is hypothesized to be a major contributor to the evolution and dissemination of mcr. However, data on mcr in aquaculture are limited. Here, the occurrence of mcr-1 was assessed in Rainbow Trout in Lebanon, a country with developing antimicrobial stewardship and an established use of colistin for medical and farming purposes. mcr-1 was detected in 5 Escherichia coli isolated from fish guts. The isolates were classified as multidrug-resistant and their colistin minimum inhibitory concentration ranged between 16 and 32 μg/mL. Whole genome sequencing analysis showed that mcr-1 was carried on transmissible IncX4 plasmids and that the isolates harbored more than 14 antibiotic resistance genes. The isolates belonged to ST48 and ST101, which have been associated with mcr and can occur in humans and fish. The mcr-1-positive E. coli persisted in 6-day biofilms, but there was a potential fitness cost. Given the status of infrastructure in Lebanon, there is a high potential for the dissemination of mcr via aquatic environments. Urgent actions are needed to control mcr and to enhance antimicrobial stewardship in Lebanon.

show abstract

“…Strains of Salmonella are important pathogens of concern in food safety, as they are frequently transmitted between agricultural animals, food, and humans ( Foley and Lynne, 2008 ); the pathogens often cause gastroenteritis, in some cases severe, and are responsible for >600,000 deaths annually ( Lokken et al, 2016 ). Increasing antimicrobial resistance in Salmonella species is considered an important public health concern of the 21st century ( Lozano-Leon et al, 2019 ). Salmonella strains that acquire multidrug resistance genes would be difficult to treat and would result in an even higher number of cases with severe morbidity and high frequency of mortality.…”

Section: Introductionmentioning

confidence: 99%

Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China

Fan

Zhang

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Salmonella is an important food pathogen that can cause severe gastroenteritis with more than 600,000 deaths globally every year. Colistin (COL), a last-resort antibiotic, is ineffective in bacteria that carry a functional mcr-1 gene, which is often spread by conjugative plasmids. Our work aimed to understand the prevalence of the mcr-1 gene in clinical isolates of Salmonella, as the frequency of occurrence of the mcr-1 gene is increasing globally. Therefore, we analyzed 689 clinical strains, that were isolated between 2009 and late 2018. The mcr-1 gene was found in six strains, which we analyzed in detail by whole genome sequencing and antibiotic susceptibility testing, while we also provide the clinical information on the patients suffering from an infection. The genomic analysis revealed that five strains had plasmid-encoded mcr-1 gene located in four IncHI2 plasmids and one IncI2 plasmid, while one strain had the chromosomal mcr-1 gene originated from plasmid. Surprisingly, in two strains the mcr-1 genes were inactive due to disruption by insertion sequences (ISs): ISApl1 and ISVsa5. A detailed analysis of the plasmids revealed a multitude of ISs, most commonly IS26. The IS contained genes that meditate broad resistance toward most antibiotics underlining their importance of the mobile elements, also with respect to the spread of the mcr-1 gene. Our study revealed potential reservoirs for the transmission of COL resistance and offers insights into the evolution of the mcr-1 gene in Salmonella.

show abstract

Detection of colistin resistance mcr-1 gene in Salmonella enterica serovar Rissen isolated from mussels, Spain, 2012 to 2016

Cited by 26 publications

References 16 publications

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

The Mobile Colistin Resistance Gene, mcr-1.1, Is Carried on IncX4 Plasmids in Multidrug Resistant E. coli Isolated from Rainbow Trout Aquaculture

Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China

Contact Info

Product

Resources

About

Detection of colistin resistance mcr-1 gene in Salmonella enterica serovar Rissen isolated from mussels, Spain, 2012­ to 2016

Cited by 26 publications

References 16 publications

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

Presence of plasmid-mediated quinolone resistance (PMQR) genes in non-typhoidal Salmonella strains with reduced susceptibility to fluoroquinolones isolated from human salmonellosis in Gyeonggi-do, South Korea from 2016 to 2019

The Mobile Colistin Resistance Gene, mcr-1.1, Is Carried on IncX4 Plasmids in Multidrug Resistant E. coli Isolated from Rainbow Trout Aquaculture

Plasmid Dynamics of mcr-1-Positive Salmonella spp. in a General Hospital in China

Contact Info

Product

Resources

About

Detection of colistin resistance mcr-1 gene in Salmonella enterica serovar Rissen isolated from mussels, Spain, 2012 to 2016