Detection of Corynebacterium kutscheri from the Oral Cavity of Rats.

Amao, Hiromi; Akimoto, Toshio; Komukai, Yumi; Sawada, Takuo; Saito, Manabu; Takahashi, Kazuaki W.

doi:10.1538/expanim.51.99

Cited by 15 publications

(9 citation statements)

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“…C. kutscheri has primarily been reported to be a common bacterium on the mucous membranes of rodents including the gastrointestinal tract [ 53 ]. Interestingly, C. kutscheri was more plentiful in the intestinal content of healthy piglets than in that of diarrhoeic piglets, irrespective of gut section.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the bacterial gut microbiota of piglets suffering from new neonatal porcine diarrhoea

et al. 2015

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BackgroundIn recent years, new neonatal porcine diarrhoea (NNPD) of unknown aetiology has emerged in Denmark. NNPD affects piglets during the first week of life and results in impaired welfare, decreased weight gain, and in the worst-case scenario death. Commonly used preventative interventions such as vaccination or treatment with antibiotics, have a limited effect on NNPD. Previous studies have investigated the clinical manifestations, histopathology, and to some extent, microbiological findings; however, these studies were either inconclusive or suggested that Enterococci, possibly in interaction with Escherichia coli, contribute to the aetiology of NNPD. This study examined ileal and colonic luminal contents of 50 control piglets and 52 NNPD piglets by means of the qPCR-based Gut Microbiotassay and 16 samples by 454 sequencing to study the composition of the bacterial gut microbiota in relation to NNPD.ResultsNNPD was associated with a diminished quantity of bacteria from the phyla Actinobacteria and Firmicutes while genus Enterococcus was more than 24 times more abundant in diarrhoeic piglets. The number of bacteria from the phylum Fusobacteria was also doubled in piglets suffering from diarrhoea. With increasing age, the gut microbiota of NNPD affected piglet and control piglets became more diverse. Independent of diarrhoeic status, piglets from first parity sows (gilts) possessed significantly more bacteria from family Enterobacteriaceae and species E. coli, and fewer bacteria from phylum Firmicutes. Piglets born to gilts had 25 times higher odds of having NNPD compared with piglets born to multiparous sows. Finally, the co-occurrence of genus Enterococcus and species E. coli contributed to the risk of having NNPD.ConclusionThe results of this study support previous findings that points towards genus Enterococcus and species E. coli to be involved in the pathogenesis of NNPD. Moreover, the results indicate that NNPD is associated with a disturbed bacterial composition and larger variation between the diarrhoeic piglets.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-015-0419-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Characterization of the bacterial gut microbiota of piglets suffering from new neonatal porcine diarrhoea

et al. 2015

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“…However, the organism was not detected in direct impression cultures of faeces from two mice infected with low numbers of the organism (below 10 3.80 CFU/g). We recommend cultures of homogenized faecal pellets in HIB in combination with cultures of oral swabs for the detection of C. kutscheri based on the results of the present study and our previous study (Amao et al 2002), in which oral cavity swabs from rats were cultured on FNC agar and were found to be useful for the detection of subclinical infections with C. kutscheri.…”

Section: Discussionmentioning

confidence: 70%

Detection of Corynebacterium kutscheri in the faeces of subclinically infected mice

Amao¹,

Moriguchi²,

Komukai³

et al. 2008

Lab Anim

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SummaryMice were infected experimentally and subclinically with Corynebacterium kutscheri to recover the organism from mice faeces. The faeces were then cultured using selective furazolidone-nalidixic acid-colimycin agar. The number of C. kutscheri per gram of fresh faeces varied from mouse to mouse, but once established in the intestine, the organism was excreted in the faeces for at least five months. Viable bacteria were detected in most of the faecal samples, including those stored in the animal room for five days. The number of organisms in the stored faeces decreased gradually but did not differ significantly from those in the fresh faeces until they had been stored for more than three days. Many infected mice excreted between 10 4.77 and 10 5.37 colony forming units (CFU) of C. kutscheri per day in their faeces, and one mouse even excreted 10 3.74 CFU at eight weeks postinfection. These values showed little daily variation. Our present study showed that subclinically infected mice discharged the organism continuously and persistently in their faeces. Therefore, faecal samples would be useful for monitoring infection with C. kutscheri in living mice in a manner that is not stressful for the animals.

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“…Diagnosis In infected colonies, C. kutscheri will typically cause latent infections and may be detected by PCR or cultured from submaxillary (cervical) lymph nodes, oropharynx and nasopharynx, middle ears, and preputial glands of feces (Amao et al, 1995(Amao et al, , 2002(Amao et al, , 2008. Latent infections may be triggered to become clinical by a variety of stressors that can cause immunosuppression in the host.…”

Section: Pseudotuberculosismentioning

confidence: 99%