Detection of Crimean-Congo hemorrhagic fever virus in ticks collected from South Russia

Цапко, Н. В.; Волынкина, А. С.; Evchenko, Anna Yu.; Lisitskaya, Yana V; Shaposhnikova, Ludmila I

doi:10.1016/j.ttbdis.2021.101890

Cited by 5 publications

(6 citation statements)

References 19 publications

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“…Firstly, the sample size of 70 sequences (24 for S segment, 23 for M segment, and 23 for L segment) of CCHFV isolates from different representatives of the Hyalomma and Rhipicephalus genera may not be sufficient to provide robust conclusions. Additionally, there are limitations in the amount of full CCHFV genome sequences isolated from Rhipicephalus and the lack of CCHFV sequences from Dermacentor species, despite experimental evidence suggesting their potential involvement in virus transmission and the frequent detection of viral RNA in these species [11][12][13][14][15][16]18,19,[44][45][46]. Thus, generating full genome sequences of CCHFV strains isolated from Dermacentor ticks could provide valuable insights into the virus's adaptation to additional tick species.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, in genus Dermacentor , D . marginatus Sulzer, 1776 has tested positive for CCHFV in Turkey, Russia, and Spain [ 12 , 14 , 15 , 17 , 18 ], highlighting its potential as a vector for the virus. Despite the detection of CCHFV within D .…”

Section: Introductionmentioning

confidence: 99%

“…Ticks from other genera of Ixodidae, including Rhipicephalus and Dermacentor, are also capable of transmitting CCHFV. Rhipicephalus ticks, such as R. bursa Koch, 1844, and R. turanicus Pomerantsev, 1936 have been identified as carriers of CCHFV in regions spanning Albania, Turkey, Greece, and Russia [12][13][14][15][16]. Similarly, in genus Dermacentor, D. marginatus Sulzer, 1776 has tested positive for CCHFV in Turkey, Russia, and Spain [12,14,15,17,18], highlighting its potential as a vector for the virus.…”

Section: Introductionmentioning

confidence: 99%

“…Rhipicephalus ticks, such as R. bursa Koch, 1844, and R. turanicus Pomerantsev, 1936 have been identified as carriers of CCHFV in regions spanning Albania, Turkey, Greece, and Russia [12][13][14][15][16]. Similarly, in genus Dermacentor, D. marginatus Sulzer, 1776 has tested positive for CCHFV in Turkey, Russia, and Spain [12,14,15,17,18], highlighting its potential as a vector for the virus. Despite the detection of CCHFV within D. marginatus eggs and its confirmed competency as a tick vector for the virus in laboratory studies, questions remain regarding their natural vectorial capacity within the enzootic cycle of the virus [11,19].…”

Section: Introductionmentioning

confidence: 99%

“…While Hyalomma species are the primary virus reservoirs and vectors for CCHFV, there is experimental evidence suggesting the potential involvement of ticks from the Rhipicephalus genus in its transmission; however, concrete evidence supporting their significant role in viral maintenance and natural transmission remains inconclusive [19]. Recent studies have detected CCHFV antigen within a Rhipicephalus species, along with viral RNA in different Rhipicephalus species across Albania, Kosovo, Greece, Turkey, and Iran [12][13][14][15][16][44][45][46]. Notably, this highlights the exclusive presence of CCHFV strains belonging to Europe 2 (clade VI) within R. bursa species, indicating their potential role as vectors of the virus [13].…”

Section: Introductionmentioning

confidence: 99%

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Genetic background of adaptation of Crimean-Congo haemorrhagic fever virus to the different tick hosts

S. Celina,

Černý

2024

PLoS ONE

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Crimean-Congo haemorrhagic fever orthonairovirus (CCHFV) is a negative-sense, single-stranded RNA virus with a segmented genome and the causative agent of a severe Crimean-Congo haemorrhagic fever (CCHF) disease. The virus is transmitted mainly by tick species in Hyalomma genus but other ticks such as representatives of genera Dermacentor and Rhipicephalus may also be involved in virus life cycle. To improve our understanding of CCHFV adaptation to its tick species, we compared nucleotide composition and codon usage patterns among the all CCHFV strains i) which sequences and other metadata as locality of collection and date of isolation are available in GenBank and ii) which were isolated from in-field collected tick species. These criteria fulfilled 70 sequences (24 coding for S, 23 for M, and 23 for L segment) of virus isolates originating from different representatives of Hyalomma and Rhipicephalus genera. Phylogenetic analyses confirmed that Hyalomma- and Rhipicephalus-originating CCHFV isolates belong to phylogenetically distinct CCHFV clades. Analyses of nucleotide composition among the Hyalomma- and Rhipicephalus-originating CCHFV isolates also showed significant differences, mainly in nucleotides located at the 3rd codon positions indicating changes in codon usage among these lineages. Analyses of codon adaptation index (CAI), effective number of codons (ENC), and other codon usage statistics revealed significant differences between Hyalomma- and Rhipicephalus-isolated CCHFV strains. Despite both sets of strains displayed a higher adaptation to use codons that are preferred by Hyalomma ticks than Rhipicephalus ticks, there were distinct codon usage preferences observed between the two tick species. These findings suggest that over the course of its long co-evolution with tick vectors, CCHFV has optimized its codon usage to efficiently utilize translational resources of Hyalomma species.

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