Detection of Cronobacter species in powdered infant formula by probe-magnetic separation PCR

Xu, Feng; P, Li; Ming, Xing; Yang, Dong; Xu, Hengyi; Wu, Xiaoli; Shah, Nagendra P.; Wei, Hua

doi:10.3168/jds.2014-8287

Cited by 18 publications

(14 citation statements)

References 32 publications

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“…Cronobacter species have been identified as a contaminant of powdered milk formulas, with sporadic outbreaks linked to necrotizing enterocolitis, bacteremia, and meningitis. 7778 Bacteria can also colonize unlikely sources. Burkholderia cepacia and Enterobacter cloacae have the ability to hydrolyze, render inactive, and proliferate in parabens, which are esters of para-hydroxybenzoic acid that are usually antimicrobial and used as preservatives in ultrasound gel (implicated in a Burkholderia cepacia outbreak in a NICU).…”

Section: The Microbiota and Disease Risk In Extremely Preterm Infantsmentioning

confidence: 99%

The Microbiota of the Extremely Preterm Infant

Underwood

Sohn

2017

Clinics in Perinatology

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Colonization of the extremely preterm infant's gastrointestinal tract and skin begins in utero and is influenced by a variety of factors, the most important including gestational age and environmental exposures. The composition of the intestinal and skin microbiota influences the developing innate and adaptive immune responses with short-term and long-term consequences including altered risks for developing necrotizing enterocolitis, sepsis, and a wide variety of microbe-related diseases of children and adults. Alteration of the composition of the microbiota to decrease disease risk is particularly appealing for this ultra-high-risk cohort that is brand new from an evolutionary standpoint.

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Section: The Microbiota and Disease Risk In Extremely Preterm Infantsmentioning

confidence: 99%

The Microbiota of the Extremely Preterm Infant

Underwood

Sohn

2017

Clinics in Perinatology

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“…The sensitivity of this HRM assay in PIF without enrichment was comparable to that of PCR‐ELISA assay, which was 1.06 × 10 3 cfu/mL as previously reported (Xu et al . 2014a). The HRM assay was able to detect undesiccated Cronobacter in PIF at 0.25 cfu/25 g following a 12‐h enrichment, which is equivalent to the ESIA testing (Table ) and comparable to a multiplex real‐time PCR assay reported by Hyeon et al .…”

Section: Discussionmentioning

confidence: 99%

Simultaneous detection and identification of pathogenic Cronobacter species by high‐resolution melting analysis in powdered infant formulas

et al. 2017

Int J of Dairy Tech

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A combination method of real-time PCR and high-resolution melting (HRM) analysis for the rapid detection and specific classification of six pathogenic Cronobacter species based on gene cgcA was developed. HRM profiles with distinct T m (melting temperature) peaks were consistently obtained with each species, represented by a single peak ranging from 86.02 to 86.80°C. The detection limit ranged from 0.1 to 1 pg per reaction. Desiccated Cronobacter sakazakii and Cronobacter muytjensii of 2 cfu/25 g were successfully detected after 4-week storage at room temperature. The newly developed method provided a molecular tool for direct detection and simultaneous identification of pathogenic Cronobacter species in powdered infant formulae.

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“…However, due to low Cronobacter numbers in contaminated PIF [113] and the inhibiting effect of food components, enrichment is still necessary in order to increase cell concentrations to the detection limit. To improve detection efficiency, methods that enrich detection targets, such as immunomagnetic separation [91] and probemagnetic separation [114] , should be developed.…”

Section: Detection Of Cronobactermentioning

confidence: 99%

Cronobacter spp., foodborne pathogens threatening neonates and infants

Chen¹,

Zhu²,

Qin³

et al. 2018

Front. Agr. Sci. Eng.

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Cronobacter spp. (formerly Enterobacter sakazakii) are special foodborne pathogens. Cronobacter infection can cause necrotizing enterocolitis, sepsis and meningitis in all age groups, especially neonates and infants, with a high fatality of up to 80%, although the infection is rare. Outbreaks of Cronobacter infection are epidemiologically proven to be associated with contaminated powdered infant formula (PIF). Cronobacter spp. can resist dry environments and survive for a long period in food with low water activity. Therefore, Cronobacter spp. have become serious pathogens of neonates and infants, as well as in the dairy industry. In this review, we present the taxonomy, pathogenesis, resistance, detection and control of Cronobacter spp.

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Detection of Cronobacter species in powdered infant formula by probe-magnetic separation PCR

Cited by 18 publications

References 32 publications

The Microbiota of the Extremely Preterm Infant

The Microbiota of the Extremely Preterm Infant

Simultaneous detection and identification of pathogenic Cronobacter species by high‐resolution melting analysis in powdered infant formulas

Cronobacter spp., foodborne pathogens threatening neonates and infants

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