Detection of CTX-M-15 beta-lactamases in Enterobacteriaceae causing hospital- and community-acquired urinary tract infections as early as 2004, in Dar es Salaam, Tanzania

Manyahi, Joel; Moyo, Sabrina John; Tellevik, Marit Gjerde; Ndugulile, Faustine; Urassa, Willy; Blomberg, Björn; Langeland, Nina

doi:10.1186/s12879-017-2395-8

Cited by 37 publications

(41 citation statements)

References 26 publications

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“…26 In contrast to our study, a lower ESβL UTI prevalence was detected in other studies, in Dar es Salaam hospital, Tanzania (over 6 months period), and Marrakech University Hospital, Morocco (27% and 4.5%, respectively), between 2010 and 2012. 6,27 In Saudi Arabia and Tanzania, a higher frequency of ESβL-induced UTIs was found to be hospitalacquired compared with community-acquired infections (67% and 64%, respectively). 6,28 The high frequency of ESβL, especially among community-acquired UTIs, in our study is Extended-spectrum β-lactamase isolates were extremely resistant to cefepime and amoxicillin-clavulanic acid, and susceptible to carbapenems.…”

Section: Discussionmentioning

confidence: 99%

“…3 The spread of extended-spectrum β-lactamase (ESβL) Enterobacteriaceae UTI is increasing worldwide. 4,5 Although most of the ESβL producers are isolated from hospitalacquired UTIs, 6,7 a higher rate was screened by Nisha et al 8 and Djuikoue et al 9 from community-acquired UTIs. Escherichia coli and Klebsiella pneumoniae are the most predominant ESβL Enterobacteriaceae in the clinical setting; this has contributed to their beneficial value in estimating the prevalence of ESβL producers among different infections.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of Extended-Spectrum β-Lactamase Enterobacteriaceae Isolated from Egyptian Patients with Community- and Hospital-Acquired Urinary Tract Infection

Shash

Elshimy

Soliman

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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Extended-spectrum β-lactamases (ESβLs) pose a serious problem in the treatment of urinary tract infections (UTIs). The ESβL-producing organism is an expanding global health problem. Therefore, screening for ESβL, detection of their drug-resistance pattern, and molecular characterization should be a continuous process. The present study was performed to determine the antibiotic resistance profile and the genetic characterization of ESβL isolates from hospital-and community-acquired UTIs. Two hundred fifty Enterobacteriaceae isolates were obtained from urine samples of outpatient clinic attendants and hospitalized patients at Kasr Al-Aini Hospital. By phenotypic screening tests, 100 ESβL isolates were detected among the studied groups. Furthermore, detection of beta-lactamase (bla) cefotaxime (CTX)-M, sulfhydryl variable, and temoneira ESβL genes was investigated by polymerase chain reaction. A subset of 25 CTX-M-positive isolates was further identified by gene sequencing technology. Among the 100 ESβL isolates, 66% were Escherichia coli and 34% were Klebsiella spp. There was no statistical difference in the prevalence of ESβL Enterobacteriaceae in community-acquired versus hospital-acquired UTIs. The susceptibility of all ESβL isolates to carbapenems was the most prevalent finding. In addition, all ESβL E. coli isolates were susceptible to fosfomycin, whereas all community-acquired ESβL isolates were susceptible to nitrofurantoin. A total of 98% of the ESβL isolates harbored bla-CTX-M genes, with CTX-M-15 being the most prevalent. It could be concluded that ESβL production is present at a high rate among Egyptian patients with hospital-and community-acquired UTI. The high prevalence of bla-CTX-M may suggest it as a candidate for molecular screening of ESβL.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Extended-Spectrum β-Lactamase Enterobacteriaceae Isolated from Egyptian Patients with Community- and Hospital-Acquired Urinary Tract Infection

Shash

Elshimy

Soliman

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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“…In all countries the origin was faecal (except Tunisia, with mastitis also). All types of descriptions presented in these 5 countries for E-ESBL in cattle also present reports in humans (Abbassi et al, 2008;Iroha et al, 2012;Manyahi et al, 2017;Ouedraogo et al, 2016).…”

Section: Africamentioning

confidence: 97%

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

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Food producing animal is a global challenge in terms of antimicrobial resistance spread. Extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae are relevant opportunistic pathogens that may spread in many ecological niches of the One Health approach as human, animal and environment due to intestinal selection of antimicrobial resistant commensals in food production animals. Cattle production is a relevant ecological niche for selection of commensal bacteria with antimicrobial resistance from microbiota. Enterobacteriaceae show importance in terms of circulation of resistant-bacteria and antimicrobial resistance genes via food chain creating a resistance reservoir, setting up a threat for colonization of humans and consequent health risk. ESBL-producing Enterobacteriaceae are a threat in terms of human health responsible for life threatening outbreaks and silent enteric colonization of community populations namely the elder population. Food associated colonization is a risk difficult to handle and control. In a time of globalization of food trading, population intestinal colonization is a mirror of food production and in that sense this work aims to make a picture of ESBL-producing Enterobacteriaceae in animal production for food over the world in order to make some light in this reality of selection of resistant threats in food producing animal.

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“…Injudicious use of ceftriaxone may contribute to the high burden of AMR, including nosocomial infections with extended spectrum beta lactamase (ESBL)-producing Enterobacteriaceae, and complicate empirical treatment of severe bacterial infections. In view of the high prevalence of colonization and infections with ESBL positive Enterobacteriaceae in Tanzanian hospitals [21][22][23][24], increasing use of carbapenems in the future can be anticipated. In an attempt to limit empiric carbapenem use, the Tanzanian National Health Insurance Fund (NHIF) implemented a policy in July 2016 that limits carbapenem coverage to infections with a positive bacterial culture and sensitivity [25].…”

Section: Discussionmentioning

confidence: 99%

Point Prevalence Survey of antimicrobial use in three hospitals in North-eastern Tanzania

Horumpende

Mshana

Mouw

et al. 2020

Preprint

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Background: Antimicrobial resistance (AMR) is one of the most urgent global health threats with low-resource countries being disproportionately affected. Targeted interventions require insight in antibiotic prescription practices. A point prevalence survey (PPS) is a well-known tool to get insight in antibiotic dispensing practices in hospitals and identify areas for improvement. Here, we describe the results of a PPS performed in a tertiary, regional and district hospital in Kilimanjaro region in Tanzania. Methods: A PPS was performed in the Kilimanjaro Christian Medical Centre (KCMC; tertiary hospital), Mawenzi (regional) and St. Joseph (district) hospital in November and December 2016. Antibiotic use in all patients admitted more than 24 hours and those undergoing surgery was recorded. All clinical wards were included except the pediatrics. Data from a single ward were collected on the same day. Results: A total of 399 patients were included in the PPS: 232 patients from KCMC, 94 from Mawenzi hospital and 73 patients from St. Joseph hospital. Overall prevalence of antibiotic use was 44.0%: 38% in KCMC, 59% in Mawenzi and 63% in St. Joseph. Ceftriaxone (n=94, 29.8%), metronidazole (n=79, 23.9%) and other antibiotics belonging to the penicillin class (n=89, 28.3%) were most commonly prescribed. Antibiotics prescribed for surgical prophylaxis were continued for more than 3 days in 57% of cases.Conclusion: Our study shows a rate of broad-spectrum antibiotic use in Tanzanian hospitals and prolonged surgical antibiotic prophylaxis being a common practice. PPS is an important tool to improve future antibiotic use in Tanzania hospitals.

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Detection of CTX-M-15 beta-lactamases in Enterobacteriaceae causing hospital- and community-acquired urinary tract infections as early as 2004, in Dar es Salaam, Tanzania

Cited by 37 publications

References 26 publications

Molecular Characterization of Extended-Spectrum β-Lactamase Enterobacteriaceae Isolated from Egyptian Patients with Community- and Hospital-Acquired Urinary Tract Infection

Molecular Characterization of Extended-Spectrum β-Lactamase Enterobacteriaceae Isolated from Egyptian Patients with Community- and Hospital-Acquired Urinary Tract Infection

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Point Prevalence Survey of antimicrobial use in three hospitals in North-eastern Tanzania

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