Detection of Cyclospora cayetanensis, Echinococcus multilocularis, Toxocara spp. and microsporidia in fresh produce using molecular methods: – A review

Bartosova, Barbora; Koudela, Břetislav; Slaná, Iva

doi:10.1016/j.fawpar.2021.e00124

Cited by 7 publications

(3 citation statements)

References 83 publications

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Section: Detection Methodsmentioning

confidence: 99%

“…A recent review focused on the molecular methods for the detection of C. cayetanensis in fresh produce [ 87 ]. The detection of C. cayetanensis in fresh produce has been performed using several methods including fluorescence microscopy, conventional PCR, and TaqMan probe-based qPCR [ 87 , 88 , 89 ]. PCR-based methods for the detection of C. cayetanensis in produce have traditionally been performed using primers targeting the 18S rRNA gene, internal transcribed spacer 2 (ITS 2), or the hsp70 gene [ 87 ].…”

Section: Detection Methodsmentioning

confidence: 99%

“…The detection of C. cayetanensis in fresh produce has been performed using several methods including fluorescence microscopy, conventional PCR, and TaqMan probe-based qPCR [ 87 , 88 , 89 ]. PCR-based methods for the detection of C. cayetanensis in produce have traditionally been performed using primers targeting the 18S rRNA gene, internal transcribed spacer 2 (ITS 2), or the hsp70 gene [ 87 ]. However, new targets have recently been developed for screening produce samples for the presence of C. cayetanensis [ 82 , 88 , 89 , 90 ].…”

Section: Detection Methodsmentioning

confidence: 99%

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Cyclospora cayetanensis: A Perspective (2020–2023) with Emphasis on Epidemiology and Detection Methods

Almeria,

Chacin-Bonilla,

Maloney

et al. 2023

Microorganisms

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Cyclospora cayetanensis infections are prevalent worldwide, and the parasite has become a major public health and food safety concern. Although important efforts have been dedicated to advance toward preventing and reducing incidences of cyclosporiasis, there are still several knowledge gaps that hamper the implementation of effective measures to prevent the contamination of produce and water with Cyclospora oocysts. Some of these data gaps can be attributed to the fact that access to oocysts is a limiting factor in C. cayetanensis research. There are no animal models or in vivo or in vitro culture systems to propagate the oocysts needed to facilitate C. cayetanensis research. Thus, researchers must rely upon limited supplies of oocysts obtained from naturally infected human patients considerably restricting what can be learnt about this parasite. Despite the limited supply of C. cayetanensis oocysts, several important advances have happened in the past 3 years. Great progress has been made in the Cyclospora field in the areas of molecular characterization of strains and species, generation of genomes, and development of novel detection methods. This comprehensive perspective summarizes research published from 2020 to 2023 and evaluates what we have learnt and identifies those aspects in which further research is needed.

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