Detection of Cymbidium mosaic virus in Protocorm-Like Bodies in Dendrobium Sonia using One-Step RT-PCR

Tissue disinfection for preparation of Dendrobium in vitro culture

“…Khentry et al (2006a) observed, in 14 Dendrobium cut flowers propagated by cuttings, that the rate of infection by CymMV ranged from 25-100% with a mean of 65.8% of samples infected, and in 29 cultivars propagated by tissue culture, a range of 0 to 100% of plantlets were infected, depending on the cultivar, with a mean of 18.6% of all samples infected. CymMV was also detected in PLBs of Dendrobium 'Sonia' obtained from tissue culture laboratories in Thailand using RT-PCR (in 78% of samples) and ELISA (in 22% of samples), showing that RT-PCR was more sensitive at detecting systemic viruses in orchids (Khentry et al 2007). The use of multiplex RT-PCR could be used for simultaneous detection of CymMV, ORSV and Orchid fleck virus (OFV) in Dendrobium and another orchid genus (Ali et al 2014, Kim andChoi 2015).…”

“…Using meristem (0.1 to 1 mm) culture of Mokara Char Kuan 'Pink', Lim et al (1993) observed that regenerated plantlets from the culture of larger meristems remained infected by CymMV and ORSV while TLCs of infected plantlets and PLBs, when treated with ribavirin, were free of these viruses. Interestingly, few papers described or show advances in studies of these microorganisms, although contamination problems continue to be observed in commercial laboratories and commercial orchid nurseries (Khentry et al 2006a(Khentry et al , 2006b(Khentry et al , 2007 Fig. 1A, B, I-U).…”