Detection of Cyprinid Herpesvirus 1 <scp>DNA</scp> in cutaneous squamous cell carcinoma of koi carp (<i>Cyprinus carpio</i>)

Sirri, Rubina; Ciulli, Sara; Barbè, Tim; Volpe, Enrico; Lazzari, Maurizio; Franceschini, Valeria; Errani, Francesca; Sarli, Giuseppe; Mandrioli, Luciana

doi:10.1111/vde.12482

Cited by 16 publications

(17 citation statements)

References 31 publications

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“…Although the virological agent of the disease in Bream has not yet been identified, an association between these tumors and the presence of viruses has been demonstrated in cyprinids (Sirri et al. ). This pathology could be related to adverse and polluted environmental conditions and algal oncogenic toxins (Chukalova et al.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Study of Four Fish Tumors

et al. 2019

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The present study supports the usefulness of ancillary techniques, such as immunohistochemistry, as a valid diagnostic tool in the field of fish oncology. The immunohistochemical patterns observed in four neoplasms on four individual teleosts belonging to different species are described. Cytokeratin, vimentin, actin, S100, calretinin, and Melan-A antibodies were used. Diagnoses of papilloma in a Bream Abramis brama, fibroma in a Sand Steenbras Lithognathus mormyrus, schwannoma in a Crucian Carp Carassius carassius, and melanoma in a spontaneously inbred Xiphophorus hybrid were made. Diagnosis of tumors in fish is not always easy to carry out, and the tool provided by antibodies used on mammalian tissue is essential for obtaining definitive, unambiguous, and inexpensive identification.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical Study of Four Fish Tumors

et al. 2019

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“…CyHV-1 is a well-known viral pathogen of common carp causing benign epidermal proliferations (Sano et al, 1985), and recently it has also been detected in cutaneous squamous cell carcinoma of koi carp (Sirri et al, 2018). According to current knowledge, herpesviruses have high host specificity (Davison, 2002).…”

Section: Discussionmentioning

confidence: 99%

Detection of cyprinid herpesvirus 1 (CyHV-1) in barbel (Barbus barbus): First molecular evidence for the presence of CyHV-1 in fish other than carp (Cyprinus carpio)

Borzák

Sellyei

Baska

et al. 2020

AVet

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Two adult barbels (Barbus barbus) with visible skin tumours were subjected to histopathological and molecular examinations. The fish were caught in the River Danube near Budapest. Papillomas were found around their oral cavity, at the operculum and at the pectoral fins, while epidermal hyperplasias were seen on the body surface. Cyprinid herpesvirus 1 (CyHV-1) was detected in the kidney of the specimens by polymerase chain reaction (PCR), and barbel circovirus 1 (BaCV1) was found in all internal organs and in the tissues of the tumours. The whole genome of BaCV1 and three conserved genes from the genome of CyHV-1 were sequenced. Previously, BaCV1 had been reported only once from a mass mortality event among barbel fry. The whole genome sequence of our circovirus shared 99.9% nucleotide identity with that of the formerly reported BaCV1. CyHV-1 is known to infect common carp and coloured carp (Cyprinus carpio), and has been assumed to infect other cyprinid fish species as well. We found the nucleotide sequences of the genes of CyHV-1 to be identical in 98.7% to those of the previous isolates from carp. To the best of our knowledge, this is the first molecular confirmation of the presence of CyHV-1 DNA in cyprinid fish species other than carp.

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“…Five to ten mg of sliced FFPE tissue was placed in 1 mL of xylene (J.T. Baker, Phillipsburg, NJ, USA), and a pre-extraction step to remove paraffin from the sample was applied, as previously described [ 18 ]. Samples were deparaffinized in xylene for 5 min; following centrifugation, samples were washed twice in 100% ethanol.…”

Section: Methodsmentioning

confidence: 99%

Pathological and Tissue-Based Molecular Investigation of Granulomas in Cichlids Reared as Ornamental Fish

Mandrioli

Codotto

D’Annunzio

et al. 2022

Animals

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Cichlids include hundreds of species with a high economic value for aquaculture. These fish are subjected to intensive trade and farming that expose them to the risk of infectious diseases. This work focuses on ornamental cichlids held in an aquarium commercial facility presenting emaciation, in order to evaluate the presence of lesions in fish skin and organs. The fish were sampled during routine management activities and subjected to pathological and molecular investigations. The presence of lymphocystis disease virus, typically associated with cutaneous nodular disease, was ruled out. Histologically, they presented granulomas in the spleen, sometimes extending to the other visceral organs. Bacterial heat-shock protein 65 PCR products were detected in tissues associated, in the majority of cases, with granulomas; molecular investigation identified Mycobacterium spp. in two cases and Cutibacterium acnes in seven cases. Immunoreactivity to anti-Mycobacterium and anti-C. acnes antibodies was detected within granulomas. The presence of C. acnes within granuloma is elucidated for the first time in fish; however, similarly to what is found in humans, this bacterium could be harmless in normal conditions, whereas other contributing factors would be required to trigger a granulomatogenous response. Further confirmation by bacterial culture, as well as using large-scale studies in more controlled situations, is needed.

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Detection of Cyprinid Herpesvirus 1 DNA in cutaneous squamous cell carcinoma of koi carp (Cyprinus carpio)

Cited by 16 publications

References 31 publications

Immunohistochemical Study of Four Fish Tumors

Immunohistochemical Study of Four Fish Tumors

Detection of cyprinid herpesvirus 1 (CyHV-1) in barbel (Barbus barbus): First molecular evidence for the presence of CyHV-1 in fish other than carp (Cyprinus carpio)

Pathological and Tissue-Based Molecular Investigation of Granulomas in Cichlids Reared as Ornamental Fish

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