Detection of cytochrome P450 1A1 in North Sea dab liver and kidney

Abstract

Liver and kidney tissue of dab Limanda limanda were analyzed during the Bremerhaven Workshop by irnrnunochemical and catalytic assays for cytochrome P450 1Al induction. Antibodies to cod P450 1Al cross-reacted with a protein of M, = 58 000 daltons in the dab liver samples, suggested to be the dab P450 1Al protein. By employing a recently developed indirect ELISA wlth these antibodies, we observed elevated levels of P450 1Al protein in the innermost stations of the German Bight transect in both liver and kidney (head and trunk kidney) samples. Ethoxyresorufin 0-deethylase (EROD) activities in trunk kidney were also elevated in the innermost stations. Along the drilling station gradient no induction could be detected. Immuno-detection of the cytochrome P450 1Al induction response should provide a convenient indicator for biological effects of several classes of organic contaminants.

Optimization of a Cytochrome-P450-Monooxygenase-1A-Mediated EROD Assay in the Cape Hake Species Merluccius capensis and Merluccius paradoxus (Pisces)

“…Investigations by Mihailovic et al [ 34 ] on the hake, Merluccius merluccius, reported that the molecular weight of CYP1A was 55 kDa. Most studies present the CYP1A protein as a monomer [ 25 , 44 ]; therefore it was assumed that the lower molecular proteins observed in Figure 1 were degradation products formed during the isolation procedure.…”