Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry

Sun, Hongliu; Cai, Qianqian; Niu, Yu; Tai, Kang; Wei, Yongxin; Jin, Gang; Dong, Xiaohui; Wang, Chunhua; Zhu, Wei

doi:10.1371/journal.pone.0136253

Cited by 12 publications

(1 citation statement)

References 32 publications

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“…Ellipsometry, known as the best technique for surface studying, is also reported to be directly used to determine the biolayer, and various strategies, such as imaging ellipsometry and spectroscopic ellipsometry, are employed for biosensing applications [ 17 , 18 , 19 ]. It has become a practical technique for protein detection with functions of in situ, label-free, non-destructive, sensitive characterization for protein interaction process and quantification, and high-throughput measurements can be realized using imaging ellipsometry [ 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Fast and Sensitive Ellipsometry-Based Biosensing

Wang²,

Wang

et al. 2017

Sensors

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In this work, a biosensing method based on in situ, fast, and sensitive measurements of ellipsometric parameters (Ψ, ∆) is proposed. Bare silicon wafer substrate is functionalized and used to bind biomolecules in the solution. Coupled with a 45° dual-drive symmetric photoelastic modulator-based ellipsometry, the parameters Ψ and ∆ of biolayer arising due to biomolecular interactions are determined directly, and the refractive index (RI) of the solution and the effective thickness and surface mass density of the biolayer for various interaction time can be further monitored simultaneously. To illustrate the performance of the biosensing method, immunosensing for immunoglobulin G (IgG) was taken as a case study. The experiment results show that the biosensor response of the limit of detection for IgG is 15 ng/mL, and the data collection time is in milliseconds. Moreover, the method demonstrates a good specificity. Such technique is a promising candidate in developing a novel sensor which can realize fast and sensitive, label-free, easy operation, and cost-effective biosensing.

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Section: Introductionmentioning

confidence: 99%

Fast and Sensitive Ellipsometry-Based Biosensing

Wang²,

Wang

et al. 2017

Sensors

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Electrochemical Immunoassay for Cytomegalovirus Antigen Detection with Multiple Signal Amplification Using HRP and Pt‐Pd Nanoparticles Functionalized Single‐walled Carbon Nanohorns

et al. 2016

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Cytomegalovirus is typically associated with immunocompromised hosts, pregnant women and transplant patients, who require a timely diagnosis. In this work, a sensitive and highly specific electrochemical amplification immunosensor was established for detecting Cytomegalovirus pp65 antigen based on Pt‐PdNPs@SWCNHs with horseradish peroxidase (HRP) as a signal enhancer and thionine as a signal probe. First, Pt nanoparticle (PtNP) and Pd nanoparticle (PdNP) functionalized single‐walled carbon nanohorn (SWCNH) nanocomposites, i.e. Pt‐PdNPs@SWCNHs, was used as a carrier for immobilization of antibody through the Pt‐N bond and the Pd‐N bond. Next, HRP was used to block the rest of the binding‐sites. Signal amplification was obtained by the cooperative catalytic activities of Pt‐PdNPs and HRP to H2O2. SWCNHs loaded with a large amount of Pt‐PdNPs further amplified the signal due to the excellent surface area. The fabricated immunosensor was used to detect different concentrations of Cytomegalovirus pp65 antigen under optimized conditions. The tests showed a linear range from 0.1 to 80 ng mL−1 with a low detection limit of 30 pg mL−1, and exhibited excellent selectivity, stability and reproducibility. Therefore, this project presented a potential approach for the early diagnosis of Cytomegalovirus infection in clinical trials.

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Antibody-based magneto-elastic biosensors: potential devices for detection of pathogens and associated toxins

Menti

Henriques

Missell

et al. 2016

Appl Microbiol Biotechnol

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This work describes the design and development process of an immunosensor. The creation of such devices goes through various steps, which complement each other, and choosing an efficient immobilization method that binds to a specific target is essential to achieve satisfactory diagnostic results. In this perspective, the emphasis here is on developing biosensors based on binding antigens/antibodies on particular surfaces of magneto-elastic sensors. Different aspects leading to the improvement of these sensors, such as the antibody structure, the chemical functionalization of the surface, and cross-linking antibody reticulation were summarized and discussed. This paper deals with the progress of magneto-elastic immunosensors to detect bacterial pathogens and associated toxins. Biologically modified surface characterization methods are further considered. Thus, research opportunities and trends of future development in these areas are finally discussed.

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Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry

Cited by 12 publications

References 32 publications

Fast and Sensitive Ellipsometry-Based Biosensing

Fast and Sensitive Ellipsometry-Based Biosensing

Electrochemical Immunoassay for Cytomegalovirus Antigen Detection with Multiple Signal Amplification Using HRP and Pt‐Pd Nanoparticles Functionalized Single‐walled Carbon Nanohorns

Antibody-based magneto-elastic biosensors: potential devices for detection of pathogens and associated toxins

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