Detection of cytomegalovirus (CMV) by real-time PCR in fecal samples for the non-invasive diagnosis of CMV intestinal disease

Ganzenmueller, Tina; Kluba, Jeanette; Becker, Jan U.; Bachmann, Oliver; Heim, Albert

doi:10.1016/j.jcv.2014.10.009

Cited by 35 publications

(28 citation statements)

References 32 publications

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“…However, only scarce data has been published suggesting that the detection of DNA CMV in fecal samples might add some value for the differential diagnosis of diarrhea in immunocompromised patients and patients with inflammatory bowel disease (IBD). According to a study by Ganzenmueller et al 17 , conducted on immunocompromised patients (68% stem cell or solid organ transplantation), the PCR analysis from fecal samples were positive in 8 out of 12 patients with CMV enterocolitis. This data was confirmed by Michel et al 18 in a study on immunocompromised patients where all 4 patients with CMV enterocolitis were tested positive in the PCR analysis of fecal samples, with only one false positive result.…”

Section: Discussionmentioning

confidence: 99%

Detection of cytomegalovirus DNA in fecal samples in the diagnosis of enterocolitis after allogeneic stem cell transplantation

Zavřelová¹,

Radocha²,

Plíšková³

et al. 2018

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Section: Discussionmentioning

confidence: 99%

Detection of cytomegalovirus DNA in fecal samples in the diagnosis of enterocolitis after allogeneic stem cell transplantation

Zavřelová¹,

Radocha²,

Plíšková³

et al. 2018

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…In the recent studies, the detection of CMV by real-time PCR in stool samples was a reliable assay for the noninvasive diagnosis of CMV colitis (29,30). Since the general status of our patient was relatively stable when he showed the persistent clinical symptoms of colitis, he was able to undergo a colonoscopy and the endoscopic findings showed the possibility of CMV colitis, resulting in a definitive diagnosis.…”

Section: Discussionmentioning

confidence: 77%

“…Since the general status of our patient was relatively stable when he showed the persistent clinical symptoms of colitis, he was able to undergo a colonoscopy and the endoscopic findings showed the possibility of CMV colitis, resulting in a definitive diagnosis. If colonoscopy is not feasible in unstable critically ill patients, the detection of CMV in stool samples by a PCR might be helpful as a non-invasive diagnostic method (30).…”

Section: Discussionmentioning

confidence: 99%

Cytomegalovirus Colitis in a Critically Ill Patient Following Severe <i>Legionella</i> Pneumonia with Multiple Organ Failure

et al. 2016

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A 68-year-old man visited an emergency department complaining of dyspnea. He was diagnosed to have Legionella pneumonia with multiple organ failure. Although his multiple organ failure improved, he suffered from persistent abdominal pain and diarrhea with continuous minor bleeding. Colonoscopy revealed a longitudinal ulcer of the rectum, below the peritoneal reflection. He was diagnosed with cytomegalovirus (CMV) colitis. Antiviral therapy with ganciclovir was initiated. He finally underwent a colostomy after a bowel stricture caused an intestinal outlet obstruction, which made oral intake impossible. Based on the present case, we believe that CMV colitis must be considered as one of the differential diagnoses when critically ill patients develop continuous diarrhea and abdominal pain.

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“…7,8,10,11,20 On the other hand, CMV gastrointestinal symptoms could be an important manifestation of postnatal infection in patients admitted to neonatal intensive care units 25 or a manifestation of CMV intestinal disease in immunocompromised patients or patients affected by inflammatory bowel disease. 22,[26][27][28][29][30][31][32][33] A rare presentation with intestinal polyps in immunocompetent healthy patients has also been described. 34 …”

Section: Introductionmentioning

confidence: 95%

Neonatal gastrointestinal involvement and congenital cytomegalovirus

et al. 2016

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Cytomegalovirus (CMV) is the most common cause of congenital viral infection, affecting 0.2 to 2.3% of all live births in developed countries. Very low birth weight and extremely low birth weight newborns are at higher risk of symptomatic CMV infection, most commonly secondary and acquired through breast milk. Gastrointestinal involvement is rare in acquired CMV infections, but it could be an important manifestation of postnatal infection in preterm infants admitted to neonatal intensive care units. Early onset of CMV gastrointestinal signs/symptoms is very rare. In a review of the literature it is described in 5 newborns in the first 24 hours of life, and 6 considering the onset in the first week of life. This review describes also a case report of congenital CMV in an immunocompetent newborn with onset of gastrointestinal signs immediately after birth: a possible association between viral infection and enteric manifestations was considered in the differential diagnosis. A review of the literature of the different case reports found has done, with description and comparison of the different patients and clinical presentations. IntroductionCytomegalovirus (CMV) is the most common cause of congenital viral infection, affecting 0.2 to 2.3% of all live births in developed countries; 1-12 it is also estimated as the leading infectious cause of nongenetic hearing loss, mental retardation 1,2,13-15 and non-genetic congenital malformations. 16 Very low birth weight and extremely low birth weight newborns are at higher risk of symptomatic CMV infection, most commonly secondary, acquired through breast milk. 17 Maternal primary CMV infection or reinfection during pregnancy are the cause of neonatal congenital infection, with a mean risk of transmission of 40% (24-75%), 3,18 lower in the first three months (36%) than in the last three months of gestation (78%). 3,19 Considering all the infected newborns, only 5 to 10% are symptomatic at birth, in particular with neurodevelopmental delay signs and sensorial hearing loss. Among asymptomatic patients, 10% to 15% will show developmental disorders, mainly sensory hearing loss. 1,2,8,10,11,13,14,[20][21][22] Most of congenital infections (85-90%) remain asymptomatic: 3,16,23 among them, 8 to 15% will show developmental disorders or sensorial hearing loss. [1][2][3]13,14 Possible signs and symptoms of congenital CMV infection with onset at birth are the following: 3,16,24 unilateral or bilateral sensorial hearing loss, visual loss, microcephaly, hepatomegaly and/or hepatitis, splenomegaly, thrombocytopenia, jaundice, petechiae, motor defects, mental disability, chorioretinitis, strabismus, optic atrophy, dental defects. Rarely it could present as a severe multi organ dysfunction with polyserositis.Gastrointestinal involvement is considered very rare in congenital and acquired CMV infection. 7,8,10,11,20 On the other hand, CMV gastrointestinal symptoms could be an important manifestation of postnatal infection in patients admitted to neonatal intensive care units 25 or a ...

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Detection of cytomegalovirus (CMV) by real-time PCR in fecal samples for the non-invasive diagnosis of CMV intestinal disease

Cited by 35 publications

References 32 publications

Detection of cytomegalovirus DNA in fecal samples in the diagnosis of enterocolitis after allogeneic stem cell transplantation

Detection of cytomegalovirus DNA in fecal samples in the diagnosis of enterocolitis after allogeneic stem cell transplantation

Cytomegalovirus Colitis in a Critically Ill Patient Following Severe <i>Legionella</i> Pneumonia with Multiple Organ Failure

Neonatal gastrointestinal involvement and congenital cytomegalovirus

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