Detection of Cytomegalovirus in Bronchoalveolar Lavage Fluid from HIV-Positive Individuals with Community Acquired Pneumonia

Mane, Arati; Gujar, Pankaj; Gaikwad, Shraddha; Dhamgaye, Tilak; Risbud, Arun

doi:10.7860/jcdr/2017/28148.10302

Cited by 2 publications

(3 citation statements)

References 20 publications

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“…It is potentially helpful for obtaining tissue samples for diagnosing various pulmonary infections resulting from opportunistic pathogens such as Pneumocystis jirovecii , Cytomegalovirus (CMV), and Aspergillus species, as these have a significant alveolar component. Besides the cytomorphologic evaluation, the BAL samples can also be used for performing a wide array of confirmatory molecular diagnostic tests thereby aiding in prompt patient management 6–14 …”

Section: Discussionmentioning

confidence: 99%

“…Besides the cytomorphologic evaluation, the BAL samples can also be used for performing a wide array of confirmatory molecular diagnostic tests thereby aiding in prompt patient management. [6][7][8][9][10][11][12][13][14] Cytological and histological identification of the characteristic cytopathic intranuclear inclusions in the affected cells is diagnostic. Thus, the presence of the viral cytopathic changes in infected pneumocytes in an appropriate inflammatory background is virtually diagnostic of CMV pneumonitis; however, is less sensitive.…”

Section: Case Presentationmentioning

confidence: 99%

See 1 more Smart Citation

The need for “eagle‐eyed screening”: Owl's eyes in bronchoalveolar lavage cytology

Duggal,

Gupta,

Ambalavanan

et al. 2024

Diagnostic Cytopathology

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Section: Discussionmentioning

confidence: 99%

Section: Case Presentationmentioning

confidence: 99%

The need for “eagle‐eyed screening”: Owl's eyes in bronchoalveolar lavage cytology

Duggal,

Gupta,

Ambalavanan

et al. 2024

Diagnostic Cytopathology

View full text Add to dashboard Cite

“…A previous study has suggested that BAL could be a useful tool in the identification of pathogens in ventilator-associated pneumonia, or in acute hypoxemic respiratory failure which requires noninvasive ventilation [31]. Another literature also has elucidated the utility of BAL acting as a less invasive option for the assessment of lung pathology, which could also aids in the diagnosis of Cytomegalovirus pneumonitis using molecular methods [32]. Therefore, BAL is an accurate and safe method to validate pathogens in pneumonia.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of the PI3K/AKT Signaling Pathway or Overexpression of Beclin1 Blocks Reinfection of Streptococcus pneumoniae After Infection of Influenza A Virus in Severe Community-Acquired Pneumonia

et al. 2019

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Streptococcus pneumoniae (S. pneumoniae) and viruses are considered as primary risks of community-acquired pneumonia (CAP), and the effects of co-infection bacterial and virus in the prognosis of patients with severe CAP (SCAP) are poorly described. Therefore, this study is conducted to investigate the regulation of Beclin1-PI3K/AKT axis in reinfection of S. pneumoniae after influenza A virus in mice model of bronchoalveolar lavage fluid (BALF). Samples of sputum and BALF were collected from patients with SCAP for etiological detection. The expression of each gene was determined by RT-qPCR and western blot analysis. Influenza A/PR/8/34 and S. pneumoniae were used to establish the mice model of reinfection pneumonia. The virus quantity, expression levels of inflammatory factors, bacterial load, and myeloperoxidase (MPO) activity were tested. HE staining was applied to observe histopathology of lung tissue. The expression of Beclin1 was downregulated and the PI3K/AKT pathway was activated in viral pneumonia. In vivo experiment, the reinfection of S. pneumoniae following influenza A virus infection increased the number of S. pneumoniae population, the activity of MPO, and the expression of TNF-α, IL-6, and IFN-γ in BALF of mice. In contrast, inhibition of the PI3K/AKT pathway or overexpression of Beclin1 reduced the number of S. pneumoniae population, the activity of MPO, and the expression of TNF-α, IL-6, and IFN-γ in BALF of mice reinfected with S. pneumoniae after influenza A virus infection. Collectively, our study demonstrates that inhibition of the PI3K/AKT signaling pathway or overexpressed Beclin1 alleviates reinfection of S. pneumoniae after influenza A virus infection in SCAP.

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Detection of Cytomegalovirus in Bronchoalveolar Lavage Fluid from HIV-Positive Individuals with Community Acquired Pneumonia

Cited by 2 publications

References 20 publications

The need for “eagle‐eyed screening”: Owl's eyes in bronchoalveolar lavage cytology

The need for “eagle‐eyed screening”: Owl's eyes in bronchoalveolar lavage cytology

Inhibition of the PI3K/AKT Signaling Pathway or Overexpression of Beclin1 Blocks Reinfection of Streptococcus pneumoniae After Infection of Influenza A Virus in Severe Community-Acquired Pneumonia

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