Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervical Carcinoma (C33-A)

Abstract

Lectins comprise a heterogeneous class of proteins that recognize the carbohydrate moieties of glycoconjugates with high specificity. Numerous studies have shown that lectins are capable of recognizing specific carbohydrate moieties displayed by malignant cells or tissues. The present work was performed to investigate the effects of tepary bean (Phaseolus acutifolius) lectins on proliferation, colony formation, and alteration of DNA synthesis of human malignant cells. Tepary bean lectin showed dose dependent effects on the inhibition of viability as well as on colony formation in two human malignant cells lines (C33-A, Sw480); By contrast, tepary bean lectin only showed significant effects on DNA synthesis on Sw480 cells. Our results provide evidence of the anti- proliferative and cytotoxic effects of the tepary bean lectins on C33-A and Sw480 cells lines.

Tepary Bean (Phaseolus acutifolius) Lectins Induce Apoptosis and Cell Arrest in G0/G1 by P53(Ser46) Phosphorylation in Colon Cancer Cells

“…A Tepary bean (Phaseolus acutifolius) lectin fraction (TBLF) has shown differential cytotoxic effects on breast, cervix and colon cancer cell lines as well as on non-malignant cells from the intestinal tract [15,18]. Similar effects were observed with other Tepary bean lectin fractions on different colon cancer cell lines [19,20]. In vivo studies have reported that TBLF (50 mg/kg by intragastric administration for six weeks every third day) exhibits low toxicity, good tolerability and activates the immune system [21,22].…”

Isolation of a Glucosamine Binding Leguminous Lectin with Mitogenic Activity towards Splenocytes and Anti-Proliferative Activity towards Tumor Cells

“…On the other hand, several Phaseolus lectins had anti-tumor activity on colon cancer cell lines like Caco-2 cells [16], [17], while some other Phaseolus lectins were inhibitory cervical cancer HeLa cells [18]. However, lectin from tepary bean could inhibit viability of both colon cancer Sw480 cells and cervical cancer C33-A cells [19]. As different P. vulgaris lectins possess different biological activities, investigation of new P. vulgaris lectins would provide chances for identification of lectins with good potential for therapeutic uses.…”

Potential role of bioactive peptides in prevention and treatment of chronic diseases: a narrative review

“…Another group of plant‐derived bioactive proteins is represented by lectins, which are able to recognize specific carbohydrate moieties displayed by malignant cells or tissues. For example, tepary bean ( Phaseolus acutifolius ) lectins have demonstrated antiproliferative and cytotoxic effects on the cervical carcinoma cell line, C33‐A, and human colon carcinoma cell line, Sw480, by the inhibition of colony formation (Valadez‐Vega et al , ). Lectins derived from mistletoe have been studied in a mouse model and demonstrated an immunomodulatory effect that delayed the development of colon cancer (Ma et al , ) and improved the survival of mice with leukaemia cells (Seifert et al , ).…”