Detection of Cytotoxicity and Carcinogenicity of Soft Drink "Cola" on Allium Cepa Root Cells

Abstract

The present investigation had been conducted to study the cytogenetic effects of soft drink "cola " at concentration (100%) with three exposure times (4, 6, and 8 hours) on root meristems of Allium cepa. The parameters tested were: mitotic index and chromosomal aberrations. Mitotic index was reduced in all exposure times 4,6 and 8 h with cola. On the other hand, total chromosomal abnormalities were highly increased in all exposure times comparatively with control. The physiological chromosomal aberrations observed were: (c-mitosis, stickiness, disturbance and laggard chromosome; and clastogenic aberrations were: chromosomal break and bridges. These findings indicate the cytotoxicity and carcinogenicity of the soft drink "cola". Our results supports the calls for warned for excessive intake of soft drinks especially "cola".

Assessment of genotoxic and mutagenic effects of food products by bioassay methods

“…Mitotoxicity, genotoxicity and mutagenicity were recorded in analysis of the other frequently used soft drink, Coca-Cola, in the experimental conditions using the onion apical meristem [28][29][30]. The mitotic index decreased in a dose-dependent manner with the extension of treatment duration and the level of aberrant cells increased when roots were treated with soft drinks.…”

Developing the Methods of Food Preservatives Extraction From Complex Matrices for Bioassay Purpose

“…Currently, the Allium test is widely and successfully used to study the toxic, cytotoxic and genotoxic effects of food additives [6]. Several papers have also been published, in which onion roots were placed directly in food products such as drinks, juices, and milk [7,8,9]. However, these experiments are not so unambiguous, because high concentration of carbohydrates in drinks and juices, and the presence of emulsion in milk are undesirable factors in the Allium test.…”