Detection of daptomycin-nonsusceptible strains using the Neo-Sensitab™ prediffusion method

Katz, Bradley D.; Luperchio, Steven A.; Thorne, Grace M.

doi:10.1016/j.diagmicrobio.2008.01.021

Cited by 6 publications

(3 citation statements)

References 7 publications

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“…The prediffusion method has been described for the detection of colistin-resistance, daptomycin-nonsusceptible strains, and the hVISA/VISA phenotype. 2 , 23 , 32 Although this screening method is not expensive and might represent a simpler alternative for hVISA detection in clinical laboratories, it has not been extensively evaluated. Despite the two false positives, we had acceptable results with the prediffusion method (2/3 hVISA isolates detected).…”

Section: Discussionmentioning

confidence: 99%

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Clinical, Microbiological, and Genetic Characteristics of Heteroresistant Vancomycin-Intermediate Staphylococcus aureus Bacteremia in a Teaching Hospital

Gregorio

Perazzi

Ordoñez

et al. 2015

Microbial Drug Resistance

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The emergence of vancomycin intermediate Staphylococcus aureus (VISA) and heterogeneous VISA (hVISA) is of major concern worldwide. Our objective was to investigate the prevalence, phenotypic and molecular features of hVISA strains isolated from bacteremic patients and to determine the clinical significance of the hVISA phenotype in patients with bacteremia. A total of 104 S. aureus blood isolates were collected from a teaching hospital of Argentina between August 2009 and November 2010. No VISA isolate was recovered, and 3 out of 92 patients (3.3%) were infected with hVISA, 2 of them methicillin-resistant S. aureus (MRSA) (4.5% of MRSA). Macro Etest and prediffusion method detected 3/3 and 2/3 hVISA respectively. Considering the type of bacteremia, the three cases were distributed as follows: two patients had suffered multiple episodes of bacteremia (both hVISA strains recovered in the second episode), while only one patient had suffered a single episode of bacteremia with hVISA infection. MRSA bloodstream isolates exhibiting the hVISA phenotype were related to HA-MRSA Cordobes clone (ST5-SCCmec I-spa t149) and MRSA Argentinean pediatric clone (ST100-SCCmec IVNV-spa t002), but not to CA-MRSA-ST30-SCCmec IV-spa t019 clone that was one of the most frequent in our country. Although still relatively infrequent in our hospital, hVISA strains were significantly associated with multiple episodes of bacteremia (p=0.037) and genetically unrelated.

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Section: Discussionmentioning

confidence: 99%

“…The results were interpreted following the manufacturer's recommendation: vancomycin inhibition zone diameter ≤22 mm and/or teicoplanin inhibition zone diameter <20 mm were considered as a positive result. 23 , 38…”

Section: Methodsmentioning

confidence: 99%

Clinical, Microbiological, and Genetic Characteristics of Heteroresistant Vancomycin-Intermediate Staphylococcus aureus Bacteremia in a Teaching Hospital

Gregorio

Perazzi

Ordoñez

et al. 2015

Microbial Drug Resistance

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“…Even though this is not the first report of the 15 isolates with acquired resistance, belonging to the population with the smaller inhibition zones. Further improvements as done byKatz et al (2008), namely using a shorter second incubation period (6 hours instead of 18 hours), may offer a faster and more As many laboratories still rely on the cheaper disk diffusion test, the emergence of colistin resistance may be missed, as demonstrated in this study. It is clear that this type of resistance needs to be monitored closely, using the appropriate test methods.…”

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confidence: 88%

Disk prediffusion is a reliable method for testing colistin susceptibility in porcine E. coli strains

Boyen

Vangroenweghe²,

Butaye

et al. 2010

Veterinary Microbiology

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19During the last few years, acquired resistance to colistin in , but also in other 20 bacterial species, has been reported. It disk diffusion assay, suggesting acquired resistance in 15 strains (9.6 %). The E-test and disk 29 prediffusion assay generated results within acceptable levels compared to the reference agar 30 dilution assay. The categorical agreement with the results obtained the agar dilution 31 method were good to very good for all tests, except the disk diffusion assay. In conclusion, 32 current results suggest that, in addition to the E-test, the disk prediffusion test is a reliable, 33 alternative agar-based colistin susceptibility method for testing colistin susceptibility of 34 isolates in diagnostic bacteriology.

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Detection of daptomycin-nonsusceptible strains using the Neo-Sensitab™ prediffusion method

Cited by 6 publications

References 7 publications

Clinical, Microbiological, and Genetic Characteristics of Heteroresistant Vancomycin-Intermediate Staphylococcus aureus Bacteremia in a Teaching Hospital

Clinical, Microbiological, and Genetic Characteristics of Heteroresistant Vancomycin-Intermediate Staphylococcus aureus Bacteremia in a Teaching Hospital

Disk prediffusion is a reliable method for testing colistin susceptibility in porcine E. coli strains

Daptomycin In Vitro Susceptibility Methodology: a Review of Methods, Including Determination of Calcium in Testing Media

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