Detection of dengue virus serotype 3 by reverse transcription-polymerase chain reaction in Aedes aegypti (Diptera, Culicidae) captured in Manaus, Amazonas

Pinheiro, Valéria Cristina Soares; Tadei, Wanderli Pedro; Barros, Patricia M. S. S.; Vasconcelos, Pedro F. C.; Cruz, Ana Cecília Ribeiro

doi:10.1590/s0074-02762005000800003

Cited by 30 publications

(22 citation statements)

References 31 publications

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“…These results are in agreement with those of other fi eld studies, such as the one carried out by Chow et al, 1 which failed to detect dengue virus in 53 pools of larvae (of 1 to 10 individuals each) collected during household visits. Pinheiro et al, 11 in a study carried out in the city of Manaus, failed to detect dengue virus in 1.142 larvae collected during household visits, although these authors obtained an MIR of 16 males collected in the fi eld. These results suggest that transovarial transmission of dengue virus in Ae.…”

Section: Discussionmentioning

confidence: 97%

Vírus dengue em larvas de Aedes aegypti e sua dinâmica de infestação, Roraima, Brasil

Zeidler¹,

Acosta²,

Barrêto³

et al. 2008

Rev. Saúde Pública

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OBJECTIVE:To detect the presence of dengue virus in larval forms of Aedes aegypti and to associate vector presence with rainfall and incidence of disease. METHODS:Eighteen households were randomly selected for egg collection in a neighborhood of the city of Boa Vista, Roraima, in Northern Brazil. Two oviposition traps were installed per home, and removed after one week. This was repeated on a monthly basis between November 2006 and May 2007. Trap positivity rate and egg density were calculated. Following the eclosion of 1,422 eggs, 44 pools of at least 30 larvae each were formed, which were evaluated for presence of dengue virus using RT-PCR and hemi-nested PCR. Dengue incidence rates in the period were correlated with rainfall rates. The association between these two variables and the number of eggs collected was determined using Pearson correlation. RESULTS:None of the pools tested positive for presence of dengue virus, despite the high incidence of dengue in the neighborhood during the studied period. The density of Ae. aegypti increased with rainfall, but was not correlated with incidence of dengue. CONCLUSIONS:The results suggest that transovarial transmission of dengue virus in mosquitoes occurs at a very low frequency, and therefore virus persistence in urban settings may not depend on such transmission. The mosquito population increased during the rainy season due to increased formation of breeding sites; the lack of correlation with incidence of dengue may be due to underestimation of incidence data during epidemics.

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Section: Discussionmentioning

confidence: 97%

Vírus dengue em larvas de Aedes aegypti e sua dinâmica de infestação, Roraima, Brasil

Zeidler¹,

Acosta²,

Barrêto³

et al. 2008

Rev. Saúde Pública

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“…aegypti females were reported in the home environment in Southeast Asia [11][12][13][14][15][16][17][18][19][20] and the Americas. 7,[21][22][23][24][25][26][27] For example, we recently showed that DENV-infected Ae. aegypti females are commonly found in the homes of laboratory-confirmed dengue patients in Mérida, México, up to 4 weeks after onset of symptoms.…”

Section: Introductionmentioning

confidence: 99%

Mosquito Infestation and Dengue Virus Infection in Aedes aegypti Females in Schools in Mérida, México

García-Rejón¹,

Loroño-Pino²,

Farfán-Ale³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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We determined abundance of Aedes aegypti mosquitoes and presence of dengue virus (DENV) in females collected from schools in Mérida, México, during 2008 and 2009. Backpack aspiration from 24 schools produced 468 females of Ae. aegypti and 1,676 females of another human biter, Culex quinquefasciatus. Ae. aegypti females were collected most commonly from classrooms followed by offices and bathrooms. Of these females, 24.7% were freshly fed. Examination of 118 pools of Ae. aegypti females (total of 415 females) for presence of DENV RNA produced 19 positive pools (16.1%). DENV-infected pools were detected from 11 (45.8%) of 24 schools and came from different room types, including classrooms, offices, and bathrooms. The overall rate of DENV infection per 100 Ae. aegypti females was 4.8. We conclude that schools in Mérida present a risk environment for students, teachers, and other personnel to be exposed to mosquitoes and bites of DENV-infected Ae. aegypti females.

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“…aegypti females is feasible, and thus can serve as the basis for viable virological outcome measures in intervention studies. 9,22,[53][54][55][56][57][58] In conclusion, the longevity of Ae. aegypti, its penchant for feeding frequently on humans even in a single gonotrophic cycle, and its preference for feeding on humans in domiciles and in bedrooms where DENV-infected humans typically are cared for and visited by friends and relatives make it a public health imperative to control the mosquito in the home and other indoor environments.…”

mentioning

confidence: 99%

Towards a Casa Segura: A Consumer Product Study of the Effect of Insecticide-Treated Curtains on Aedes aegypti and Dengue Virus Infections in the Home

Loroño-Pino¹,

García-Rejón²,

Machaín-Williams³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. The home, or domicile, is the principal environment for transmission of dengue virus (DENV) between humans and mosquito vectors. Community-wide distribution of insecticide-treated curtains (ITCs), mimicking vector control program-driven interventions, has shown promise to reduce DENV infections. We conducted a Casa Segura consumer product intervention study in Mérida, Mexico to determine the potential to reduce intradomicillary DENV transmission through ITC use in individual homes. Dengue virus infections in mosquitoes and in humans were reduced in homes with ITCs in one of two study subareas. Overall, ITCs reduced intradomicillary DENV transmission; ITC homes were significantly less likely to experience multiple DENV infections in humans than NTC homes. Dengue virus-infected Aedes aegypti females were reduced within the ITC homes where curtain use was highest. Some homes yielded up to nine infected Ae. aegypti females. This study provides insights regarding best practices for Casa Segura interventions to protect homes from intradomicillary DENV transmission.

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Detection of dengue virus serotype 3 by reverse transcription-polymerase chain reaction in Aedes aegypti (Diptera, Culicidae) captured in Manaus, Amazonas

Cited by 30 publications

References 31 publications

Vírus dengue em larvas de Aedes aegypti e sua dinâmica de infestação, Roraima, Brasil

Vírus dengue em larvas de Aedes aegypti e sua dinâmica de infestação, Roraima, Brasil

Mosquito Infestation and Dengue Virus Infection in Aedes aegypti Females in Schools in Mérida, México

Towards a Casa Segura: A Consumer Product Study of the Effect of Insecticide-Treated Curtains on Aedes aegypti and Dengue Virus Infections in the Home

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