Detection of Deoxyribonuclease I in a Hormone-secretory Pathway of Pituitary Cells in Humans and Rats.

Shimada, Osamu; Suzuki, Shosuke; Tosaka-Shimada, Hisami; Ishikawa, Harunori

doi:10.1247/csf.23.49

Cited by 9 publications

(8 citation statements)

References 32 publications

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“…Lacks [14] Takeshita et al [ pituitary gland in human and rat. Shimada et al [46] immunolocalized DNASE1 in the hormone-secreting cells in the anterior and intermediate lobes.…”

Section: Discussionmentioning

confidence: 99%

Expression pattern of the deoxyribonuclease 1 gene: lessons from the Dnase1 knockout mouse

Napirei

Ricken

Eulitz

et al. 2004

Biochemical Journal

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The tissue distribution of deoxyribonuclease 1 (DNASE1, DNase I), a Ca2+ and Mg2+/Mn2+-dependent secretory endonuclease, has previously been investigated. However, most of these studies did not account for the existence of different members of the DNASE1 gene family, did not differentiate between endogenous DNASE1 protein synthesis and its extracellular occurrence or were not performed with methods allowing both a sensitive and a specific detection. Now we re-examined the DNASE1 gene expression pattern by taking advantage of the Dnase1 knockout mouse model. Direct comparison of samples derived from wild-type (Dnase1+/+) and knockout (Dnase1-/-) mice allowed an unambiguous detection of Dnase1 gene expression at the mRNA and protein level. For the detection of Dnase1 activity, we developed a highly sensitive nuclease zymogram method. We observed high Dnase1 gene expression in the parotid and submandibular gland as well as in the kidney and duodenum, intermediate expression in the ileum, mesenterial lymph nodes, liver, ventral prostate, epididymis, ovary and stomach, and low expression in the sublingual, preputial, coagulation and pituitary gland. We report for the first time the lachrymal and thyroid glands, the urinary bladder and the eye to be Dnase1-expressing organs as well. Since Dnase1 knockout mice with the 129xC57Bl/6 mixed genetic background have indicated the protection against an anti-DNA autoimmune response as a new physiological function of Dnase1, knowledge of the physiological sites of its synthesis might prove helpful to find new therapeutic strategies.

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“…Lacks [14] Takeshita et al [ pituitary gland in human and rat. Shimada et al [46] immunolocalized DNASE1 in the hormone-secreting cells in the anterior and intermediate lobes.…”

Section: Discussionmentioning

confidence: 99%

Expression pattern of the deoxyribonuclease 1 gene: lessons from the Dnase1 knockout mouse

Napirei

Ricken

Eulitz

et al. 2004

Biochemical Journal

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“…FITC-and Texas-Red-labeled phalloidins were purchased from Sigma (USA). An anti-type V myosin antibody was a gift from Dr. R. Ishikawa (Gunma University School of Medicine) (Ishikawa et al, 1998). Nocodazole, vinblastine, colcemid, cyotochalasins B and D (Wako Pure Chem., Japan) and latrunculin-B (Alexis Corporation, USA) were purchased, rapidly dissolved with 10% DMSO in PBS and these stock solutions were stored at -80°C in darkness.…”

Section: Reagents and Antibodiesmentioning

confidence: 99%

“…Cells cultured on glass slides were fixed for 5 min with 5% acrolein in 1/15 M phosphate buffer (PB), pH 7.4, at 37°C, washed three times with PB, then fixed with PB containing 1% paraformaldehyde, 0.125% glutaraldehyde and 0.1% picric acid for 1 hr at 37°C followed by 3 hr at 4°C (Shimada et al, 1998;2003). The cells were postfixed with 1% osmium tetroxide and 7% sucrose in PB for 1 hr at 4°C, dehydrated with a graded ethanol series at 0°C, embedded in Lowicryl K4M and cured under ultraviolet light for 3 days at -35°C.…”

Section: Immunoelectron Microscopymentioning

confidence: 99%

Clusters of VIP36-Positive Vesicles between Endoplasmic Reticulum and Golgi Apparatus in GH3 Cells

Shimada

Hara-Kuge²,

Yamashita³

et al. 2003

Cell Struct. Funct.

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ABSTRACT. The vesicular integral membrane protein VIP36 belongs to the family of animal lectins and may act as a cargo receptor trafficking certain glycoproteins in the secretory pathway. Immunoelectron microscopy of GH3 cells provided evidence that endogenous VIP36 is localized mainly in 70-100-nm-diameter uncoated transport vesicles between the exit site on the ER and the neighboring cis-Golgi cisterna. The thyrotrophinreleasing hormone (TRH) stimulation and treatment with actin filament-perturbing agents, cytochalasin D or B or latrunculin-B, caused marked aggregation of the VIP36-positive vesicles and the appearance of a VIP36-positive clustering structure located near the cis-Golgi cisterna. The size of this structure, which comprised conspicuous clusters of VIP36, depended on the TRH concentration. Confocal laser scanning microscopy confirmed the electron microscopically demonstrated distribution and redistribution of VIP36 in these cells. Furthermore, VIP36 colocalized with filamentous actin in the paranuclear Golgi area and its vicinity. This is the first study to show the ultrastructural distribution of VIP36 in the early secretory pathway in GH3 cells. It suggests that actin filaments are involved in glycoprotein transport between the ER and cis-Golgi cisterna by using the lectin VIP36.

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“…Each PVDF membrane was processed for immunoblot analysis using a polyclonal antibody produced against a synthesised peptide with 15 amino acids of the N-terminus (nos. 6-20) of rat DNase I (Shimada et al, 1998). The antibody was produced by immunising rabbits.…”

Section: Assay Of Dnasementioning

confidence: 99%

Enzymes responsible for the bactericidal effect in extracts of vitelline and fertilisation envelopes of rainbow trout eggs

Kudo

2000

Zygote

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Extracts from both the vitelline envelope (VE) and fertilisation envelopes (FE) of rainbow trout eggs have the ability to exert a bactericidal effect on Gram-positive and -negative bacteria. The effect may be due to the presence of phospholipase D (PLD), lysozyme, proteinase and DNases, as the extracts contain these enzyme activities. The intensity of chorionic PLD and lysozyme activities in the VE extract was maintained in the FE without any alteration in activity even after transformation in the course of the cortical reaction, as components of a fundamental architecture of the envelope. Both extracts also contain different types of proteinase activities. Treatment with VE or FE extract seriously damaged the outer membrane of Gram-negative bacteria and the plasma membrane of Gram-positive and -negative bacteria at the ultrastructural level. Chorionic DNases probably degrade DNA of bacterial cells killed by virtue of the action of PLD and/or lysozyme and contribute to the transmigration of nucleosides and/or nucleotides produced by degrading bacterial DNA after degradation of bacterial components by the actions of the chorionic PLD, lysozyme and proteinase. These results suggest that the bactericidal process manifested by the VE or FE extract may start with the action of PLD and/or lysozyme against bacteria and be completed by subsequent degradation of constitutive proteins and DNA by the action of proteinases and DNases, respectively. Thus the VE and FE are able to protect the egg itself and the embryo, respectively, from bacterial infection in the internal or external environments.

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Detection of Deoxyribonuclease I in a Hormone-secretory Pathway of Pituitary Cells in Humans and Rats.

Cited by 9 publications

References 32 publications

Expression pattern of the deoxyribonuclease 1 gene: lessons from the Dnase1 knockout mouse

Expression pattern of the deoxyribonuclease 1 gene: lessons from the Dnase1 knockout mouse

Clusters of VIP36-Positive Vesicles between Endoplasmic Reticulum and Golgi Apparatus in GH3 Cells

Enzymes responsible for the bactericidal effect in extracts of vitelline and fertilisation envelopes of rainbow trout eggs

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